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EC Tree
The expected taxonomic range for this enzyme is: Pantoea ananatis
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HilA
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L-isoleucine + 2-oxoglutarate + O2 = (3S)-3'-hydroxy-L-isoleucine + succinate + CO2
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L-isoleucine,2-oxoglutarate:oxygen oxidoreductase (3'-hydroxylating)
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L-isoleucine + 2-oxoglutarate + O2
(3S)-3'-hydroxy-L-isoleucine + succinate + CO2
L-isoleucine + 2-oxoglutarate + O2
(3S)-3'-hydroxy-L-isoleucine + succinate + CO2
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ir
L-isoleucine + 2-oxoglutarate + O2
(3S)-3'-hydroxy-L-isoleucine + succinate + CO2
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ir
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L-isoleucine + 2-oxoglutarate + O2
(3S)-3'-hydroxy-L-isoleucine + succinate + CO2
L-isoleucine + 2-oxoglutarate + O2
(3S)-3'-hydroxy-L-isoleucine + succinate + CO2
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ir
L-isoleucine + 2-oxoglutarate + O2
(3S)-3'-hydroxy-L-isoleucine + succinate + CO2
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ir
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brenda
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brenda
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Superdex 200 HR 10/30A column
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expressed in Escherichia coli BL21(DE3) cells
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Smirnov, S.; Sokolov, P.; Kotlyarova, V.; Samsonova, N.; Kodera, T.; Sugiyama, M.; Torii, T.; Hibi, M.; Shimizu, S.; Yokozeki, K.; Ogawa, J.
A novel L-isoleucine-4'-dioxygenase and L-isoleucine dihydroxylation cascade in Pantoea ananatis
MicrobiologyOpen
2
471-481
2013
Pantoea ananatis, Pantoea ananatis AJ13355
brenda
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1.14.11.B20 L-isoleucine 3'-hydroxylase
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