Information on EC 1.14.13.235 - indole-3-acetate monooxygenase

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The expected taxonomic range for this enzyme is: Pseudomonas putida

EC NUMBER
COMMENTARY hide
1.14.13.235
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RECOMMENDED NAME
GeneOntology No.
indole-3-acetate monooxygenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(indol-3-yl)acetate + NADH + H+ + O2 = (2-hydroxy-1H-indol-3-yl)acetate + NAD+ + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
indole-3-acetate degradation
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SYSTEMATIC NAME
IUBMB Comments
(indol-3-yl)acetate,NADH:oxygen oxidoreductase (2-hydroxylating)
The enzyme, characterized from Pseudomonas putida strains, catalyses the first step in a pathway for degradation of the plant hormone indole-3-acetate. When acting on indole, the enzyme forms indoxyl, which reacts spontaneously with oxygen to form the blue dye indigo.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(indol-3-yl)acetate + NADH + H+ + O2
(2-hydroxyindol-3-yl)acetate + NAD+ + H2O
show the reaction diagram
additional information
?
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
with indole-3-acetate as the sole source of carbon and energy, the expression of IacA is more than 2 orders of magnitude greater than with glucose
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture