Any feedback?
No. of entries
Information on EC 1.14.11.54 - mRNA N1-methyladenine demethylase
for references in articles please use BRENDA:EC1.14.11.54
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree 1 Oxidoreductases
1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
1.14.11.54 mRNA N1-methyladenine demethylase
IUBMB Comments
Contains iron(II). Catalyses oxidative demethylation of mRNA N1-methyladenine. The enzyme is also involved in alkylation repair in DNA .
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
- 1.14.11.54
-
paraneoplastic
-
pokeweed
-
anti-yo
-
anna-1
-
cd45ra
-
anti-il-6
-
cell-restricted
- medicine
Reaction Schemes
showN1-methyladenine in mRNA
+
+
=
adenine in mRNA
+
+
+
Synonyms
pca-1, alkb homolog 3, prostate cancer antigen-1, more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
ALKBH3
alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
ALKBH3
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
N1-methyladenine in mRNA + 2-oxoglutarate + O2 = adenine in mRNA + formaldehyde + succinate + CO2
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
mRNA-N1-methyladenine,2-oxoglutarate:oxygen oxidoreductase (formaldehyde-forming)
Contains iron(II). Catalyses oxidative demethylation of mRNA N1-methyladenine. The enzyme is also involved in alkylation repair in DNA [2].
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
5'-dAAAA-1-methyl-AYYAAA + 2-oxoglutarate + O2
5'-dAAAAAYYAAA + formaldehyde + succinate + CO2
Substrates: -
Products: -
Products: -
?
N1-methyladenine in mRNA + 2-oxoglutarate + O2
adenine in mRNA + formaldehyde + succinate + CO2
N1-methyladenine in ssDNA + 2-oxoglutarate + O2
adenine in ssDNA + formaldehyde + succinate + CO2
Substrates: -
Products: -
Products: -
?
N1-methyladenine in tRNA + 2-oxoglutarate + O2
adenine in tRNA + formaldehyde + succinate + CO2
Substrates: -
Products: -
Products: -
?
N3-ethylthymidine in mRNA + 2-oxoglutarate + O2
thymidine in mRNA + ?
Substrates: -
Products: -
Products: -
?
N3-methylcytidine in mRNA + 2-oxoglutarate + O2
cytidine in mRNA + formaldehyde + succinate + CO2
-
Substrates: -
Products: -
Products: -
?
N3-methylcytidine in tRNA + 2-oxoglutarate + O2
cytidine in tRNA + formaldehyde + succinate + CO2
Substrates: -
Products: -
Products: -
?
N6-methyladenine in tRNA + 2-oxoglutarate + O2
adenine in tRNA + formaldehyde + succinate + CO2
-
Substrates: -
Products: -
Products: -
?
RNA-N1-methyladenine + 2-oxoglutarate + O2
RNA-adenine + formaldehyde + succinate + CO2
Substrates: -
Products: -
Products: -
?
RNA-N6-methyladenine + 2-oxoglutarate + O2
RNA-adenine + formaldehyde + succinate + CO2
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: ALKBH3 demethylates 3-meC, 1-meA, and N6-meA in the presence of 2-oxoglutarate and Fe(II). ALKBH3 preferentially demethylates N6-meA in tRNA
Products: -
Products: -
-
N1-methyladenine in mRNA + 2-oxoglutarate + O2
adenine in mRNA + formaldehyde + succinate + CO2
-
Substrates: -
Products: -
Products: -
?
N1-methyladenine in mRNA + 2-oxoglutarate + O2
adenine in mRNA + formaldehyde + succinate + CO2
Substrates: -
Products: -
Products: -
?
N1-methyladenine in mRNA + 2-oxoglutarate + O2
adenine in mRNA + formaldehyde + succinate + CO2
-
Substrates: N1-methyladenine is prevalent in Homo sapiens mRNA, which shows an N1-methyladenine/adenine ratio of about 0.02%. Methylation of adenine in mRNA is reversible by ALKBH3. Recombinant ALKBH3 protein has robust demethylation activity against a synthetic RNA substrate containing a sitespecifically incorporated m1A modification
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
N1-methyladenine in mRNA + 2-oxoglutarate + O2
adenine in mRNA + formaldehyde + succinate + CO2
Substrates: -
Products: -
Products: -
?
N1-methyladenine in ssDNA + 2-oxoglutarate + O2
adenine in ssDNA + formaldehyde + succinate + CO2
Substrates: -
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Burkitt Lymphoma
Tp44 molecules involved in antigen-independent T cell activation are expressed on human plasma cells.
Carcinoma
Prostate cancer antigen-1 contributes to cell survival and invasion though discoidin receptor 1 in human prostate cancer.
Carcinoma
[Expression of prostate cancer antigen-1 in prostate cancer and its clinical significance]
Carcinoma, Hepatocellular
Association of AlkB homolog 3 expression with tumor recurrence and unfavorable prognosis in hepatocellular carcinoma.
Cerebellar Ataxia
Cerebrospinal fluid ?? T cell frequency is age-related: a case-control study of 435 children with inflammatory and non-inflammatory neurological disorders.
Digestive System Neoplasms
Emerging Roles of N6-Methyladenosine Demethylases and Its Interaction with Environmental Toxicants in Digestive System Cancers.
Down Syndrome
Purkinje cell cytoplasmic antibody type 1 (anti-yo) autoimmunity in a child with down syndrome.
Encephalitis
Cerebrospinal fluid ?? T cell frequency is age-related: a case-control study of 435 children with inflammatory and non-inflammatory neurological disorders.
Encephalomyelitis
Cerebrospinal fluid ?? T cell frequency is age-related: a case-control study of 435 children with inflammatory and non-inflammatory neurological disorders.
Encephalomyelitis, Acute Disseminated
Cerebrospinal fluid ?? T cell frequency is age-related: a case-control study of 435 children with inflammatory and non-inflammatory neurological disorders.
Lung Neoplasms
TP53 gene status is a critical determinant of phenotypes induced by ALKBH3 knockdown in non-small cell lung cancers.
Monoclonal Gammopathy of Undetermined Significance
Restricted expression of immunoglobulin light chain mRNA and of the adhesion molecule CD11b on circulating monoclonal B lineage cells in peripheral blood of myeloma patients.
Monoclonal Gammopathy of Undetermined Significance
Selective expression of CD45 isoforms defines CALLA+ monoclonal B-lineage cells in peripheral blood from myeloma patients as late stage B cells.
Multiple Myeloma
Elimination of myeloma cells from bone marrow by using monoclonal antibodies and magnetic immunobeads.
Multiple Myeloma
Restricted expression of immunoglobulin light chain mRNA and of the adhesion molecule CD11b on circulating monoclonal B lineage cells in peripheral blood of myeloma patients.
Multiple Myeloma
Selective expression of CD45 isoforms defines CALLA+ monoclonal B-lineage cells in peripheral blood from myeloma patients as late stage B cells.
Neoplasms
Anti-yo associated paraneoplastic cerebellar degeneration in a man with large cell cancer of the lung.
Neoplasms
Association of AlkB homolog 3 expression with tumor recurrence and unfavorable prognosis in hepatocellular carcinoma.
Neoplasms
Design and synthesis of prostate cancer antigen-1 (PCA-1/ALKBH3) inhibitors as anti-prostate cancer drugs.
Neoplasms
Elimination of myeloma cells from bone marrow by using monoclonal antibodies and magnetic immunobeads.
Neoplasms
Emerging Roles of N6-Methyladenosine Demethylases and Its Interaction with Environmental Toxicants in Digestive System Cancers.
Neoplasms
Paraneoplastic Cerebellar Degeneration (PCD) associated with PCA-1 antibodies in established cancer patients.
Neoplasms
Paraneoplastic gastrointestinal motor dysfunction: clinical and laboratory characteristics.
Neoplasms
Prostate cancer antigen-1 contributes to cell survival and invasion though discoidin receptor 1 in human prostate cancer.
Neoplasms
Proteins of human urine. II. Identification by two-dimensional electrophoresis of a new candidate marker for prostatic cancer.
Neoplasms
[Expression of prostate cancer antigen-1 in prostate cancer and its clinical significance]
Neoplasms, Second Primary
Paraneoplastic Cerebellar Degeneration (PCD) associated with PCA-1 antibodies in established cancer patients.
Nervous System Diseases
Purkinje cell cytoplasmic antibody type 1 (anti-yo) autoimmunity in a child with down syndrome.
Ovarian Neoplasms
Paraneoplastic Cerebellar Degeneration (PCD) associated with PCA-1 antibodies in established cancer patients.
Paraneoplastic Cerebellar Degeneration
Paraneoplastic Cerebellar Degeneration (PCD) associated with PCA-1 antibodies in established cancer patients.
Paraneoplastic Syndromes
Paraneoplastic Cerebellar Degeneration (PCD) associated with PCA-1 antibodies in established cancer patients.
Paraproteinemias
Restricted expression of immunoglobulin light chain mRNA and of the adhesion molecule CD11b on circulating monoclonal B lineage cells in peripheral blood of myeloma patients.
Paraproteinemias
Selective expression of CD45 isoforms defines CALLA+ monoclonal B-lineage cells in peripheral blood from myeloma patients as late stage B cells.
Plasmacytoma
Studies of in vitro activation and differentiation of human B lymphocytes. I. Phenotypic and functional characterization of the B cell population responding to anti-Ig antibody.
Pleural Effusion
Establishment of a monoclonal antibody to plasma cells: a comparison with CD38 and PCA-1.
Prostatic Hyperplasia
Anti-Tumor Effect of AlkB Homolog 3 Knockdown in Hormone- Independent Prostate Cancer Cells.
Prostatic Hyperplasia
Proteins of human urine. II. Identification by two-dimensional electrophoresis of a new candidate marker for prostatic cancer.
Prostatic Intraepithelial Neoplasia
High expression of a new marker PCA-1 in human prostate carcinoma.
Prostatic Intraepithelial Neoplasia
Prostate cancer antigen-1 as a potential novel marker for prostate cancer.
Prostatic Intraepithelial Neoplasia
[Expression of prostate cancer antigen-1 in prostate cancer and its clinical significance]
Prostatic Neoplasms
Anti-Tumor Effect of AlkB Homolog 3 Knockdown in Hormone- Independent Prostate Cancer Cells.
Prostatic Neoplasms
Clinical significance and therapeutic potential of prostate cancer antigen-1/ALKBH3 in human renal cell carcinoma.
Prostatic Neoplasms
Design and synthesis of prostate cancer antigen-1 (PCA-1/ALKBH3) inhibitors as anti-prostate cancer drugs.
Prostatic Neoplasms
Improving the bioavailability and anticancer effect of the PCA-1/ALKBH3 inhibitor HUHS015 using sodium salt.
Prostatic Neoplasms
Novel Metabolically Stable PCA-1/ALKBH3 Inhibitor Has Potent Antiproliferative Effects on DU145 Cells
Prostatic Neoplasms
Prostate cancer antigen-1 as a potential novel marker for prostate cancer.
Prostatic Neoplasms
Prostate cancer antigen-1 contributes to cell survival and invasion though discoidin receptor 1 in human prostate cancer.
Prostatic Neoplasms
[Effect of downregulation of prostate cancer antigen-1 expression on malignant biological behavior of prostate cancer LNCaP cells].
Prostatic Neoplasms
[Expression of prostate cancer antigen-1 in prostate cancer and its clinical significance]
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00232
RNA-N1-methyladenine
wild type enzyme, pH and temperature not specified in the publication
-
0.00276
RNA-N6-methyladenine
mutant enzyme D135I, pH and temperature not specified in the publication
-
0.00347
RNA-N6-methyladenine
mutant enzyme E136L, pH and temperature not specified in the publication
-
0.00577
RNA-N6-methyladenine
mutant enzyme D135N, pH and temperature not specified in the publication
-
0.01493
RNA-N6-methyladenine
wild type enzyme, pH and temperature not specified in the publication
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.062
RNA-N1-methyladenine
wild type enzyme, pH and temperature not specified in the publication
-
0.0016
RNA-N6-methyladenine
mutant enzyme D135I, pH and temperature not specified in the publication
-
0.0017
RNA-N6-methyladenine
mutant enzyme D135N, pH and temperature not specified in the publication
-
0.0017
RNA-N6-methyladenine
mutant enzyme E136L, pH and temperature not specified in the publication
-
0.0018
RNA-N6-methyladenine
wild type enzyme, pH and temperature not specified in the publication
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
26.7
RNA-N1-methyladenine
wild type enzyme, pH and temperature not specified in the publication
-
0.12
RNA-N6-methyladenine
wild type enzyme, pH and temperature not specified in the publication
-
0.3
RNA-N6-methyladenine
mutant enzyme D135N, pH and temperature not specified in the publication
-
0.5
RNA-N6-methyladenine
mutant enzyme E136L, pH and temperature not specified in the publication
-
0.58
RNA-N6-methyladenine
mutant enzyme D135I, pH and temperature not specified in the publication
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
1-methyladenine and 3-methylcytosine lesions in DNA/RNA contribute to the cytotoxicity of methylating agents. These lesions are directly reversed by ABH3 in humans
physiological function
ALKBH3 binds to transcription associated locations, such as places of promoter-proximal paused RNA polymerase II and enhancers. ALKBH3 strongly binds to the transcription initiation sites of a small number of highly active gene promoters characterized by high levels of transcriptional regulators, including transcription factors, the Mediator complex, cohesin, histone modifiers, and active histone marks. ALKBH3 does not directly influence the transcription of its target genes, but its depletion induces an upregulation of ALKBH3 non-bound inflammatory genes
physiological function
ALKBH3 is associated with the activating signal cointegrator complex ASCC. ALKBH3 is overexpressed in various cancers, and both ALKBH3 and ASCC are important for alkylation damage resistance in these tumor cell lines. ASCC3, the largest subunit of ASCC, encodes a 3'-5' DNA helicase, whose activity is crucial for the generation of single-stranded DNA upon which ALKBH3 preferentially functions for dealkylation. In cell lines that are dependent on ALKBH3 and ASCC3 for alkylation damage resistance, loss of ALKBH3 or ASCC3 leads to increased 3-methylcytosine and reduced cell proliferation. ALKBH3 catalytic activity is important for alkylation damage resistance
physiological function
human adenocarcinomas and squamous cell carcinomas of the lung show overexpression of Alkbh3 and the percentage of cells positive for Alkbh3 also correlates statistically to recurrence-free survival in adenocarcinoma. Knockdown of Alkbh3 by siRNA transfection induces expression of p21WAF1/Cip1 and p27Kip1 in the human lung adenocarcinoma cell line A-549, resulting in cell cycle arrest, senescence and strong suppression of cell growth in vitro. In vivo, peritoneal tumour growth and dissemination is inhibited in nude mice, previously inoculated with the A-549 cell line, by intraperitoneal injection of Alkbh3 siRNA plus atelocollagen
physiological function
-
N1-methyladenine in mRNA can be reversed by ALKBH3. Adenine methylation responds dynamically to stimuli, and hundreds of stress-induced N1-methyladenine sites can be identified. Knockout and overexpression of ALKBH3 in HEK-293T cells leads to an increased or decreased N1-methyladenine/adenine ratio in mRNA, respectively
physiological function
the absence of Alkbh3 does not lead to a statistically significant change in the transcriptional blockage or mutagenic properties of O2-ethyl-deoxythymidine, O4-ethyl-deoxythymidine, N3-carboxymethyl-deoxythymidine, and O4-carboxymethyl-deoxythymidine in mammalian cells. N3-ethyl-deoxythymidine induces a substantially higher degree of A to U mutation (about 31%) in Alkbh3-deficient cells than in the wildtype background. N3-ethyl-deoxythymidine, but not other lesions, can be repaired by Alkbh3 in mammalian cells
physiological function
-
ALKBH3 effectively demethylates 1-meA and 3-meC within endogenously methylated RNA and also N6-methyladenine (N6-meA) in mammalian transfer RNA. ALKBH3-demethylated tRNA significantly enhances protein translation efficiency. ALKBH3 knockdown in human cancer cells impairs cellular proliferation and suppresses the nascent protein synthesis that is usually accompanied by accumulation of the methylated RNAs
physiological function
ALKBH3 is a 1-methyladenosine and 3-methylcytidine demethylase of tRNA. ALKBH3 can promote cancer cell proliferation, migration and invasion. The m1A demethylated tRNA is more sensitive to angiogenin cleavage, followed by generating tRNA-derived small RNAs around the anticodon regions. in ALKBH3-/- HeLa cells, m1A level of tRNA in cytoplasm, but not in nucleus, is remarkably higher than that of wild type cells. Deletion of ALKBH3 significantly increases the m1A levels of most target tRNAs such as tRNAGluCTC, tRNASeCTCA, tRNAGlyGCC, tRNAHisGTG, tRNASerGCT, tRNAGlnCTG, tRNAValCAC, tRNAArgCCT, tRNALeuCAA, tRNAGlyCCC, tRNAAlaCGC and tRNAeMetCAT. m1A levels of some targets, such as tRNALysCTT and tRNAAsnGTT, are not affected. m3C levels of target tRNA are significantly upregulated in Alkbh3-/- HeLa cells
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). ALKB3_BOVIN
286
0
33156
Swiss-Prot
Mitochondrion (Reliability: 5)
ALKB3_HUMAN
286
0
33375
Swiss-Prot
Mitochondrion (Reliability: 3)
ALKB3_MOUSE
286
0
33061
Swiss-Prot
other Location (Reliability: 5)
ALKB3_RAT
295
0
34011
Swiss-Prot
Mitochondrion (Reliability: 4)
A0A6J8D7R1_MYTCO
277
0
32098
TrEMBL
other Location (Reliability: 5)
E3TFN3_ICTPU
276
0
31478
TrEMBL
other Location (Reliability: 5)
A0A8M1N1B3_DANRE
282
0
32046
TrEMBL
Mitochondrion (Reliability: 5)
A0A6J8B5X9_MYTCO
192
0
22353
TrEMBL
other Location (Reliability: 5)
A0A1Z5JI97_FISSO
713
0
81072
TrEMBL
other Location (Reliability: 1)
A0A812CN77_SEPPH
258
0
30118
TrEMBL
other Location (Reliability: 2)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
structure of the ABH3 catalytic core in complex with iron and 2-oxoglutarate at 1.5 A resolution. The ABH3 structure reveals the beta-strand jelly-roll fold that coordinates a catalytically active iron centre by a conserved His1-X-Asp/Glu-Xn-His2 motif. The structure shows a positively charged DNA/RNA binding groove and a flexible hairpin involved in nucleotide flipping and ss/ds-DNA discrimination, and reveals self-hydroxylation of an active site leucine
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D135I
the mutant shows increased activity with RNA-N6-methyladenine and reduced activity with DNA-N1-methyladenine compared to the wild type enzyme
D135N
the mutant shows increased activity with RNA-N6-methyladenine and reduced activity with DNA-N1-methyladenine compared to the wild type enzyme
E136L
the mutant shows increased activity with RNA-N6-methyladenine and reduced activity with DNA-N1-methyladenine compared to the wild type enzyme
L177A
mutation at the active site, mutant lost its demethylation activity on m1A of tRNA in vitro and in vivo
R122A
mutant is defective in its DNA/RNA binding groove, mutant lost its demethylation activity on m1A of tRNA in vitro and in vivo
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HisTrap affinity column chromatography, and Mono Q 5/50 GL anion ion exchange column chromatography
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
medicine
human adenocarcinomas and squamous cell carcinomas of the lung show overexpression of Alkbh3 and the percentage of cells positive for Alkbh3 also correlates statistically to recurrence-free survival in adenocarcinoma. Knockdown of Alkbh3 by siRNA transfection induces expression of p21WAF1/Cip1 and p27Kip1 in the human lung adenocarcinoma cell line A-549, resulting in cell cycle arrest, senescence and strong suppression of cell growth in vitro. In vivo, peritoneal tumour growth and dissemination is inhibited in nude mice, previously inoculated with the A-549 cell line, by intraperitoneal injection of Alkbh3 siRNA plus atelocollagen
medicine
ALKBH3 promoter CpG island hypermethylation is most frequent in Hodgkin lymphoma cell lines (44%; 4 of 9), followed by Burkitt lymphoma (38%; 5 of 13), other non-Hodgkin lymphoma cell lines (37%; 12 of 32), and anaplastic large cell lymphoma. ALKBH3 promoter hypermethylation is detected in detected in18% (14 of 80) of the primary Hodgkin lymphoma cases. ALKBH3 CpG island hypermethylation is associated with protein loss. ALKBH3 epigenetic silencing is also associated with COL1A2 and COL1A1 overexpression, whereas an unmethylated ALKBH3 CpG island is linked to the absence of collagen expression. ALKBH3 hypermethylation is associated with shorter overall survival in the studied Hodgkin lymphoma cohort
medicine
the alteration of ALKBH3 expression regulates the cytokine CSF-1 mRNA stability. Actions of cytokine CSF-1 lead to poor prognosis in ovarian and breast cancers. Demethylation of m1A by ALKBH3 increases the half-life of CSF-1 mRNA without affecting the translation efficiency. Overexpression of ALKBH3 increases CSF-1 expression and the degree of cancer cell invasiveness without affecting cell proliferation or migration
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
You, C.; Wang, P.; Nay, S.L.; Wang, J.; Dai, X.; OConnor, T.R.; Wang, Y.
Roles of Aag, Alkbh2, and Alkbh3 in the repair of carboxymethylated and ethylated thymidine lesions
ACS Chem. Biol.
11
1332-1338
2016
Mus musculus (Q8K1E6)
brenda
Tasaki, M.; Shimada, K.; Kimura, H.; Tsujikawa, K.; Konishi, N.
ALKBH3, a human AlkB homologue, contributes to cell survival in human non-small-cell lung cancer
Br. J. Cancer
104
700-706
2011
Homo sapiens (Q96Q83)
brenda
Sundheim, O.; Vagbo, C.; Bjoras, M.; Sousa, M.; Talstad, V.; Aas, P.; Drablos, F.; Krokan, H.; Tainer, J.; Slupphaug, G.
Human ABH3 structure and key residues for oxidative demethylation to reverse DNA/RNA damage
EMBO J.
25
3389-3397
2006
Homo sapiens (Q96Q83)
brenda
Liefke, R.; Windhof-Jaidhauser, I.M.; Gaedcke, J.; Salinas-Riester, G.; Wu, F.; Ghadimi, M.; Dango, S.
The oxidative demethylase ALKBH3 marks hyperactive gene promoters in human cancer cells
Genome Med.
7
66
2015
Homo sapiens (Q96Q83)
brenda
Dango, S.; Mosammaparast, N.; Sowa, M.E.; Xiong, L.J.; Wu, F.; Park, K.; Rubin, M.; Gygi, S.; Harper, J.W.; Shi, Y.
DNA unwinding by ASCC3 helicase is coupled to ALKBH3-dependent DNA alkylation repair and cancer cell proliferation
Mol. Cell
44
373-384
2011
Homo sapiens (Q96Q83)
brenda
Li, X.; Xiong, X.; Wang, K.; Wang, L.; Shu, X.; Ma, S.; Yi, C.
Transcriptome-wide mapping reveals reversible and dynamic N(1)-methyladenosine methylome
Nat. Chem. Biol.
12
311-316
2016
Homo sapiens
brenda
Zhu, C.; Yi, C.
Switching demethylation activities between AlkB family RNA/DNA demethylases through exchange of active-site residues
Angew. Chem. Int. Ed. Engl.
53
3659-3662
2014
Escherichia coli (P05050)
brenda
Ueda, Y.; Kitae, K.; Ooshio, I.; Fusamae, Y.; Kawaguchi, M.; Jingushi, K.; Harada, K.; Hirata, K.; Tsujikawa, K.
A real-time PCR-based quantitative assay for 3-methylcytosine demethylase activity of ALKBH3
Biochem. Biophys. Rep.
5
476-481
2016
Homo sapiens (Q96Q83), Homo sapiens
brenda
Beharry, A.A.; Lacoste, S.; OConnor, T.R.; Kool, E.T.
Fluorescence monitoring of the oxidative repair of DNA alkylation damage by ALKBH3, a prostate cancer marker
J. Am. Chem. Soc.
138
3647-3650
2016
Homo sapiens (Q96Q83)
brenda
Woo, H.H.; Chambers, S.K.
Human ALKBH3-induced m1A demethylation increases the CSF-1 mRNA stability in breast and ovarian cancer cells
Biochim. Biophys. Acta Gene Regul. Mech.
1862
35-46
2019
Homo sapiens (Q96Q83)
brenda
Esteve-Puig, R.; Climent, F.; Pineyro, D.; Domingo-Domenech, E.; Davalos, V.; Encuentra, M.; Rea, A.; Espejo-Herrera, N.; Soler, M.; Lopez, M.; Ortiz-Barahona, V.; Tapia, G.; Navarro, J.; Cid, J.; Farre, L.; Villanueva, A.; Casanova, I.; Mangues, R.
Epigenetic loss of m1A RNA demethylase ALKBH3 in Hodgkin lymphoma targets collagen, conferring poor clinical outcome
Blood
137
994-999
2021
Homo sapiens (Q96Q83)
brenda
Chen, Z.; Qi, M.; Shen, B.; Luo, G.; Wu, Y.; Li, J.; Lu, Z.; Zheng, Z.; Dai, Q.; Wang, H.
Transfer RNA demethylase ALKBH3 promotes cancer progression via induction of tRNA-derived small RNAs
Nucleic Acids Res.
47
2533-2545
2019
Homo sapiens (Q96Q83)
brenda
Ueda, Y.; Ooshio, I.; Fusamae, Y.; Kitae, K.; Kawaguchi, M.; Jingushi, K.; Hase, H.; Harada, K.; Hirata, K.; Tsujikawa, K.
AlkB homolog 3-mediated tRNA demethylation promotes protein synthesis in cancer cells
Sci. Rep.
7
42271
2017
Bombyx mori
brenda
EXTERNAL LINKS (specific for EC-Number 1.14.11.54)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
About BRENDA
Social media
Help
Survey
Download
Contribution
Legal
Curated at
Member of
