two cytochrome P450 (CYP) genes, CYP716A47 and CYP716A53v2, are critical for the production of protopanaxadiol and protopanaxatriol aglycones, respectively. CYP716A53v2 is a protopanaxadiol 6-hydroxylase that catalyzes protopanaxatriol production from protopanaxadiol in Panax ginseng, biosynthetic pathway for ginsenosides in Panax ginseng, overview
construction of transgenic Panax ginseng lines overexpressing CYP716A53v2 gene or silencing CYP716A53v2 gene via RNA interference (RNAi), analyis changes in ginsenoside profiles, overview. Production of protopanaxadiol- and protopanaxatriol-group ginsenosides can be altered by changing the expression of CYP716A53v2 in transgenic Panax ginseng. Changing the expression of CYP716A53v2 affects the downstream genes (glycosyltransferases)
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gene CYP6H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, RT-PCR expression analysis, functional recombinant ectopic expression of gene CYP6H in Saccharomyces cerevisiae results in the production of protopanaxatriol with added exogenous protopanaxadiol, mass spectrometric analysis, recombinant overexpression in Panax quinquefolius hairy roots from pBI121-CYP6H-overexpression plasmid
gene CYP716A53v2, recombinant overexpression in Panx ginseng roots using the Agrobacterium tumefaciens strain GV3101 transformation method, expression under control of CaMV 35S promoter, subcloning in Escherichia coli strain DH5alpha