We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 1.14.14.69 - ent-cassadiene hydroxylase for references in articles please use BRENDA:EC1.14.14.69Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
The expected taxonomic range for this enzyme is: Oryza sativa Japonica Group
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ent-cassadiene hydroxylase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ent-cassa-12,15-dien-2-one + [reduced NADPH-hemoprotein reductase] + O2 = ent-3beta-hydroxycassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
(1c)
-
-
-
ent-cassa-12,15-dien-2beta-ol + [reduced NADPH-hemoprotein reductase] + O2 = ent-cassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O
(1b)
-
-
-
ent-cassa-12,15-dien-2beta-ol + [reduced NADPH-hemoprotein reductase] + O2 = ent-cassa-12,15-diene-2beta,3beta-diol + [oxidized NADPH-hemoprotein reductase] + H2O
(1b')
-
-
-
ent-cassa-12,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 = ent-3beta-hydroxycassa-12,15-dien-2-one + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
overall reaction
-
-
-
ent-cassa-12,15-diene + [reduced NADPH-hemoprotein reductase] + O2 = ent-cassa-12,15-dien-2beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
(1a)
-
-
-
ent-cassa-12,15-diene-2beta,3beta-diol + [reduced NADPH-hemoprotein reductase] + O2 = ent-3beta-hydroxycassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O
(1c')
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Diterpenoid biosynthesis
-
-
Biosynthesis of secondary metabolites
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ent-cassa-12,15-diene,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (ent-3?-hydroxycassa-12,15-dien-2-one forming)
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ent-cassadiene C2-hydroxylase
-
CYP71Z7
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
physiological function
-
a Cyp71Z7 T-DNA mutant accumulates potential phytoalexin intermediates such as 1-deoxyphytocassane C and 2-deoxyphytocassane A. In planta, Cyp71Z7 is responsible for C2-hydroxylation of phytocassanes
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ent-cassa-12,15-dien-2-one + [reduced NADPH-hemoprotein reductase] + O2
ent-3beta-hydroxycassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-cassa-12,15-dien-2beta-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-cassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
ent-cassa-12,15-dien-2beta-ol + [reduced NADPH-hemoprotein reductase] + O2
ent-cassa-12,15-diene-2beta,3beta-diol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-cassa-12,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-3beta-hydroxycassa-12,15-dien-2-one + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
ent-cassa-12,15-diene + [reduced NADPH-hemoprotein reductase] + O2
ent-cassa-12,15-dien-2beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-cassa-12,15-diene-2beta,3beta-diol + [reduced NADPH-hemoprotein reductase] + O2
ent-3beta-hydroxycassa-12,15-dien-2-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
additional information
?
-
enzyme catalyzes the production of 3alpha-hydroxy-ent-cassadien-2-one via 2alpha-hydroxy-ent-cassadiene and ent-cassadien-2-one
-
-
-
ent-cassa-12,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-3beta-hydroxycassa-12,15-dien-2-one + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
ent-cassa-12,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
ent-3beta-hydroxycassa-12,15-dien-2-one + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.2
ent-cassa-12,15-diene
-
pH not specified in the publication, temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.11
ent-cassa-12,15-diene
-
pH not specified in the publication, temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expression in Escherichia coli
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
C71Z7_ORYSJ
518
57416
Swiss-Prot
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Kitaoka, N.; Wu, Y.; Xu, M.; Peters, R.J.
Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis
Appl. Microbiol. Biotechnol.
99
7549-7558
2015
Oryza sativa Japonica Group (Q6YV88)
brenda
Ye, Z.; Yamazaki, K.; Minoda, H.; Miyamoto, K.; Miyazaki, S.; Kawaide, H.; Yajima, A.; Nojiri, H.; Yamane, H.; Okada, K.
In planta functions of cytochrome P450 monooxygenase genes in the phytocassane biosynthetic gene cluster on rice chromosome 2
Biosci. Biotechnol. Biochem.
69
1021-1030
2018
Oryza sativa Japonica Group (Q6YV88)
brenda
Wu, Y.; Hillwig, M.L.; Wang, Q.; Peters, R.J.
Parsing a multifunctional biosynthetic gene cluster from rice Biochemical characterization of CYP71Z6 & 7
FEBS Lett.
585
3446-3451
2011
Oryza sativa Japonica Group (Q6YV88)
brenda
html completed