Information on EC 1.14.11.B1 - [histone-H3]-lysine-9-demethylase

for references in articles please use BRENDA:EC1.14.11.B1
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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.11.B1
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
[histone-H3]-lysine-9-demethylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
protein N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2 = protein N6-methyl-L-lysine9 + succinate + formaldehyde + CO2
show the reaction diagram
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
gene PFF1440w
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATKAARK(me3)-SAPATGGVKKPHRYRPG-GK(biotin) + 2-oxoglutarate + O2
ATKAARKSAPATGGVKKPHRYRPG-GK(biotin) + succinate + formaldehyde + CO2
show the reaction diagram
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usage of immunodetection for assay quantification
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?
dimethyl-histone 3 L-lysine 9 + alpha-ketoglutarate + O2
methyl-histone 3 L-lysine 9 + ?
show the reaction diagram
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-
?
dimethyl-histone 3 lysine 9 + ?
methyl-histone 3 lysine 9 + ?
show the reaction diagram
dimethylated histone 3 lysine 9 + ?
methyl-histone 3 lysine 9 + ?
show the reaction diagram
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proteins Swm1 and Swm2 associate together in a complex that specifically demethylates lysine 9 in histone H3 and both up- and down-regulates expression of different groups of genes. Protein Swm1 may act in concert with the histone deacetylase Clr6 and chromatin remodeller Hrp1 to repress gene expression
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?
dimethylated histone 3-Lys4 peptide + H2O
?
show the reaction diagram
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the enzyme specifically removes methyl groups from Lys4 of histone 3. The enzyme exhibits oxidase activity (with production of H2O2) but it can function also with a synthetic mono-electronic acceptor
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?
H3(1-20) K4-dimethylated peptide + 2-oxoglutarate + O2
H3(1-20) K4-monomethylated peptide + succinate + formaldehyde + CO2
show the reaction diagram
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?
H31-15K9me3 + 2-oxoglutarate + O2
H31-15K9me2 + succinate + formaldehyde + CO2
show the reaction diagram
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a 15mer histone peptide substrate H31-15K9me3
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?
histone H3 N6,N6,N6-trimethyl-L-lysine9 + 2-oxoglutarate + O2
histone H3 N6,N6-dimethyl-L-lysine9 + succinate + formaldehyde + CO2
show the reaction diagram
histone H3 N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2
histone H3 N6-methyl-L-lysine9 + succinate + formaldehyde + CO2
show the reaction diagram
histone H3 N6-methyl-L-lysine9 + 2-oxoglutarate + O2
histone H3 L-lysine9 + succinate + formaldehyde + CO2
show the reaction diagram
histone H4 N6-methyl-L-lysine20 + 2-oxoglutarate + O2
histone H4 L-lysine20 + succinate + formaldehyde + CO2
show the reaction diagram
protein 6-N,6-N-dimethyl-L-lysine + 2-oxoglutarate + O2
?
show the reaction diagram
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demethylation of lysine 4
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?
protein N6,N6-dimethyl-L-lysine + 2 2-oxoglutarate + 2 O2
protein L-lysine + 2 succinate + 2 formaldehyde + 2 CO2
show the reaction diagram
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?
trimethyl-histone 3 L-lysine 9 + 2-oxoglutarate + O2
dimethyl-histone 3 L-lysine 9 + ?
show the reaction diagram
-
-
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?
trimethyl-histone 3 L-lysine 9 + alpha-ketoglutarate + O2
dimethyl-histone 3 L-lysine 9 + ?
show the reaction diagram
trimethyl-histone 3 L-lysine 9 mutant A7H + alpha-ketoglutarate + O2
dimethyl-histone 3 L-lysine 9 mutant A7H + ?
show the reaction diagram
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?
trimethyl-histone 3 L-lysine 9 mutant A7R + alpha-ketoglutarate + O2
dimethyl-histone 3 L-lysine 9 mutant A7R + ?
show the reaction diagram
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?
trimethyl-histone 3 L-lysine 9 mutant G12P + alpha-ketoglutarate + O2
dimethyl-histone 3 lysine 9 mutant G12P + ?
show the reaction diagram
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?
[Dnmt1]-methyl-L-lysine + 2-oxoglutarate + O2
[Dnmt1]-L-lysine + succinate + formaldehyde + CO2
show the reaction diagram
[Dnmt1]-methyl-L-lysine1096 + 2-oxoglutarate + O2
[Dnmt1]-L-lysine1096 + succinate + formaldehyde + CO2
show the reaction diagram
[histone H3 peptide 21mer P16A]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2
?
show the reaction diagram
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?
[histone H3 peptide 21mer]-N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2
[histone H3 peptide 21mer]-L-lysine4 + succinate + formaldehyde + CO2
show the reaction diagram
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?
[histone H3 peptide 21mer]-N6,N6-dimethyl-L-lysine4-dimethyl-L-lysine9 + 2-oxoglutarate + O2
?
show the reaction diagram
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?
[histone H3 peptide 21mer]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2
[histone H3 peptide 21mer]-L-lysine4 + succinate + formaldehyde + CO2
show the reaction diagram
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no activity with a monomethylated H3-Lys4 peptide with Arg2 mutated to Ala, Arg2 is central to substrate recognition also in LSD2
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?
[histone H3 peptide 21mer]-N6-methyl-L-lysine4-acetyl-L-lysine9 + 2-oxoglutarate + O2
[histone H3 peptide 21mer]-L-lysine4-acetyl-L-lysine9 + succinate + formaldehyde + CO2
show the reaction diagram
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?
[histone H3 peptide 21mer]-N6-methyl-L-lysine4-methyl-L-arginine17 + 2-oxoglutarate + O2
?
show the reaction diagram
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?
[histone H3 peptide 21mer]-N6-methyl-L-lysine4-methyl-L-lysine9 + 2-oxoglutarate + O2
?
show the reaction diagram
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?
[histone H3 peptide 30mer]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2
[histone H3 peptide 30mer]-L-lysine4 + succinate + formaldehyde + CO2
show the reaction diagram
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?
[histone H3 peptide]-N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2
[histone H3 peptide]-L-lysine4 + succinate + H2O2
show the reaction diagram
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the peptide substrate comprises the 21 N-terminal residues of histone H3, with a dimethylated lysyl residue at position 4. The reductive half-reaction is rate-limiting at physiological pH
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?
[histone H3]-N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2
[histone H3]-L-lysine4 + succinate + formaldehyde + CO2
show the reaction diagram
[histone H3]-N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2
[histone H3]-L-lysine4 + succinate + formaldehyde + CO2
show the reaction diagram
[histone H3]-N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2
[histone H3]-N6,N6-L-lysine4 + succinate + formaldehyde + CO2
show the reaction diagram
[histone H3]-N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2
[histone H3]-L-lysine9 + succinate + formaldehyde + CO2
show the reaction diagram
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predicted site-specificity from phylogenetic analysis
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?
[histone H3]-N6,N6-methyl-L-lysine4 + 2-oxoglutarate + O2
[histone H3]-L-lysine4 + succinate + formaldehyde + CO2
show the reaction diagram
[histone H3]-N6,N6-methyl-L-lysine4 + 2-oxoglutarate + O2
[histone H3]-N6,N6-L-lysine4 + succinate + formaldehyde + CO2
show the reaction diagram
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?
[histone H3]-N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2
[histone H3]-N6,N6-L-lysine4 + succinate + formaldehyde + CO2
show the reaction diagram
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?
[histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2
[histone H3]-L-lysine4 + succinate + formaldehyde + CO2
show the reaction diagram
[p53]-methyl-L-lysine + 2-oxoglutarate + O2
[p53]-L-lysine + succinate + formaldehyde + CO2
show the reaction diagram
[protein p53]-N6,N6-dimethyl-L-lysine370 + 2-oxoglutarate + O2
[protein p53]-L-lysine370 + succinate + formaldehyde + CO2
show the reaction diagram
[protein p53]-N6-methyl-L-lysine370 + 2-oxoglutarate + O2
[protein p53]-L-lysine370 + succinate + formaldehyde + CO2
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dimethyl-histone 3 lysine 9 + ?
methyl-histone 3 lysine 9 + ?
show the reaction diagram
Q6PCM1
Jmjd1a demethylates dimethyl-histone 3 lysine 9 at the promoter regions of Tcl1, Tcfcp2l1, and Zfp57 and positively regulates the expression of these pluripotency-associated genes
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?
dimethylated histone 3 lysine 9 + ?
methyl-histone 3 lysine 9 + ?
show the reaction diagram
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proteins Swm1 and Swm2 associate together in a complex that specifically demethylates lysine 9 in histone H3 and both up- and down-regulates expression of different groups of genes. Protein Swm1 may act in concert with the histone deacetylase Clr6 and chromatin remodeller Hrp1 to repress gene expression
-
-
?
histone H3 N6,N6,N6-trimethyl-L-lysine9 + 2-oxoglutarate + O2
histone H3 N6,N6-dimethyl-L-lysine9 + succinate + formaldehyde + CO2
show the reaction diagram
histone H3 N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2
histone H3 N6-methyl-L-lysine9 + succinate + formaldehyde + CO2
show the reaction diagram
histone H3 N6-methyl-L-lysine9 + 2-oxoglutarate + O2
histone H3 L-lysine9 + succinate + formaldehyde + CO2
show the reaction diagram
histone H4 N6-methyl-L-lysine20 + 2-oxoglutarate + O2
histone H4 L-lysine20 + succinate + formaldehyde + CO2
show the reaction diagram
protein N6,N6-dimethyl-L-lysine + 2 2-oxoglutarate + 2 O2
protein L-lysine + 2 succinate + 2 formaldehyde + 2 CO2
show the reaction diagram
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?
[Dnmt1]-methyl-L-lysine + 2-oxoglutarate + O2
[Dnmt1]-L-lysine + succinate + formaldehyde + CO2
show the reaction diagram
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LSD1 demethylates the protein and regulates its function
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?
[Dnmt1]-methyl-L-lysine1096 + 2-oxoglutarate + O2
[Dnmt1]-L-lysine1096 + succinate + formaldehyde + CO2
show the reaction diagram
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mechanistic link between the histone and DNA methylation systems
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?
[histone H3]-N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2
[histone H3]-L-lysine4 + succinate + formaldehyde + CO2
show the reaction diagram
[histone H3]-N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2
[histone H3]-L-lysine4 + succinate + formaldehyde + CO2
show the reaction diagram
[histone H3]-N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2
[histone H3]-N6,N6-L-lysine4 + succinate + formaldehyde + CO2
show the reaction diagram
[histone H3]-N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2
[histone H3]-L-lysine9 + succinate + formaldehyde + CO2
show the reaction diagram
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predicted site-specificity from phylogenetic analysis
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?
[histone H3]-N6,N6-methyl-L-lysine4 + 2-oxoglutarate + O2
[histone H3]-L-lysine4 + succinate + formaldehyde + CO2
show the reaction diagram
[histone H3]-N6,N6-methyl-L-lysine4 + 2-oxoglutarate + O2
[histone H3]-N6,N6-L-lysine4 + succinate + formaldehyde + CO2
show the reaction diagram
-
-
-
-
?
[histone H3]-N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2
[histone H3]-N6,N6-L-lysine4 + succinate + formaldehyde + CO2
show the reaction diagram
-
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?
[histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2
[histone H3]-L-lysine4 + succinate + formaldehyde + CO2
show the reaction diagram
[p53]-methyl-L-lysine + 2-oxoglutarate + O2
[p53]-L-lysine + succinate + formaldehyde + CO2
show the reaction diagram
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LSD1 demethylates the protein and regulates its function
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?
[protein p53]-N6,N6-dimethyl-L-lysine370 + 2-oxoglutarate + O2
[protein p53]-L-lysine370 + succinate + formaldehyde + CO2
show the reaction diagram
O60341
LSD1 demethylates mono- and dimethylated Lys370 in the regulatory domain of the tumor suppressor p53, precluding the binding of the transcriptional coactivator 53BP1
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?
[protein p53]-N6-methyl-L-lysine370 + 2-oxoglutarate + O2
[protein p53]-L-lysine370 + succinate + formaldehyde + CO2
show the reaction diagram
O60341
LSD1 demethylates mono- and dimethylated Lys370 in the regulatory domain of the tumor suppressor p53, precluding the binding of the transcriptional coactivator 53BP1
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?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
flavin