Information on EC 1.14.14.69 - ent-cassadiene hydroxylase

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The expected taxonomic range for this enzyme is: Oryza sativa Japonica Group

EC NUMBER
COMMENTARY hide
1.14.14.69
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RECOMMENDED NAME
GeneOntology No.
ent-cassadiene hydroxylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ent-cassa-12,15-dien-2-one + [reduced NADPH-hemoprotein reductase] + O2 = ent-3beta-hydroxycassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
(1c)
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ent-cassa-12,15-dien-2beta-ol + [reduced NADPH-hemoprotein reductase] + O2 = ent-cassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O
show the reaction diagram
(1b)
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ent-cassa-12,15-dien-2beta-ol + [reduced NADPH-hemoprotein reductase] + O2 = ent-cassa-12,15-diene-2beta,3beta-diol + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
(1b')
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ent-cassa-12,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 = ent-3beta-hydroxycassa-12,15-dien-2-one + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
show the reaction diagram
overall reaction
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ent-cassa-12,15-diene + [reduced NADPH-hemoprotein reductase] + O2 = ent-cassa-12,15-dien-2beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
(1a)
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ent-cassa-12,15-diene-2beta,3beta-diol + [reduced NADPH-hemoprotein reductase] + O2 = ent-3beta-hydroxycassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O
show the reaction diagram
(1c')
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Diterpenoid biosynthesis
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Biosynthesis of secondary metabolites
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SYSTEMATIC NAME
IUBMB Comments
ent-cassa-12,15-diene,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (ent-3?-hydroxycassa-12,15-dien-2-one forming)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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a Cyp71Z7 T-DNA mutant accumulates potential phytoalexin intermediates such as 1-deoxyphytocassane C and 2-deoxyphytocassane A. In planta, Cyp71Z7 is responsible for C2-hydroxylation of phytocassanes
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ent-cassa-12,15-dien-2-one + [reduced NADPH-hemoprotein reductase] + O2
ent-3beta-hydroxycassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
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?
ent-cassa-12,15-dien-2beta-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-cassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O
show the reaction diagram
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-
?
ent-cassa-12,15-dien-2beta-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-cassa-12,15-diene-2beta,3beta-diol + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
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?
ent-cassa-12,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-3beta-hydroxycassa-12,15-dien-2-one + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
show the reaction diagram
ent-cassa-12,15-diene + [reduced NADPH-hemoprotein reductase] + O2
ent-cassa-12,15-dien-2beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
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?
ent-cassa-12,15-diene-2beta,3beta-diol + [reduced NADPH-hemoprotein reductase] + O2
ent-3beta-hydroxycassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O
show the reaction diagram
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?
additional information
?
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enzyme catalyzes the production of 3alpha-hydroxy-ent-cassadien-2-one via 2alpha-hydroxy-ent-cassadiene and ent-cassadien-2-one
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2
ent-cassa-12,15-diene
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pH not specified in the publication, temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.11
ent-cassa-12,15-diene
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pH not specified in the publication, temperature not specified in the publication
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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