The enzyme, characterized from Pseudomonas putida strains, catalyses the first step in a pathway for degradation of the plant hormone indole-3-acetate. When acting on indole, the enzyme forms indoxyl, which reacts spontaneously with oxygen to form the blue dye indigo.
strain 1290 is able to to use indole-3-acetic acid as a sole source of carbon, nitrogen, and energy. In root elongation assays, strain 1290 completely abolishes the inhibitory effect of exogenous indole-3-acetic acid on the elongation of radish roots
enzyme catalyzes the first step in degradation of the plant hormone indole-3-acetate. Transformation of Pseudomonas putida KT2440, which cannot degrade IAA, with the Iac gene cluster confers the ability to grow on indole-3-acetate as a sole source of carbon and energy, but not the ability to chemotaxis towards indole-3-acetate
enzyme catalyzes the first step in degradation of the plant hormone indole-3-acetate. Transformation of Pseudomonas putida KT2440, which cannot degrade IAA, with the Iac gene cluster confers the ability to grow on indole-3-acetate as a sole source of carbon and energy, but not the ability to chemotaxis towards indole-3-acetate; strain 1290 is able to to use indole-3-acetic acid as a sole source of carbon, nitrogen, and energy. In root elongation assays, strain 1290 completely abolishes the inhibitory effect of exogenous indole-3-acetic acid on the elongation of radish roots
coinoculation of roots with strain 1290 and 1 mM of indole-3-acetic acid has a positive effect on root development. In coinoculation experiments on radish roots, strain 1290 is partially able to alleviate the inhibitory effect of bacteria that in culture overproduce indole-3-acetic acid
coinoculation of roots with strain 1290 and 1 mM of indole-3-acetic acid has a positive effect on root development. In coinoculation experiments on radish roots, strain 1290 is partially able to alleviate the inhibitory effect of bacteria that in culture overproduce indole-3-acetic acid