Information on EC 1.14.11.B18 - chalcone 3-hydroxylase

for references in articles please use BRENDA:EC1.14.11.B18
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The expected taxonomic range for this enzyme is: Dahlia pinnata

EC NUMBER
COMMENTARY hide
1.14.11.B18
-
RECOMMENDED NAME
GeneOntology No.
chalcone 3-hydroxylase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
isoliquiritigenin + 2-oxoglutarate + O2 = butein + succinate + CO2
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
chalcone 3-dioxygenase
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
D3IWE6 i.e. allele 2, enzyme additionally catalyzes the reaction of EC 1.14.14.82
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
isoliquiritigenin + 2-oxoglutarate + O2
butein + succinate + CO2
show the reaction diagram
hydroxylation in position 3, i.e. reaction of chalcone 3-hydroxylase
-
-
?
naringenin chalcone + 2-oxoglutarate + O2
eriodictyol chalcone
show the reaction diagram
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0039
isoliquiritigenin
pH 7.5, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
V425A
complete loss of the ability th hydroxylate isoliquiritigenin in position 3