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1alpha,23,25-trihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,25-dihydroxyvitamin D3-26,23-lactone + NADP+ + H2O
1alpha,24(R)-dihydroxyvitamin D3 + NADPH + H+ + O2
?
1alpha,24R,25-trihydroxyvitamin D3 + NADPH + O2
calcitroic acid + NADP+ + ?
1alpha,25-dihydroxy-3-epi-vitamin D3 + NADPH + H+ + O2
?
-
-
-
-
?
1alpha,25-dihydroxy-3-epivitamin-D3 + NADPH + H+ + O2
?
-
-
-
-
?
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,23,25-trihydroxyvitamin D3 + 25,26,27-trinor-23-ene-1alpha-hydroxyvitamin D3 + NADP+ + H2O
-
after hydrogen abstraction of the C-23 position of 1alpha,25-dihydroxyvitamin D3, part of the substrate-radical intermediate is converted into 25,26,27-trinor-23-ene-1alpha-hydroxyvitamin D3, while a major part of them is converted into 1alpha,23,25-trihydroxyvitamin D3
-
-
?
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,23,25-trihydroxyvitamin D3 + NADP+ + H2O
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,23S,25,26-tetrahydroxyvitamin D3 + 1alpha,23S,25-trihydroxyvitamin D3 + 25,26,27-trinor-23-ene-1alpha-hydroxyvitamin D3 + NADP+ + H2O
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,24(R)-dihydroxyvitamin D3 + NADP+ + H2O
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,24,25-trihydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,24R,25-trihydroxyvitamin D3 + NADP+ + H2O
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,25-dihydroxyvitamin D3-26,23-lactone + NADP+ + H2O
-
C23-hydroxylation
-
-
?
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
24-oxo-1,23,25-trihydroxyvitamin D3 + 24,25,26,27-tetranor-1,23-dihydroxyvitamin D3 + 1alpha,24R,25-trihydroxyvitamin D3 + 24-oxo-1,25-dihydroxyvitamin D3 + NADP+ + H2O
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
calcitroic acid + NADP+ + ?
1alpha-hydroxyvitamin D2 + NADPH + H+ + O2
1alpha,24-dihydroxyvitamin D2 + 1alpha,25-dihydroxyvitamin D2 + 26-carboxy-1alpha,24-dihydroxyvitamin D2 + NADP+ + H2O
1beta,25-dihydroxy-3-epi-vitamin D3 + NADPH + H+ + O2
?
-
-
-
-
?
1beta,25-dihydroxy-3-epivitamin-D3 + NADPH + H+ + O2
?
-
-
-
-
?
1beta,25-dihydroxyvitamin D3 + NADPH + H+ + O2
? + NADP+ + H2O
2-methylene-19-nor-(20R)-1,25-dihydroxyvitamin D3 + NADPH + H+ + O2
? + NADP+ + H2O
-
-
-
-
?
2-methylene-19-nor-(20S)-1,25-dihydroxyvitamin D3 + NADPH + H+ + O2
? + NADP+ + H2O
-
-
-
-
?
23-oxo-24,25,26,27-tetranor-1alpha-hydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
calcitroic acid + 2 oxidized adrenodoxin + H2O
24,25,26,27-tetranor-1alpha,23-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
23-oxo-24,25,26,27-tetranor-1alpha-hydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
24-oxo-1alpha,23,25-trihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
24,25,26,27-tetranor-1alpha,23-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
24-oxo-1alpha,23,25-trihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
25-hydroxyvitamin D3 + NADPH + H+ + O2
1alpha,25-dihydroxyvitamin D3-26,23-lactone + NADP+ + H2O
25-hydroxyvitamin D3 + NADPH + H+ + O2
24,25,26,27-tetranor-23-hydroxyvitamin-D3 + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D3 + NADPH + H+ + O2
24,25-dihydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D3 + NADPH + H+ + O2
24-oxo-25-hydroxyvitamin D3 + 23(S),25-dihydroxyvitamin D3 + 24(R),25-dihydroxyvitamin D3 + 24-oxo-23(S),25-dihydoxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D3 + NADPH + H+ + O2
24R,25-dihydroxyvitamin D3 + NADP+ + H2O
25-hydroxyvitamin D3 + NADPH + H+ + O2
25,26,27-trinor-23-ene-vitamin D3 + NADP+ + H2O
-
-
the metabolite 25,26,27-trinor-23-ene-vitamin D3 is closely related with the C-23 hydroxylation pathway
-
?
25-hydroxyvitamin D3 + NADPH + H+ + O2
secalciferol + NADP+ + H2O
-
-
-
-
?
3-epi-1alpha,23,25-trihydroxy-24-oxo-vitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
3-epi-1alpha,23-dihydroxy-24,25,26,27-tetranor-vitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
3-epi-1alpha,23,25-trihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
3-epi-1alpha,23,26-trihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
3-epi-1alpha,23,25-trihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
3-epi-1alpha,25-dihydroxy-23-oxovitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
3-epi-1alpha,23-dihydroxy-24,25,26,27-tetranor-vitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
3-epicalcitroic acid + 2 oxidized adrenodoxin + H2O
-
-
-
?
3-epi-1alpha,24,25-trihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
3-epi-1alpha,25-dihydroxy-24-oxo-vitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
3-epi-1alpha,25-dihydroxy-24-oxo-vitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
3-epi-1alpha,23,25-trihydroxy-24-oxo-vitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
3-epi-1alpha,25-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
3-epi-1alpha,23,25-trihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
3-epi-1alpha,25-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
3-epi-1alpha,24,25-trihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
3-epi-1alpha,25-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
3-epi-25,26,27-trinor-23-ene-1alpha-hydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
3-epi-25,26,27-trinor-23-ene-1alpha-hydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
3-epi-25,26,27-trinor-23,24-epoxide-1alpha-hydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2
secalciferol + 2 oxidized adrenodoxin + H2O
calcidiol + NADPH + H+ + O2
secalciferol + NADP+ + H2O
calcitetrol + 2 reduced adrenodoxin + 2 H+ + O2
24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
calcitriol + 2 reduced adrenodoxin + 2 H+ + O2
calcitetrol + 2 oxidized adrenodoxin + H2O
calcitriol + NADPH + H+ + O2
calcitetrol + NADP+ + H2O
additional information
?
-
1alpha,23,25-trihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,25-dihydroxyvitamin D3-26,23-lactone + NADP+ + H2O
-
-
-
-
?
1alpha,23,25-trihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,25-dihydroxyvitamin D3-26,23-lactone + NADP+ + H2O
-
-
-
-
?
1alpha,24(R)-dihydroxyvitamin D3 + NADPH + H+ + O2
?
-
i.e. tacalcitol, hydroxylation occurs at C-25
-
-
?
1alpha,24(R)-dihydroxyvitamin D3 + NADPH + H+ + O2
?
-
i.e. tacalcitol, hydroxylation occurs at C-25
-
-
?
1alpha,24R,25-trihydroxyvitamin D3 + NADPH + O2
calcitroic acid + NADP+ + ?
-
-
-
-
?
1alpha,24R,25-trihydroxyvitamin D3 + NADPH + O2
calcitroic acid + NADP+ + ?
-
-
-
-
?
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,23,25-trihydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,23,25-trihydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,23S,25,26-tetrahydroxyvitamin D3 + 1alpha,23S,25-trihydroxyvitamin D3 + 25,26,27-trinor-23-ene-1alpha-hydroxyvitamin D3 + NADP+ + H2O
-
the C-23 oxidation pathway
-
-
?
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,23S,25,26-tetrahydroxyvitamin D3 + 1alpha,23S,25-trihydroxyvitamin D3 + 25,26,27-trinor-23-ene-1alpha-hydroxyvitamin D3 + NADP+ + H2O
-
the C-23 oxidation pathway, which is almost not shown by rat CYP24A1
-
-
?
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,24(R)-dihydroxyvitamin D3 + NADP+ + H2O
-
1alpha,25-dihydroxyvitamin D3 is calcitriol
-
-
?
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,24(R)-dihydroxyvitamin D3 + NADP+ + H2O
-
1alpha,25-dihydroxyvitamin D3 is calcitriol
-
-
?
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,24R,25-trihydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,24R,25-trihydroxyvitamin D3 + NADP+ + H2O
-
C24-hydroxylation, the preferred pathway of human CYP24A1
-
-
?
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,24R,25-trihydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,24R,25-trihydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
24-oxo-1,23,25-trihydroxyvitamin D3 + 24,25,26,27-tetranor-1,23-dihydroxyvitamin D3 + 1alpha,24R,25-trihydroxyvitamin D3 + 24-oxo-1,25-dihydroxyvitamin D3 + NADP+ + H2O
-
the C-24 oxidation pathway
-
-
?
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
24-oxo-1,23,25-trihydroxyvitamin D3 + 24,25,26,27-tetranor-1,23-dihydroxyvitamin D3 + 1alpha,24R,25-trihydroxyvitamin D3 + 24-oxo-1,25-dihydroxyvitamin D3 + NADP+ + H2O
-
the C-24 oxidation pathway
-
-
?
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
calcitroic acid + NADP+ + ?
-
-
-
-
?
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
calcitroic acid + NADP+ + ?
-
-
-
-
?
1alpha-hydroxyvitamin D2 + NADPH + H+ + O2
1alpha,24-dihydroxyvitamin D2 + 1alpha,25-dihydroxyvitamin D2 + 26-carboxy-1alpha,24-dihydroxyvitamin D2 + NADP+ + H2O
-
-
-
-
?
1alpha-hydroxyvitamin D2 + NADPH + H+ + O2
1alpha,24-dihydroxyvitamin D2 + 1alpha,25-dihydroxyvitamin D2 + 26-carboxy-1alpha,24-dihydroxyvitamin D2 + NADP+ + H2O
-
-
-
-
?
1beta,25-dihydroxyvitamin D3 + NADPH + H+ + O2
? + NADP+ + H2O
-
-
-
-
?
1beta,25-dihydroxyvitamin D3 + NADPH + H+ + O2
? + NADP+ + H2O
-
-
-
-
?
23-oxo-24,25,26,27-tetranor-1alpha-hydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
calcitroic acid + 2 oxidized adrenodoxin + H2O
-
-
-
?
23-oxo-24,25,26,27-tetranor-1alpha-hydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
calcitroic acid + 2 oxidized adrenodoxin + H2O
-
-
-
?
24,25,26,27-tetranor-1alpha,23-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
23-oxo-24,25,26,27-tetranor-1alpha-hydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
24,25,26,27-tetranor-1alpha,23-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
23-oxo-24,25,26,27-tetranor-1alpha-hydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
24-oxo-1alpha,23,25-trihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
24,25,26,27-tetranor-1alpha,23-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
24-oxo-1alpha,23,25-trihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
24,25,26,27-tetranor-1alpha,23-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
24-oxo-1alpha,23,25-trihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
24-oxo-1alpha,23,25-trihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
25-hydroxyvitamin D3 + NADPH + H+ + O2
1alpha,25-dihydroxyvitamin D3-26,23-lactone + NADP+ + H2O
-
C-23 hydroxylation
-
-
?
25-hydroxyvitamin D3 + NADPH + H+ + O2
1alpha,25-dihydroxyvitamin D3-26,23-lactone + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D3 + NADPH + H+ + O2
24R,25-dihydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D3 + NADPH + H+ + O2
24R,25-dihydroxyvitamin D3 + NADP+ + H2O
-
C-24 hydroxylation
-
-
?
25-hydroxyvitamin D3 + NADPH + H+ + O2
24R,25-dihydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D3 + NADPH + H+ + O2
24R,25-dihydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2
secalciferol + 2 oxidized adrenodoxin + H2O
-
-
-
?
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2
secalciferol + 2 oxidized adrenodoxin + H2O
-
-
-
-
?
calcidiol + NADPH + H+ + O2
secalciferol + NADP+ + H2O
-
-
-
-
?
calcidiol + NADPH + H+ + O2
secalciferol + NADP+ + H2O
-
-
-
-
?
calcitetrol + 2 reduced adrenodoxin + 2 H+ + O2
24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
calcitetrol + 2 reduced adrenodoxin + 2 H+ + O2
24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
-
?
calcitetrol + 2 reduced adrenodoxin + 2 H+ + O2
24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
calcitriol + 2 reduced adrenodoxin + 2 H+ + O2
calcitetrol + 2 oxidized adrenodoxin + H2O
-
-
-
?
calcitriol + 2 reduced adrenodoxin + 2 H+ + O2
calcitetrol + 2 oxidized adrenodoxin + H2O
-
-
-
-
?
calcitriol + 2 reduced adrenodoxin + 2 H+ + O2
calcitetrol + 2 oxidized adrenodoxin + H2O
-
-
-
-
?
calcitriol + 2 reduced adrenodoxin + 2 H+ + O2
calcitetrol + 2 oxidized adrenodoxin + H2O
-
-
-
?
calcitriol + 2 reduced adrenodoxin + 2 H+ + O2
calcitetrol + 2 oxidized adrenodoxin + H2O
-
recombinant truncated DELTA108 adrenodoxin from Bos taurus is used
-
-
?
calcitriol + NADPH + H+ + O2
calcitetrol + NADP+ + H2O
-
-
-
-
?
calcitriol + NADPH + H+ + O2
calcitetrol + NADP+ + H2O
-
the h-P450cc24 activity is approximately 10fold higher for calcitriol than for calcidiol
-
-
?
calcitriol + NADPH + H+ + O2
calcitetrol + NADP+ + H2O
-
-
-
-
?
calcitriol + NADPH + H+ + O2
calcitetrol + NADP+ + H2O
-
-
-
-
?
additional information
?
-
-
25-hydroxyvitamin D3-24-hydroxylase (CYP24A1) is a bifunctional enzyme capable of the 24-hydroxylation of 1alpha,25-dihydroxyvitamin D3, leading to the excretory form, calcitroic acid (calcitetrol), and 23-hydroxylation, culminating in 1alpha,25-dihydroxyvitamin D3-26,23-lactone
-
-
?
additional information
?
-
-
human CYP24A1 catalyzes both C-23 and C-24 oxidation pathways of 1alpha,25-dihydroxyvitamin D3
-
-
?
additional information
?
-
-
no activity with 1alpha-hydroxyvitamin D3
-
-
?
additional information
?
-
calcidiol, the precursor to calcitriol, is also a substrate of CYP24A1 and is metabolized through similar pathways
-
-
?
additional information
?
-
CYP24A1 also catalyzes the 23-hydroxylase pathway that degrades calcidiol and calcitriol
-
-
?
additional information
?
-
-
CYP24A1 also catalyzes the 23-hydroxylase pathway that degrades calcidiol and calcitriol
-
-
?
additional information
?
-
enzyme activity measurements with reconstituted enzyme in phospholipid vesicles
-
-
?
additional information
?
-
to mediate catalysis, mitochondrial P450 enzymes, including CYP27B1 and CYP24A1, must receive electrons fromreduced nicotinamide adenine dinucleotide phosphate via ferredoxin reductase and ferredoxin. The mammalian two-hybrid assay system consisting of the retinoid-X receptor and vitamin D receptor in JEG-3 cells is used as a simple, sensitive, quantitative assay of enzyme activity
-
-
?
additional information
?
-
-
to mediate catalysis, mitochondrial P450 enzymes, including CYP27B1 and CYP24A1, must receive electrons fromreduced nicotinamide adenine dinucleotide phosphate via ferredoxin reductase and ferredoxin. The mammalian two-hybrid assay system consisting of the retinoid-X receptor and vitamin D receptor in JEG-3 cells is used as a simple, sensitive, quantitative assay of enzyme activity
-
-
?
additional information
?
-
isolation and identification of 3-epi-calcitroic acid as the final inactive metabolite of 3-epi-1alpha,25-dihydroxyvitamin D3 produced by rat CYP24A1. Metabolite determination, overview
-
-
?
additional information
?
-
the precursor to calcitriol, calcidiol, is also a substrate of CYP24A1 and is metabolized through similar pathways
-
-
?
additional information
?
-
-
detailed analysis of the reversible complex between recombinant truncated enzyme DELT51CYP24A1 and recombinant truncated DELTA108adrenodoxin, kinetics, overview. Adrenodoxin binding enhances the stability of the enzyme-substrate complex, despite lowering the ligand binding affinity of the free enzyme for calcitriol over 9fold
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,24,25-trihydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
1alpha,24R,25-trihydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
1alpha,25-dihydroxyvitamin D3 + NADPH + H+ + O2
calcitroic acid + NADP+ + ?
-
-
-
-
?
23-oxo-24,25,26,27-tetranor-1alpha-hydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
calcitroic acid + 2 oxidized adrenodoxin + H2O
24,25,26,27-tetranor-1alpha,23-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
23-oxo-24,25,26,27-tetranor-1alpha-hydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
24-oxo-1alpha,23,25-trihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
24,25,26,27-tetranor-1alpha,23-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
24-oxo-1alpha,23,25-trihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
25-hydroxyvitamin D3 + NADPH + H+ + O2
1alpha,25-dihydroxyvitamin D3-26,23-lactone + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D3 + NADPH + H+ + O2
24,25-dihydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
3-epi-1alpha,23,25-trihydroxy-24-oxo-vitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
3-epi-1alpha,23-dihydroxy-24,25,26,27-tetranor-vitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
3-epi-1alpha,23,25-trihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
3-epi-1alpha,23,26-trihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
3-epi-1alpha,23,25-trihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
3-epi-1alpha,25-dihydroxy-23-oxovitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
3-epi-1alpha,23-dihydroxy-24,25,26,27-tetranor-vitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
3-epicalcitroic acid + 2 oxidized adrenodoxin + H2O
-
-
-
?
3-epi-1alpha,24,25-trihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
3-epi-1alpha,25-dihydroxy-24-oxo-vitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
3-epi-1alpha,25-dihydroxy-24-oxo-vitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
3-epi-1alpha,23,25-trihydroxy-24-oxo-vitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
3-epi-1alpha,25-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
3-epi-1alpha,23,25-trihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
3-epi-1alpha,25-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
3-epi-1alpha,24,25-trihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
3-epi-1alpha,25-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
3-epi-25,26,27-trinor-23-ene-1alpha-hydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
3-epi-25,26,27-trinor-23-ene-1alpha-hydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
3-epi-25,26,27-trinor-23,24-epoxide-1alpha-hydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2
secalciferol + 2 oxidized adrenodoxin + H2O
calcidiol + NADPH + H+ + O2
secalciferol + NADP+ + H2O
-
-
-
-
?
calcitetrol + 2 reduced adrenodoxin + 2 H+ + O2
24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
calcitriol + 2 reduced adrenodoxin + 2 H+ + O2
calcitetrol + 2 oxidized adrenodoxin + H2O
additional information
?
-
23-oxo-24,25,26,27-tetranor-1alpha-hydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
calcitroic acid + 2 oxidized adrenodoxin + H2O
-
-
-
?
23-oxo-24,25,26,27-tetranor-1alpha-hydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
calcitroic acid + 2 oxidized adrenodoxin + H2O
-
-
-
?
24,25,26,27-tetranor-1alpha,23-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
23-oxo-24,25,26,27-tetranor-1alpha-hydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
24,25,26,27-tetranor-1alpha,23-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
23-oxo-24,25,26,27-tetranor-1alpha-hydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
24-oxo-1alpha,23,25-trihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
24,25,26,27-tetranor-1alpha,23-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
24-oxo-1alpha,23,25-trihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
24,25,26,27-tetranor-1alpha,23-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
24-oxo-1alpha,23,25-trihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 reduced adrenodoxin + 2 H+ + O2
24-oxo-1alpha,23,25-trihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2
secalciferol + 2 oxidized adrenodoxin + H2O
-
-
-
?
calcidiol + 2 reduced adrenodoxin + 2 H+ + O2
secalciferol + 2 oxidized adrenodoxin + H2O
-
-
-
-
?
calcitetrol + 2 reduced adrenodoxin + 2 H+ + O2
24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
calcitetrol + 2 reduced adrenodoxin + 2 H+ + O2
24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
-
?
calcitetrol + 2 reduced adrenodoxin + 2 H+ + O2
24-oxo-1alpha,25-dihydroxyvitamin D3 + 2 oxidized adrenodoxin + H2O
-
-
-
?
calcitriol + 2 reduced adrenodoxin + 2 H+ + O2
calcitetrol + 2 oxidized adrenodoxin + H2O
-
-
-
?
calcitriol + 2 reduced adrenodoxin + 2 H+ + O2
calcitetrol + 2 oxidized adrenodoxin + H2O
-
-
-
-
?
calcitriol + 2 reduced adrenodoxin + 2 H+ + O2
calcitetrol + 2 oxidized adrenodoxin + H2O
-
-
-
-
?
calcitriol + 2 reduced adrenodoxin + 2 H+ + O2
calcitetrol + 2 oxidized adrenodoxin + H2O
-
-
-
?
additional information
?
-
-
no activity with 1alpha-hydroxyvitamin D3
-
-
?
additional information
?
-
calcidiol, the precursor to calcitriol, is also a substrate of CYP24A1 and is metabolized through similar pathways
-
-
?
additional information
?
-
CYP24A1 also catalyzes the 23-hydroxylase pathway that degrades calcidiol and calcitriol
-
-
?
additional information
?
-
-
CYP24A1 also catalyzes the 23-hydroxylase pathway that degrades calcidiol and calcitriol
-
-
?
additional information
?
-
isolation and identification of 3-epi-calcitroic acid as the final inactive metabolite of 3-epi-1alpha,25-dihydroxyvitamin D3 produced by rat CYP24A1. Metabolite determination, overview
-
-
?
additional information
?
-
the precursor to calcitriol, calcidiol, is also a substrate of CYP24A1 and is metabolized through similar pathways
-
-
?
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