Information on EC 1.14.13.79 - ent-kaurenoic acid oxidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.13.79
transferred to EC 1.14.14.107
RECOMMENDED NAME
GeneOntology No.
ent-kaurenoic acid oxidase
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Diterpenoid biosynthesis
-
-
Metabolic pathways
-
-
Biosynthesis of secondary metabolites
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cv. Himalaya
Uniprot
Manually annotated by BRENDA team
cv. Fuji
UniProt
Manually annotated by BRENDA team
cv. Dongjinbye
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
ent-kaurenoic acid oxidase (KAO) belongs to a class of cytochrome P450 monooxygenases of the subfamily CYP88A; ent-kaurenoic acid oxidase (KAO) belongs to a class of cytochrome P450 monooxygenases of the subfamily CYP88A
malfunction
metabolism
the enzyme is important in the gibberellin biosynthesis, pathway overview; the enzyme is important in the gibberellin biosynthesis, pathway overview
physiological function
additional information
OsWOX3A acts as a transcriptional repressor, generation of transgenic rice overexpressing OsWOX3A, OsWOX3A-OX, the mutant consistently exhibits severe dwarfism with very short and wide leaves, a phenotype that resembles that of gibberellic acid (GA)-deficient or GA-insensitive mutants. OsWOX3A expression is drastically and temporarily upregulated by gibberellin GA3 and is downregulated by paclobutrazol, a blocker of gibberellic acid biosynthesis
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ent-kaur-16-en-19-oate + 3 NADPH + 3 H+ + 3 O2
gibberellin A12 + 3 NADP+ + 4 H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ent-kaur-16-en-19-oate + 3 NADPH + 3 H+ + 3 O2
gibberellin A12 + 3 NADP+ + 4 H2O
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
-
NADPH
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene HvKAO1, quantitative RT-PCR enzyme expression analysis
gene KAO encoding ent-kaurenoic acid oxidase, quantitative RT-PCR enzyme expression analysis
gene KAO1, phylogenetic analysis and tree; gene KAO2, phylogenetic analysis and tree
gene MdKAO, DNA and amino acid sequence determination and analysis, the sequence contains 7 introns, location on chromosome 2, quantitative real-time PCR enzyme expression analysis
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
diphenyleneiodonium reduces the expression of HvKAO1, about 20% at 1 mM
exogenous H2O2 promotes the expression of HvKAO1 in barley embryos
OsWOX3A interacts directly with the KAO promoter to repress KAO expression. OsWOX3A interacts with a WOX-binding motif, TTAATCG, in the KAO promoter, mechanism modeling, overview. OsWOX3A is involved in the negative feedback regulation of KAO expression for gibberellic acid homeostasis
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information