Information on EC 1.14.14.68 - syn-pimaradiene 3-monooxygenase

for references in articles please use BRENDA:EC1.14.14.68
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The expected taxonomic range for this enzyme is: Oryza sativa

EC NUMBER
COMMENTARY hide
1.14.14.68
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RECOMMENDED NAME
GeneOntology No.
syn-pimaradiene 3-monooxygenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
9beta-pimara-7,15-diene + [reduced NADPH-hemoprotein reductase] + O2 = 9beta-pimara-7,15-diene-3beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Diterpenoid biosynthesis
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Biosynthesis of secondary metabolites
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SYSTEMATIC NAME
IUBMB Comments
9beta-pimara7,15-diene,[reduced NADPHbetahemoprotein reductase]:oxygen oxidoreductase (9beta-pimara-7,15-diene-3beta-ol forming)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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enzyme efficiently reacts with ent-sandaracopimaradiene and ent-cassadiene to produce the corresponding C3alpha-hydroxylated diterpenoids, reaction of EC 1.14.13.191, intermediates in biosynthesis of the oryzalexin and phytocassane families of rice antifungal phytoalexins
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
9beta-pimara-7,15-diene + [reduced NADPH-hemoprotein reductase] + O2
9beta-pimara-7,15-diene-3beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.027
9beta-pimara-7,15-diene
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pH 7.5, 32°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.4
9beta-pimara-7,15-diene
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pH 7.5, 32°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
52
9beta-pimara-7,15-diene
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pH 7.5, 32°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32
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assay at