Information on EC 1.14.13.147 - taxoid 7beta-hydroxylase

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The expected taxonomic range for this enzyme is: Taxus cuspidata

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EC NUMBER
COMMENTARY hide
1.14.13.147
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RECOMMENDED NAME
GeneOntology No.
taxoid 7beta-hydroxylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
taxusin + O2 + NADPH + H+ = 7beta-hydroxytaxusin + NADP+ + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
2alpha,7beta-dihydroxylation of taxusin
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SYSTEMATIC NAME
IUBMB Comments
taxusin,NADPH:oxygen 7-oxidoreductase
Requires cytochrome P-450. From the yew tree Taxus cuspidata. Does not act on earlier intermediates in taxol biosynthesis.
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-taxusin + O2 + NADPH + H+
7beta-hydroxytaxusin + NADP+ + H2O
show the reaction diagram
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i.e. taxadien-5alpha,9alpha,10beta,13alpha-tetraol tetraacetate
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?
taxusin + O2 + NADPH + H+
7beta-hydroxytaxusin + NADP+ + H2O
show the reaction diagram
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?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
taxusin + O2 + NADPH + H+
7beta-hydroxytaxusin + NADP+ + H2O
show the reaction diagram
Q6JTJ0
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?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0076
(+)-taxusin
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pH 7.5, temperature not specified in the publication
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
taxhydroxylase activity is slightly higher in HEPES than in Tris-HCl, however, hydrolytic activity (enzymatic deacylation of both substrate and product) is also more prominent in HEPES7
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in a Spodoptera fugiperda-baculovirus-based expression system
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LINKS TO OTHER DATABASES (specific for EC-Number 1.14.13.147)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)