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EC Tree
IUBMB Comments Requires cytochrome P-450. From the yew tree Taxus cuspidata. Does not act on earlier intermediates in taxol biosynthesis.
The expected taxonomic range for this enzyme is: Taxus cuspidata
Synonyms
T7H, taxoid 7beta-hydroxylase,
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taxoid 7beta-hydroxylase
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taxoid 7beta-hydroxylase
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taxusin + O2 + NADPH + H+ = 7beta-hydroxytaxusin + NADP+ + H2O
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taxusin,NADPH:oxygen 7-oxidoreductase
Requires cytochrome P-450. From the yew tree Taxus cuspidata. Does not act on earlier intermediates in taxol biosynthesis.
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(+)-taxusin + O2 + NADPH + H+
7beta-hydroxytaxusin + NADP+ + H2O
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i.e. taxadien-5alpha,9alpha,10beta,13alpha-tetraol tetraacetate
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taxusin + O2 + NADPH + H+
7beta-hydroxytaxusin + NADP+ + H2O
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taxusin + O2 + NADPH + H+
7beta-hydroxytaxusin + NADP+ + H2O
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cytochrome P450
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NADPH
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0.0076
(+)-taxusin
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pH 7.5, temperature not specified in the publication
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UniProt
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T7H_TAXCU
500
2
56324
Swiss-Prot
other Location (Reliability: 5 )
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taxhydroxylase activity is slightly higher in HEPES than in Tris-HCl, however, hydrolytic activity (enzymatic deacylation of both substrate and product) is also more prominent in HEPES7
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expressed in a Spodoptera fugiperda-baculovirus-based expression system
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Chau, M.; Jennewein, S.; Walker, K.; Croteau, R.
Taxol biosynthesis: Molecular cloning and characterization of a cytochrome P450 taxoid 7beta-hydroxylase
Chem. Biol.
11
663-672
2004
Taxus cuspidata
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Kaspera, R.; Croteau, R.
Cytochrome P450 oxygenases of Taxol biosynthesis
Phytochem. Rev.
5
433-444
2006
Taxus cuspidata (Q6JTJ0)
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1.14.13.147 taxoid 7beta-hydroxylase
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