Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2,5-dioxopentanoate + NAD(P)+ + H2O
2-oxoglutarate + NAD(P)H
2,5-dioxopentanoate + NAD+ + H2O
2-oxoglutarate + NADH + H+
2,5-dioxopentanoate + NADP+ + H2O
2-oxoglutarate + NADPH + 2 H+
2-oxoglutaric semialdehyde + NAD(P)+ + H2O
2-oxoglutarate + NAD(P)H + H+
-
-
-
?
2-oxoglutaric semialdehyde + NAD+ + H2O
2-oxoglutarate + NADH + H+
-
-
-
?
3-hydroxypropionaldehyde + NAD+
3-hydroxypropionate + NADH + H+
3-hydroxypropionaldehyde + NADP+
3-hydroxypropionate + NADPH + H+
very low activity
-
-
?
acetaldehyde + NAD(P)+ + H2O
acetate + NAD(P)H + H+
-
-
-
?
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O
alpha-ketoglutarate + NAD(P)H + H+
alpha-ketoglutaric semialdehyde + NADP+ + H2O
alpha-ketoglutarate + NADPH + H+
-
-
-
?
butyraldehyde + NAD(P)+ + H2O
n-butanoate + NAD(P)H + H+
-
-
-
-
?
butyraldehyde + NAD+
butanoate + NADH + H+
-
-
-
?
DL-glyceraldehyde + NADP+ + H2O
glycerate + NADPH + H+
glutaraldehyde + NAD(P)+ + H2O
glutarate + NAD(P)H + H+
-
-
-
?
glutaric semialdehyde + NADP+ + H2O
glutarate + NADPH
-
-
-
?
glycolaldehyde + NADP+ + H2O
glycolate + NADPH + H+
heptaldehyde + NAD(P)+ + H2O
n-heptanoate + NAD(P)H + H+
-
-
-
-
?
hexaldehyde + NAD+
hexanoate + NADH + H+
-
-
-
?
hexylaldehyde + NAD(P)+ + H2O
n-hexanoate + NAD(P)H + H+
-
-
-
-
?
octylaldehyde + NAD(P)+ + H2O
n-octanoate + NAD(P)H + H+
-
-
-
-
?
propanal + NAD(P)+ + H2O
propionate + NAD(P)H + H+
-
-
-
?
propionaldehyde + NAD(P)+ + H2O
propionate + NAD(P)H + H+
-
-
-
-
?
propionaldehyde + NAD+
propionate + NADH + H+
-
-
-
?
succinate semialdehyde + NAD(P)+ + H2O
? + NAD(P)H + H+
-
-
-
?
succinate semialdehyde + NAD+ + H2O
succinate + NADH + H+
valeraldehyde + NAD(P)+ + H2O
n-pentanoate + NAD(P)H + H+
-
-
-
-
?
valeraldehyde + NAD+
valerate + NADH + H+
-
-
-
?
additional information
?
-
2,5-dioxopentanoate + NAD(P)+ + H2O

2-oxoglutarate + NAD(P)H
-
-
-
?
2,5-dioxopentanoate + NAD(P)+ + H2O
2-oxoglutarate + NAD(P)H
-
-
-
?
2,5-dioxopentanoate + NAD(P)+ + H2O
2-oxoglutarate + NAD(P)H
-
-
-
?
2,5-dioxopentanoate + NAD(P)+ + H2O
2-oxoglutarate + NAD(P)H
-
-
-
?
2,5-dioxopentanoate + NAD(P)+ + H2O
2-oxoglutarate + NAD(P)H
-
-
-
?
2,5-dioxopentanoate + NAD(P)+ + H2O
2-oxoglutarate + NAD(P)H
-
-
-
?
2,5-dioxopentanoate + NAD(P)+ + H2O
2-oxoglutarate + NAD(P)H
-
-
-
?
2,5-dioxopentanoate + NAD(P)+ + H2O
2-oxoglutarate + NAD(P)H
-
-
-
?
2,5-dioxopentanoate + NAD+ + H2O

2-oxoglutarate + NADH + H+
-
-
-
r
2,5-dioxopentanoate + NAD+ + H2O
2-oxoglutarate + NADH + H+
-
-
-
r
2,5-dioxopentanoate + NAD+ + H2O
2-oxoglutarate + NADH + H+
-
-
-
r
2,5-dioxopentanoate + NAD+ + H2O
2-oxoglutarate + NADH + H+
-
-
-
-
r
2,5-dioxopentanoate + NAD+ + H2O
2-oxoglutarate + NADH + H+
-
-
-
-
r
2,5-dioxopentanoate + NADP+ + H2O

2-oxoglutarate + NADPH + 2 H+
-
-
-
?
2,5-dioxopentanoate + NADP+ + H2O
2-oxoglutarate + NADPH + 2 H+
the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway
-
-
?
2,5-dioxopentanoate + NADP+ + H2O
2-oxoglutarate + NADPH + 2 H+
the enzyme is specific for NADP+
-
-
?
2,5-dioxopentanoate + NADP+ + H2O
2-oxoglutarate + NADPH + 2 H+
the enzyme is specific for NADP+
-
-
?
2,5-dioxopentanoate + NADP+ + H2O
2-oxoglutarate + NADPH + 2 H+
-
-
-
?
2,5-dioxopentanoate + NADP+ + H2O
2-oxoglutarate + NADPH + 2 H+
the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway
-
-
?
3-hydroxypropionaldehyde + NAD+

3-hydroxypropionate + NADH + H+
-
-
-
?
3-hydroxypropionaldehyde + NAD+
3-hydroxypropionate + NADH + H+
-
-
-
-
?
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O

alpha-ketoglutarate + NAD(P)H + H+
-
-
-
?
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O
alpha-ketoglutarate + NAD(P)H + H+
-
-
-
?
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O
alpha-ketoglutarate + NAD(P)H + H+
-
-
-
?
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O
alpha-ketoglutarate + NAD(P)H + H+
-
-
-
-
?
DL-glyceraldehyde + NADP+ + H2O

glycerate + NADPH + H+
-
-
-
?
DL-glyceraldehyde + NADP+ + H2O
glycerate + NADPH + H+
the enzyme is specific for NADP+
-
-
?
DL-glyceraldehyde + NADP+ + H2O
glycerate + NADPH + H+
the enzyme is specific for NADP+
-
-
?
DL-glyceraldehyde + NADP+ + H2O
glycerate + NADPH + H+
-
-
-
?
glycolaldehyde + NADP+ + H2O

glycolate + NADPH + H+
-
-
-
?
glycolaldehyde + NADP+ + H2O
glycolate + NADPH + H+
the enzyme is specific for NADP+
-
-
?
glycolaldehyde + NADP+ + H2O
glycolate + NADPH + H+
the enzyme is specific for NADP+
-
-
?
glycolaldehyde + NADP+ + H2O
glycolate + NADPH + H+
-
-
-
?
succinate semialdehyde + NAD+ + H2O

succinate + NADH + H+
activity of EC 1.2.1.24
-
-
?
succinate semialdehyde + NAD+ + H2O
succinate + NADH + H+
-
activity of EC 1.2.1.24
-
-
?
additional information

?
-
substrate and cofactor binding structures analysis, overview. The enzyme shows poor activity with NADP+ as a cofactor
-
-
-
additional information
?
-
-
substrate and cofactor binding structures analysis, overview. The enzyme shows poor activity with NADP+ as a cofactor
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2,5-dioxopentanoate + NAD(P)+ + H2O
2-oxoglutarate + NAD(P)H
2,5-dioxopentanoate + NADP+ + H2O
2-oxoglutarate + NADPH + 2 H+
2-oxoglutaric semialdehyde + NAD(P)+ + H2O
2-oxoglutarate + NAD(P)H + H+
-
-
-
?
2-oxoglutaric semialdehyde + NAD+ + H2O
2-oxoglutarate + NADH + H+
-
-
-
?
succinate semialdehyde + NAD+ + H2O
succinate + NADH + H+
2,5-dioxopentanoate + NAD(P)+ + H2O

2-oxoglutarate + NAD(P)H
-
-
-
?
2,5-dioxopentanoate + NAD(P)+ + H2O
2-oxoglutarate + NAD(P)H
-
-
-
?
2,5-dioxopentanoate + NAD(P)+ + H2O
2-oxoglutarate + NAD(P)H
-
-
-
?
2,5-dioxopentanoate + NAD(P)+ + H2O
2-oxoglutarate + NAD(P)H
-
-
-
?
2,5-dioxopentanoate + NAD(P)+ + H2O
2-oxoglutarate + NAD(P)H
-
-
-
?
2,5-dioxopentanoate + NAD(P)+ + H2O
2-oxoglutarate + NAD(P)H
-
-
-
?
2,5-dioxopentanoate + NAD(P)+ + H2O
2-oxoglutarate + NAD(P)H
-
-
-
?
2,5-dioxopentanoate + NAD(P)+ + H2O
2-oxoglutarate + NAD(P)H
-
-
-
?
2,5-dioxopentanoate + NADP+ + H2O

2-oxoglutarate + NADPH + 2 H+
the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway
-
-
?
2,5-dioxopentanoate + NADP+ + H2O
2-oxoglutarate + NADPH + 2 H+
the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway
-
-
?
succinate semialdehyde + NAD+ + H2O

succinate + NADH + H+
activity of EC 1.2.1.24
-
-
?
succinate semialdehyde + NAD+ + H2O
succinate + NADH + H+
-
activity of EC 1.2.1.24
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NAD+

-
-
NAD+
no significant preference between NAD+ and NADP+ in activity
NAD+
prefered cofactor of KGSADH-II
NAD+
NAD+-enzyme binding mode and structure, overview. The NAD+-binding pocket is constituted by seven loops (beta7-alpha4, beta8-alpha5, beta10-alpha7, beta11-beta12, alpha8-beta13, alpha9-alpha10, and alpha11-beta16) and four alpha-helices (alpha4, alpha6, alpha7, and alpha10). The adenine ring is stabilized in the hydrophobic pocket that is formed by Phe151, Pro211, Ala212, Phe229, Val235 and Leu239, and a hydrogen bond with Ser215 also contributes to the binding of the ring. Residues Lys178, Glu181, and Pro211 constitute a suitable space for binding of the ribose ring, and stabilize the 2'-hydroxyl-group of the ring. The formation of the ribose ring binding site does not seem to be large enough to accommodate the phosphorylated ribose ring. Therefore the enzyme shows poor activity with NADP+ as a cofactor. The diphosphate moiety is stabilized by residues Asn331, Arg333, and Arg334 through directly and water-mediated hydrogen bond networks. Residues Arg334 and Glu384 stabilize the ribose moiety of NAD+, and the nicotinamide ring is stabilized by residues Gln160 and Glu253 by hydrogen bonding
NAD+
preferred cofactor, residues chosen for generating the NAD+ binding pocket library are shown using the crystal structure of KGSADH complexed with NAD+ (PDB ID 5X5U), overview
NADP+

-
-
NADP+
alpha-KGSA dehydrogenase is an NADP+-preferring enzyme, since it is 58times more efficient with NADP+ than with NAD+
NADP+
prefered cofactor of KGSADH-III
NADP+
the enzyme is specific for NADP+
NADP+
low activity with 3-HPA
NADP+
the enzyme shows poor activity with NADP+ as a cofactor
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.05 - 0.1
2,5-dioxopentanoate
-
-
0.17 - 2.2
3-hydroxypropionaldehyde
3.9
acetaldehyde
with NADP+ as cofactor
0.0065 - 0.5569
alpha-Ketoglutaric semialdehyde
0.1 - 0.31
Glutaraldehyde
0.016
Glutaric semialdehyde
-
-
0.37
glycolaldehyde
pH 6.0, 70°C, native enzyme form xylose-grown cells
0.45 - 1.66
succinate semialdehyde
0.17
3-hydroxypropionaldehyde

pH 8.0, 25°C, recombinant mutant K273A/R334Q/A337R/A442P/T443E/P444A
0.29
3-hydroxypropionaldehyde
pH 8.0, 25°C, recombinant mutant R334Q/A337R/A442P/P444T
0.43
3-hydroxypropionaldehyde
pH 8.0, 25°C, recombinant mutant A110S/K273A/R334Q/A337R/A442P/P444T
0.55
3-hydroxypropionaldehyde
pH 8.0, 25°C, recombinant mutant R334Q/A337R
0.78
3-hydroxypropionaldehyde
pH 8.0, 25°C, recombinant mutant K273A/A442P/T443E/P444A
1.4
3-hydroxypropionaldehyde
pH 8.0, 25°C, recombinant mutant A442P/P444T
1.6
3-hydroxypropionaldehyde
pH 8.0, 25°C, recombinant wild-type enzyme
2.2
3-hydroxypropionaldehyde
pH 8.0, 25°C, recombinant mutant A110S/K273A/A442P/P444T
0.0065
alpha-Ketoglutaric semialdehyde

with NAD+ as cofactor
0.0075
alpha-Ketoglutaric semialdehyde
with NADP+ as cofactor
0.011
alpha-Ketoglutaric semialdehyde
with NADP+ as cofactor
0.014
alpha-Ketoglutaric semialdehyde
-
0.0246
alpha-Ketoglutaric semialdehyde
with NAD+ as cofactor
0.094
alpha-Ketoglutaric semialdehyde
-
with NADP+ as cofactor
0.5569
alpha-Ketoglutaric semialdehyde
-
with NAD+ as cofactor
0.1
Glutaraldehyde

with NADP+ as cofactor
0.31
Glutaraldehyde
with NAD+ as cofactor
0.025
NAD+

pH 8.0, 25°C, recombinant mutant R334Q/A337R/A442P/P444T
0.033
NAD+
pH 8.0, 25°C, recombinant mutant A110S/K273A/R334Q/A337R/A442P/P444T
0.037
NAD+
pH 8.0, 25°C, recombinant mutant K273A/R334Q/A337R/A442P/T443E/P444A
0.044
NAD+
pH 8.0, 25°C, recombinant mutant R334Q/A337R
0.21
NAD+
pH 8.0, 25°C, recombinant wild-type enzyme
0.27
NAD+
pH 8.0, 25°C, recombinant mutant A442P/P444T
0.27
NAD+
pH 8.0, 25°C, recombinant mutant K273A/A442P/T443E/P444A
0.28
NAD+
pH 8.0, 25°C, recombinant mutant A110S/K273A/A442P/P444T
0.0149
NADP+

-
0.54
NADP+
pH 8.0, 25°C, recombinant mutant R334Q/A337R/A442P/P444T
0.66
NADP+
pH 8.0, 25°C, recombinant mutant K273A/R334Q/A337R/A442P/T443E/P444A
0.7
NADP+
pH 8.0, 25°C, recombinant mutant A110S/K273A/R334Q/A337R/A442P/P444T
0.81
NADP+
pH 8.0, 25°C, recombinant mutant R334Q/A337R
2.3
NADP+
pH 8.0, 25°C, recombinant wild-type enzyme
0.3
propanal

with NADP+ as cofactor
1.05
propanal
with NAD+ as cofactor
0.45
succinate semialdehyde

with NAD+ as cofactor
1.66
succinate semialdehyde
with NADP+ as cofactor
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
8.6
2,5-dioxopentanoate
pH 7.5, 70°C
5 - 15
3-hydroxypropionaldehyde
0.41
acetaldehyde
with NADP+ as cofactor
1.12 - 197
alpha-Ketoglutaric semialdehyde
4.8
DL-glyceraldehyde
pH 7.5, 70°C
0.65 - 4.13
Glutaraldehyde
5.3
glycolaldehyde
pH 7.5, 70°C
0.05 - 0.06
succinate semialdehyde
5
3-hydroxypropionaldehyde

pH 8.0, 25°C, recombinant mutant R334Q/A337R/A442P/P444T
6
3-hydroxypropionaldehyde
pH 8.0, 25°C, recombinant mutant K273A/R334Q/A337R/A442P/T443E/P444A
6.7
3-hydroxypropionaldehyde
pH 8.0, 25°C, recombinant mutant A110S/K273A/R334Q/A337R/A442P/P444T
6.8
3-hydroxypropionaldehyde
pH 8.0, 25°C, recombinant mutant R334Q/A337R
11
3-hydroxypropionaldehyde
pH 8.0, 25°C, recombinant mutant K273A/A442P/T443E/P444A
12
3-hydroxypropionaldehyde
pH 8.0, 25°C, recombinant mutant A442P/P444T
15
3-hydroxypropionaldehyde
pH 8.0, 25°C, recombinant wild-type enzyme
15
3-hydroxypropionaldehyde
pH 8.0, 25°C, recombinant mutant A110S/K273A/A442P/P444T
1.12
alpha-Ketoglutaric semialdehyde

-
with NAD+ as cofactor
2.75
alpha-Ketoglutaric semialdehyde
-
with NADP+ as cofactor
15.4
alpha-Ketoglutaric semialdehyde
with NADP+ as cofactor
21.3
alpha-Ketoglutaric semialdehyde
with NAD+ as cofactor
23
alpha-Ketoglutaric semialdehyde
with NADP+ as cofactor
53.9
alpha-Ketoglutaric semialdehyde
with NAD+ as cofactor
197
alpha-Ketoglutaric semialdehyde
-
0.65
Glutaraldehyde

with NADP+ as cofactor
4.13
Glutaraldehyde
with NAD+ as cofactor
0.74
NAD+

-
-
4.8
NAD+
pH 8.0, 25°C, recombinant mutant R334Q/A337R/A442P/P444T
5.9
NAD+
pH 8.0, 25°C, recombinant mutant A110S/K273A/R334Q/A337R/A442P/P444T
6.1
NAD+
pH 8.0, 25°C, recombinant mutant K273A/R334Q/A337R/A442P/T443E/P444A
7.9
NAD+
pH 8.0, 25°C, recombinant mutant R334Q/A337R