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IUBMB CommentsA cytochrome P450 (heme thiolate) enzyme found in plants. The enzyme acts on jasmonoyl-L-amino acid conjugates that have been hydroxylated at the C-12 position of jasmonic acid by EC 1.14.14.48, jasmonoyl-L-amino acid 12-hydroxylase, further oxidizing that position to a carboxylate via an aldehyde intermediate. While the best studied substrate is (+)-7-epi-jasmonoyl-L-isoleucine, the enzyme was shown to be active with jasmonoyl-L-phenylalanine, and is likely to be active with other jasmonoyl-amino acid conjugates.
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12-hydroxy-dodecanoic acid + reduced NADPH-hemoprotein reductase + O2
12-oxo-dodecanoic acid + oxidized NADPH-hemoprotein reductase + H2O
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12-hydroxyjasmonoyl-L-isoleucine + reduced NADPH-hemoprotein reductase + O2
12-hydroxy-12-oxojasmonoyl-L-isoleucine + oxidized NADPH-hemoprotein reductase + H2O
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12-hydroxyjasmonoyl-L-isoleucine + [reduced NADPH-hemoprotein reductase] + O2
12-hydroxy-12-oxojasmonoyl-L-isoleucine + [oxidized NADPH-hemoprotein reductase] + H2O
12-hydroxylauric acid + reduced NADPH-hemoprotein reductase + O2
12-hydroxy-dodecanoic acid + oxidized NADPH-hemoprotein reductase + H2O
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12-oxo-dodecanoic acid + reduced NADPH-hemoprotein reductase + O2
dodecadioic acid + oxidized NADPH-hemoprotein reductase + H2O
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jasmonoyl-L-isoleucine + [reduced NADPH-hemoprotein reductase] + O2
12-hydroxyjasmonoyl-L-isoleucine + [oxidized NADPH-hemoprotein reductase] + H2O
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12-hydroxyjasmonoyl-L-isoleucine + [reduced NADPH-hemoprotein reductase] + O2
12-hydroxy-12-oxojasmonoyl-L-isoleucine + [oxidized NADPH-hemoprotein reductase] + H2O
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-
-
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?
12-hydroxyjasmonoyl-L-isoleucine + [reduced NADPH-hemoprotein reductase] + O2
12-hydroxy-12-oxojasmonoyl-L-isoleucine + [oxidized NADPH-hemoprotein reductase] + H2O
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-
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?
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Poudel, A.N.; Zhang, T.; Kwasniewski, M.; Nakabayashi, R.; Saito, K.; Koo, A.J.
Mutations in jasmonoyl-L-isoleucine-12-hydroxylases suppress multiple JA-dependent wound responses in Arabidopsis thaliana
Biochim. Biophys. Acta
1861
1396-1408
2016
Arabidopsis thaliana (Q9ZUX1)
brenda
Kandel, S.; Sauveplane, V.; Compagnon, V.; Franke, R.; Millet, Y.; Schreiber, L.; Werck-Reichhart, D.; Pinot, F.
Characterization of a methyl jasmonate and wounding-responsive cytochrome P450 of Arabidopsis thaliana catalyzing dicarboxylic fatty acid formation in vitro
FEBS J.
274
5116-5127
2007
Arabidopsis thaliana (Q9ZUX1)
brenda
Heitz, T.; Widemann, E.; Lugan, R.; Miesch, L.; Ullmann, P.; Desaubry, L.; Holder, E.; Grausem, B.; Kandel, S.; Miesch, M.; Werck-Reichhart, D.; Pinot, F.
Cytochromes P450 CYP94C1 and CYP94B3 catalyze two successive oxidation steps of plant hormone jasmonoyl-isoleucine for catabolic turnover
J. Biol. Chem.
287
6296-6306
2012
Arabidopsis thaliana
brenda
Koo, A.J.; Thireault, C.; Zemelis, S.; Poudel, A.N.; Zhang, T.; Kitaoka, N.; Brandizzi, F.; Matsuura, H.; Howe, G.A.
Endoplasmic reticulum-associated inactivation of the hormone jasmonoyl-L-isoleucine by multiple members of the cytochrome P450 94 family in Arabidopsis
J. Biol. Chem.
289
29728-29738
2014
Arabidopsis thaliana (Q9ZUX1)
brenda
Aubert, Y.; Widemann, E.; Miesch, L.; Pinot, F.; Heitz, T.
CYP94-mediated jasmonoyl-isoleucine hormone oxidation shapes jasmonate profiles and attenuates defence responses to Botrytis cinerea infection
J. Exp. Bot.
66
3879-3892
2015
Arabidopsis thaliana (Q9ZUX1)
brenda
Widemann, E.; Grausem, B.; Renault, H.; Pineau, E.; Heinrich, C.; Lugan, R.; Ullmann, P.; Miesch, L.; Aubert, Y.; Miesch, M.; Heitz, T.; Pinot, F.
Sequential oxidation of jasmonoyl-phenylalanine and jasmonoyl-isoleucine by multiple cytochrome P450 of the CYP94 family through newly identified aldehyde intermediates
Phytochemistry
117
388-399
2015
Arabidopsis thaliana (Q9ZUX1)
brenda
Bruckhoff, V.; Haroth, S.; Feussner, K.; Koenig, S.; Brodhun, F.; Feussner, I.
Functional characterization of CYP94-genes and identification of a novel jasmonate catabolite in flowers
PLoS ONE
11
e0159875
2016
Arabidopsis thaliana (Q9ZUX1)
brenda