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Information on EC 1.14.13.14 - trans-cinnamate 2-monooxygenase

for references in articles please use BRENDA:EC1.14.13.14

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EC Tree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.13.14 trans-cinnamate 2-monooxygenase

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The enzyme appears in viruses and cellular organisms

Reaction Schemes

Synonyms

cinnamate 2-hydroxylase, cinnamate 2-monooxygenase, cinnamic 2-hydroxylase, cinnamic acid 2-hydroxylase, trans-cinnamic acid 2-hydroxylase, show all synonyms

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SYNONYM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

cinnamate 2-hydroxylase

cinnamate 2-monooxygenase

cinnamic 2-hydroxylase

cinnamic acid 2-hydroxylase

trans-cinnamic acid 2-hydroxylase

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REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

trans-cinnamate + NADPH + H+ + O2 = 2-hydroxycinnamate + NADP+ + H2O

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REACTION TYPE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

oxidation

redox reaction

reduction

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PATHWAY SOURCE

PATHWAYS

KEGG

Biosynthesis of secondary metabolites, Microbial metabolism in diverse environments, Phenylalanine metabolism, Phenylpropanoid biosynthesis

MetaCyc

coumarin biosynthesis (via 2-coumarate)

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SYSTEMATIC NAME

IUBMB Comments

trans-cinnamate,NADPH:oxygen oxidoreductase (2-hydroxylating)

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CAS REGISTRY NUMBER

COMMENTARY

53126-56-0

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SUBSTRATE

PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

trans-cinnamate + NADPH + O2

2-hydroxycinnamate + NADP+ + H2O

2 entries

trans-cinnamate + NADPH + O2

2-hydroxycinnamate + NADP+ + H2O

Cucumis sativus

439037

trans-cinnamate + NADPH + O2

2-hydroxycinnamate + NADP+ + H2O

Melilotus albus

439038

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

trans-cinnamate + NADPH + O2

2-hydroxycinnamate + NADP+ + H2O

2 entries

trans-cinnamate + NADPH + O2

2-hydroxycinnamate + NADP+ + H2O

Cucumis sativus

439037

trans-cinnamate + NADPH + O2

2-hydroxycinnamate + NADP+ + H2O

Melilotus albus

439038

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COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

NADH

Melilotus albus

less effective than NADPH

NADPH

NADPH

NADPH

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

KCN

Cucumis sativus

70% inhibition at 10 mM

439037

Triton X-100

Cucumis sativus

concentration higher than 0.5% deactivates the enzyme

439037

additional information

Cucumis sativus

UV-light treatment for 200 min leads to 30% inhibition

439037

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ACTIVATING COMPOUND

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

glucose 6-phosphate

Melilotus albus

400% activation at 4 mM

439038

sulfonic acid

Cucumis sativus

700% activation at 0.003%

439037

Triton X-100

Cucumis sativus

700% activation at 0.025%

439037

additional information

Cucumis sativus

white light treatment for 200 min leads to 50% activation

439037

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0.0004

Melilotus albus

supernatant activity

439038

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pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Melilotus albus

439038

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

cucumber

brenda

brenda

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SOURCE TISSUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

SOURCE

brenda

brenda

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LOCALIZATION

ORGANISM

UNIPROT

COMMENTARY

GeneOntology No.

LITERATURE

SOURCE

chloroplast

Cucumis sativus

predominantly, chloroplast membrane

brenda

lamellar, 50% of the hydroxylase activity

16020

439038

brenda

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

439037

Czichi, U.; Kindl, H.

Phenylalanine ammonia lyase and cinnamic acid hydroxylases as assembled consecutive enzymes on microsomal membranes of cucumber cotyledons: cooperation and subcellular distribution

Planta

134

133-143

1977

Cucumis sativus

24419691

brenda

439038

Gestetner, B.; Conn, E.E.

The 2-hydroxylation of trans-cinnamic acid by chloroplasts from Melilotus alba Desr

Arch. Biochem. Biophys.

163