Information on EC 1.14.13.173 - 11-oxo-beta-amyrin 30-oxidase

for references in articles please use BRENDA:EC1.14.13.173
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The expected taxonomic range for this enzyme is: Platycodon grandiflorus

EC NUMBER
COMMENTARY hide
1.14.13.173
transferred to EC 1.14.14.115
RECOMMENDED NAME
GeneOntology No.
11-oxo-beta-amyrin 30-oxidase
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the enzyme belongs to the CYP716A subfamily of cytochrome P450 monooxygenases
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta-amyrin + NADPH + O2
maniladiol + NADP+ + H2O
show the reaction diagram
beta-amyrin + NADPH + O2
oleanolic acid + NADP+ + H2O
show the reaction diagram
maniladiol + NADPH + O2
cochalic acid + NADP+ + H2O
show the reaction diagram
hydroxylation at C-28
-
-
?
oleanolic acid + NADPH + O2
cochalic acid + NADP+ + H2O
show the reaction diagram
hydroxylation at C-16beta
-
-
?
additional information
-
-
CYP716A141 is capable of catalyzing oxidation at both C-16beta and C-28, metabolites analysis by mass spectrometry
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
beta-amyrin + NADPH + O2
maniladiol + NADP+ + H2O
show the reaction diagram
A0A1I9Q5Z0
hydroxylation at C-16beta
-
-
?
beta-amyrin + NADPH + O2
oleanolic acid + NADP+ + H2O
show the reaction diagram
A0A1I9Q5Z0
hydroxylation at C-28
-
-
?
maniladiol + NADPH + O2
cochalic acid + NADP+ + H2O
show the reaction diagram
A0A1I9Q5Z0
hydroxylation at C-28
-
-
?
oleanolic acid + NADPH + O2
cochalic acid + NADP+ + H2O
show the reaction diagram
A0A1I9Q5Z0
hydroxylation at C-16beta
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
the enzyme is highly expressed in roots
Manually annotated by BRENDA team
additional information
transcriptome analysis in different tissues using RNA sequencing technology, overview
Manually annotated by BRENDA team
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene CYP716A141, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR enzyme expression analysis, recombinant coexpression of genes CYP716A140v2 and CYP716A141 in Saccharomyces cerevisiae strain 10, that shows increased cochalic acid levels