Information on EC 1.14.13.184 - protopanaxadiol 6-hydroxylase

for references in articles please use BRENDA:EC1.14.13.184
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The expected taxonomic range for this enzyme is: Panax

EC NUMBER
COMMENTARY hide
1.14.13.184
transferred to EC 1.14.14.121
RECOMMENDED NAME
GeneOntology No.
protopanaxadiol 6-hydroxylase
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
protopanaxatriol synthase, which is considered cytochrome P450 (CYP450) in ginsenoside biosynthesis pathway can convert protopanaxadiol into protopanaxatriol
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
protopanaxadiol + NADPH + H+ + O2
protopanaxatriol + NADP+ + H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
protopanaxadiol + NADPH + H+ + O2
protopanaxatriol + NADP+ + H2O
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
-
NADPH
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene CYP6H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, RT-PCR expression analysis, functional recombinant ectopic expression of gene CYP6H in Saccharomyces cerevisiae results in the production of protopanaxatriol with added exogenous protopanaxadiol, mass spectrometric analysis, recombinant overexpression in Panax quinquefolius hairy roots from pBI121-CYP6H-overexpression plasmid
gene CYP716A53v2, recombinant overexpression in Panx ginseng roots using the Agrobacterium tumefaciens strain GV3101 transformation method, expression under control of CaMV 35S promoter, subcloning in Escherichia coli strain DH5alpha
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the gene CYP6H shows no obvious transcription change in methyl jasmonate (MeJA)-induced hairy roots
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information