We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments Requires cytochrome P-450. From the yew tree Taxus cuspidata. Does not act on earlier intermediates in taxol biosynthesis.
The expected taxonomic range for this enzyme is: Taxus cuspidata
Synonyms
T7H, taxoid 7beta-hydroxylase,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
taxoid 7beta-hydroxylase
-
-
taxoid 7beta-hydroxylase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
taxusin + O2 + NADPH + H+ = 7beta-hydroxytaxusin + NADP+ + H2O
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
taxusin,NADPH:oxygen 7-oxidoreductase
Requires cytochrome P-450. From the yew tree Taxus cuspidata. Does not act on earlier intermediates in taxol biosynthesis.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(+)-taxusin + O2 + NADPH + H+
7beta-hydroxytaxusin + NADP+ + H2O
-
i.e. taxadien-5alpha,9alpha,10beta,13alpha-tetraol tetraacetate
-
-
?
taxusin + O2 + NADPH + H+
7beta-hydroxytaxusin + NADP+ + H2O
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
taxusin + O2 + NADPH + H+
7beta-hydroxytaxusin + NADP+ + H2O
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cytochrome P450
-
-
-
NADPH
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0076
(+)-taxusin
-
pH 7.5, temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
T7H_TAXCU
500
2
56324
Swiss-Prot
other Location (Reliability: 5 )
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
taxhydroxylase activity is slightly higher in HEPES than in Tris-HCl, however, hydrolytic activity (enzymatic deacylation of both substrate and product) is also more prominent in HEPES7
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in a Spodoptera fugiperda-baculovirus-based expression system
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Chau, M.; Jennewein, S.; Walker, K.; Croteau, R.
Taxol biosynthesis: Molecular cloning and characterization of a cytochrome P450 taxoid 7beta-hydroxylase
Chem. Biol.
11
663-672
2004
Taxus cuspidata
brenda
Kaspera, R.; Croteau, R.
Cytochrome P450 oxygenases of Taxol biosynthesis
Phytochem. Rev.
5
433-444
2006
Taxus cuspidata (Q6JTJ0)
brenda
Select items on the left to see more content.
html completed