Information on EC 1.14.13.188 - 6-deoxyerythronolide B hydroxylase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.13.188
transferred to EC 1.14.15.35
RECOMMENDED NAME
GeneOntology No.
6-deoxyerythronolide B hydroxylase
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of 12-, 14- and 16-membered macrolides
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Biosynthesis of antibiotics
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
halophilic actinomycete isolated from Baicheng salt field
UniProt
Manually annotated by BRENDA team
halophilic actinomycete isolated from Baicheng salt field
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
6-deoxyerythronolide B + NADPH + H+ + O2
erythronolide B + NADP+ + H2O
show the reaction diagram
6-deoxyerythronolide B + NADPH + H+ + O2
erythronolide H + NADP+ + H2O
show the reaction diagram
a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B
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-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6-deoxyerythronolide B + NADPH + H+ + O2
erythronolide B + NADP+ + H2O
show the reaction diagram
6-deoxyerythronolide B + NADPH + H+ + O2
erythronolide H + NADP+ + H2O
show the reaction diagram
A0A099D4T8
a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B
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-
?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
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Ferredoxin
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NADPH
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene eryF, genetic structure, sequence comparison, recombinant expression of His6-tagged enzyme in Saccharopolyspora erythraea strain ZL2001
gene eryF, whole-genome sequencing, DNA and amino acid sequence determination and analysis, genetic structure,sequence comparison, genomic DNA of Saccharopolyspora erythraea strain ZL2001 serves as template for PCR amplification, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), recombinant expression in and complementation fo Saccharopolyspora erythraea eryF-deletion mutant strain EX105(ZL2001)
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information