Information on EC 1.14.12.16 - 2-Hydroxyquinoline 5,6-dioxygenase

for references in articles please use BRENDA:EC1.14.12.16
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)


The expected taxonomic range for this enzyme is: Comamonas testosteroni

top print hide
EC NUMBER
COMMENTARY hide
1.14.12.16
-
top print hide
RECOMMENDED NAME
GeneOntology No.
2-Hydroxyquinoline 5,6-dioxygenase
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
quinolin-2-ol + NADH + H+ + O2 = 2,5,6-trihydroxy-5,6-dihydroquinoline + NAD+
show the reaction diagram
-
-
-
-
top print hide
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
reduction
-
-
-
-
top print hide
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
3-methylquinoline degradation
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
quinolin-2-ol,NADH:oxygen oxidoreductase (5,6-hydroxylating)
3-Methylquinolin-2-ol, quinolin-8-ol and quinolin-2,8-diol are also substrates. Quinolin-2-ols exist largely as their quinolin-2(1H)-one tautomers
top
top print hide
CAS REGISTRY NUMBER
COMMENTARY hide
172399-50-7
not distinguished from EC 1.14.13.65
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-Oxo-1,2-dihydroquinoline + NADH + O2
?
show the reaction diagram
3-Methyl-2-oxo-1,2-dihydroquinoline + NADH + O2
5,6-Dihydro-5,6-dihydroxy-(3-methyl-)2-oxo-1,2-dihydroquinoline + NAD+
show the reaction diagram
6-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2
?
show the reaction diagram
8-Hydroxy-2-oxo-1,2-dihydroquinoline + NADH + O2
?
show the reaction diagram
-
-
-
-
-
8-Hydroxyquinoline + NADH + O2
?
show the reaction diagram
additional information
?
-
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
top print hide
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
required, cannot be replaced by NADPH
top print hide
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
-
enhances activity 1.5fold
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
0.5 mM, 82% loss of activity
4-hydroxymercuribenzoate
-
0.1 mM, complete loss of activity
Acriflavin
-
0.5 mM, 69% loss of activity
diethyldithiocarbaminate
-
inhibits after a prolonged incubation time
-
EDTA
-
inhibits after a prolonged incubation time
iodoacetate
-
2 mM, complete loss of activity
NaCl
-
0.2 M, 50% loss of activity
Quinacrine
-
0.5 mM, 15% loss of activity
top print hide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
5 min, 30% loss of activity
65
-
5 min, complete loss of activity
top print hide
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dithioerythritol, dithiothreitol and 2-oxo-1,2-dihydroquinoline stabilize
-
top print hide
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ethanol
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 1.14.12.16)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)