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Information on EC 1.14.14.153 - indole-2-monooxygenase for references in articles please use BRENDA:EC1.14.14.153
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IUBMB Comments A cytochrome P-450 (heme-thiolate) protein. The enzyme is involved in the biosynthesis of protective and allelophatic benzoxazinoids in some plants, most commonly from the family of Poaceae (grasses).
The expected taxonomic range for this enzyme is: Zea mays
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BX2
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gene name
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indole + [reduced NADPH-hemoprotein reductase] + O2 = indolin-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
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indole,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (2-hydroxylating)
A cytochrome P-450 (heme-thiolate) protein. The enzyme is involved in the biosynthesis of protective and allelophatic benzoxazinoids in some plants, most commonly from the family of Poaceae (grasses).
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indole + [reduced NADPH-hemoprotein reductase] + O2
indolin-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
indole + [reduced NADPH-hemoprotein reductase] + O2
indolin-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
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indole + [reduced NADPH-hemoprotein reductase] + O2
indolin-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
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cytochrome P450
dependent on
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0.036
indole
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native enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C
0.038
indole
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recombinant enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C
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0.00033
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recombinant enzyme, using indole as substrate, at pH 7.5 and 25°C
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hybrid Blizzard
UniProt
brenda
hybrid Blizzard
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brenda
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brenda
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C71C4_MAIZE
538
1
60048
Swiss-Prot
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expressed in Saccharomyces cerevisiae strain WAT11 and in Escherichia coli strain BL21 (DE3)
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expressed in Saccharomyces cerevisiae WAT11 cells
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Frey, M.; Chomet, P.; Glawischnig, E.; Stettner, C.; Grun, S.; Winklmair, A.; Eisenreich, W.; Bacher, A.; Meeley, R.B.; Briggs, S.P.; Simcox, K.; Gierl, A.
Analysis of a chemical plant defense mechanism in grasses
Science
277
696-699
1997
Zea mays (Q43257)
brenda
Glawischnig, E.; Grün, S.; Frey, M.; Gierl, A.
Cytochrome P450 monooxygenases of DIBOA biosynthesis: Specificity and conservation among grasses
Phytochemistry
50
925-930
1999
Zea mays
brenda
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