Information on EC 1.14.13.202 - methyl farnesoate epoxidase

for references in articles please use BRENDA:EC1.14.13.202
Word Map on EC 1.14.13.202
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.13.202
transferred to EC 1.14.14.127
RECOMMENDED NAME
GeneOntology No.
methyl farnesoate epoxidase
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Insect hormone biosynthesis
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
D6CJJ9
UniProt
Manually annotated by BRENDA team
collected from colonies kept in the Meliponary of the University of Uberlandia, MG Brazil
A0A286LD89
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
-
the methyl farnesoate epoxidase gene (MFE) is an indicator of juvenile hormone production. Virgin queens are distinguished by higher expression of forkhead box protein O and downregulated insulin-like peptides and juvenile hormone signaling, indicated by low expression of methyl farnesoate epoxidase (MFE) and transcription factor Krüppel homolog 1 (Kr-h1), while reproducing queens reveal an upregulation of MFE and vitellogenin together with insulin signaling. Workers exhibit an expression pattern of MFE and vitellogenin similar to that of reproducing queens. Males aere characterized by high Kr-h1expression and low vitellogenin level
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
methyl (2E,6E)-farnesoate + NADPH + H+ + O2
juvenile hormone III + NADP+ + H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
methyl (2E,6E)-farnesoate + NADPH + H+ + O2
juvenile hormone III + NADP+ + H2O
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
-
NADPH
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.29
sequence calculation
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
A0A286LD89;
-
Manually annotated by BRENDA team
expression level of EcMFE is significantly high in the head of males
Manually annotated by BRENDA team
A0A286LD89;
-
Manually annotated by BRENDA team
-
of workers and reproducing queens, high MFE expression in ovaries of bumblebee females
Manually annotated by BRENDA team
A0A286LD89;
-
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
the enzyme has a membrane anchor region at amino acids 2-19, the transmembrane region is located at amino acids 141-158
Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 54810, sequence calculation
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene CYP15A1, RT-PCR enzyme expression analysis
D6CJJ9;
gene EcMFE, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis
gene mfe, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis
A0A286LD89;
gene MFE, quantitative RT-PCR enzyme expression analysis
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
starving reduces the enzyme expression
D6CJJ9;
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
EcMFE RNAi-mediated knockdown. In EcMFE dsRNA treatment, there are no significant differences between the treated group and controls until the 5th day after injection, when compared on cantharidin content, the cantharidin content is significantly reduced on the 7th day after injection