We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 1.14.13.161 - (+)-camphor 6-exo-hydroxylase for references in articles please use BRENDA:EC1.14.13.161Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(+)-camphor 6-exo-hydroxylase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(+)-camphor + NADPH + H+ + O2 = (+)-6-exo-hydroxycamphor + NADP+ + H2O
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(+)-camphor,NADPH:oxygen oxidoreductase (6-exo-hydroxylating)
A cytochrome P-450 monooxygenase isolated from Salvia officinalis (sage). Involved in the catabolism of camphor in senescent tissue.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(+)-camphor 6-hydroxylase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
physiological function
-
pathway for camphor degradation follows the oxidative ring opening sequence from (+)-camphor to 6-hydroxycamphor, 6-oxocamphor, alpha-campholonic acid, and 2-hydroxy-alpha-campholonic acid. The catabolism of camphor via 1,2-campholide is a minor degradative pathway
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(+)-camphor + NADPH + H+ + O2
(+)-6-exo-hydroxycamphor + NADP+ + H2O
limonene + NADPH + H+ + O2
(-)-perillyl alcohol + NADP+ + H2O
-
-
-
-
-
additional information
?
-
-
the microsomal camphor-6-exo-hydroxylase system requires molecular oxygen and a reduced pyridine nucleotide
-
-
-
(+)-camphor + NADPH + H+ + O2
(+)-6-exo-hydroxycamphor + NADP+ + H2O
-
-
-
-
-
(+)-camphor + NADPH + H+ + O2
(+)-6-exo-hydroxycamphor + NADP+ + H2O
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cytochrome P450
-
enzyme is a cytochrome P450-dependent monooxygenase
-
NADH
-
21% of the rate with NADPH
NADPH
-
absolute requirement for NADPH and O2
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MnCl2
-
upon treatment of sage suspension cultures with 30 mM MnCI2, camphor-6-hydroxylase activity is induced up to 7fold
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
clotrimazole
-
84% inhibition at 0.05 mM
CO
-
73% inhibition at CO:O2 ratio of 9:1, activity is partially restored by blue light
cytochrome c
-
95% inhibition at 0.05 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
FAD
-
inclusion of both FAD and FMN in the incubation mixture enhances hydroxylation activity 2.4fold
FMN
-
inclusion of both FAD and FMN in the incubation mixture enhances hydroxylation activity 2.4fold
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.034
(+)-Camphor
-
pH 7.0, 32°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6.5 - 7.5
-
more than 60% of maximum activity within
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
microsomal membrane
-
brenda
-
light membrane fraction
brenda
-
microsomal membrane
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
58000
-
x * 58000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expression in Escherichia coli
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
the genes responsible for early steps of D-camphor degradation, i.e. 5-exo-hydroxycamphor dehydrogenase, camD gene, cytochrome P-450cam, camC, NADH-putidaredoxin reductase camA, and putidaredoxin camB form an operon, camDCAB, and are under negative control by the gene camR located immediately upstream from the camD gene
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
functional expression in Escherichia coli of tricistronic constructs consisting of P450cam and the auxiliary proteins, Pd and PdR. Transformed bacterial whole cells efficiently oxidize (1R)-(+)-camphor to 6-exo-hydroxycamphor and interestingly limonene to (-)-perillyl alcohol. These bioengineered Escherichia coli cells possess a heterologous selfsufficient P450 catalytic system
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Kim, D.; Ortiz de Montellano, P.R.
Tricistronic overexpression of cytochrome P450cam, putidaredoxin, and putidaredoxin reductase provides a useful cell-based catalytic system
Biotechnol. Lett.
31
1427-1431
2009
Pseudomonas putida
brenda
Funk, C.; Koepp, A.E.; Croteau, R.
Catabolism of camphor in tissue cultures and leaf disks of common sage (Salvia officinalis)
Arch. Biochem. Biophys.
294
306-313
1992
Salvia officinalis
brenda
Koga, H.; Yamaguchi, E.; Matsunaga, K.; Aramaki, H.; Horiuchi, T.
Cloning and nucleotide sequences of NADH-putidaredoxin reductase gene (camA) and putidaredoxin gene (camB) involved in cytochrome P-450cam hydroxylase of Pseudomonas putida
J. Biochem.
106
831-836
1989
Pseudomonas putida
brenda
Funk, C.; Croteau, R.
Induction and characterization of a cytochrome P-450-dependent camphor hydroxylase in tissue cultures of common sage (Salvia officinalis)
Plant Physiol.
101
1231-1237
1993
Salvia officinalis
brenda
html completed