BRENDA home
History
Information on EC 1.14.11.44 - (R)-dichlorprop dioxygenase (2-oxoglutarate)
for references in articles please use BRENDA:EC1.14.11.44Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
1.14.11.44 (R)-dichlorprop dioxygenase (2-oxoglutarate)IUBMB Comments
Fe2+-dependent enzyme. The enzymes from the Gram-negative bacteria Delftia acidovorans MC1 and Sphingomonas herbicidovorans MH are involved in the degradation of the (R)-enantiomer of the phenoxyalkanoic acid herbicides mecoprop and dichlorprop [1,2].
Specify your search results
Word Map
- 1.14.11.44
-
herbicide
-
aryloxyalkanoate
- maize
-
2,4-dichlorophenoxyacetic
-
aryloxyphenoxypropionate
- corn
- grain
-
glyphosate-resistant
- sugarcane
-
sphingobium
-
agronomically
-
enantioselective
-
quizalofop-p-ethyl
-
herbicide-tolerant
- accase
- auxin
-
non-transgenic
-
bacilliform
-
toxicological
- carboxylase
- glyphosate
- ubiquitin
-
non-gm
-
weed
-
near-isogenic
The enzyme appears in viruses and cellular organisms
Reaction Schemes
+
+
=
+
+
+
+
+
=
+
+
+
Synonyms
(R)-phenoxypropionate/alpha-ketoglutarate-dioxygenase, 2-oxoglutarate-dependent (R)-dichlorprop dioxygenase, AAD-1, alpha-ketoglutarate-dependent (R)-dichlorprop dioxygenase, ariloxyalkanoate-dioxygenase-1, RdpA, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
(R)-phenoxypropionate/alpha-ketoglutarate-dioxygenase
AAD-1
alpha-ketoglutarate-dependent (R)-dichlorprop dioxygenase
-
ariloxyalkanoate-dioxygenase-1
RdpA
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2 = 2,4-dichlorophenol + pyruvate + succinate + CO2
(2)
-
-
-
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2 = 4-chloro-2-methylphenol + pyruvate + succinate + CO2
(1)
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
((R)-2-(4-chloro-2-methylphenoxy)propanoate,2-oxoglutarate:oxygen oxidoreductase (pyruvate-forming)
Fe2+-dependent enzyme. The enzymes from the Gram-negative bacteria Delftia acidovorans MC1 and Sphingomonas herbicidovorans MH are involved in the degradation of the (R)-enantiomer of the phenoxyalkanoic acid herbicides mecoprop and dichlorprop [1,2].
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2
2,4-dichlorophenol + pyruvate + succinate + CO2
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2
4-chloro-2-methylphenol + pyruvate + succinate + CO2
additional information
?
-
construction of homology models of SdpA and RdpA from Sphingomonas herbicidovorans MH and the use of docking to identify residues likely to be involved in herbicide binding
-
-
?
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2
2,4-dichlorophenol + pyruvate + succinate + CO2
i.e. (R)-dichlorprop, the enzyme is involved in degrading the chiral herbicides (RS)-2-(2,4-dichlorophenoxy)propionate (dichlorprop) and (RS)-2-(4-chloro-2-methylphenoxy)propionate (mecoprop). RdpA is highly specific for the cleavage of the R-configuration of dichlorprop and mecoprop
-
-
?
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2
2,4-dichlorophenol + pyruvate + succinate + CO2
i.e. (R)-dichlorprop, the enzyme is involved in degrading the chiral herbicides (RS)-2-(2,4-dichlorophenoxy)propionate (dichlorprop) and (RS)-2-(4-chloro-2-methylphenoxy)propionate (mecoprop). RdpA is highly specific for the cleavage of the R-configuration of dichlorprop and mecoprop
-
-
?
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2
2,4-dichlorophenol + pyruvate + succinate + CO2
i.e. (R)-dichlorprop. RdpA exclusively transforms the (R)-enantiomer of dichlorprop
-
-
?
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2
2,4-dichlorophenol + pyruvate + succinate + CO2
i.e. (R)-dichlorprop. RdpA exclusively transforms the (R)-enantiomer of dichlorprop
-
-
?
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2
2,4-dichlorophenol + pyruvate + succinate + CO2
i.e. (R)-dichlorprop
-
-
?
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2
2,4-dichlorophenol + pyruvate + succinate + CO2
the enzyme catalyzes the initial step of microbial degradation of the chiral herbicide (R,S)-2-(2,4-dichlorophenoxy)propionate (R,S-dichlorprop)
-
-
?
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2
2,4-dichlorophenol + pyruvate + succinate + CO2
i.e. (R)-dichlorprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH
-
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2
4-chloro-2-methylphenol + pyruvate + succinate + CO2
i.e. (R)-mecoprop, the enzyme is involved in degrading the chiral herbicides (RS)-2-(2,4-dichlorophenoxy)propionate (dichlorprop) and (RS)-2-(4-chloro-2-methylphenoxy)propionate (mecoprop). RdpA is highly specific for the cleavage of the R-configuration of dichlorprop and mecoprop
-
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2
4-chloro-2-methylphenol + pyruvate + succinate + CO2
i.e. (R)-mecoprop, the enzyme is involved in degrading the chiral herbicides (RS)-2-(2,4-dichlorophenoxy)propionate (dichlorprop) and (RS)-2-(4-chloro-2-methylphenoxy)propionate (mecoprop). RdpA is highly specific for the cleavage of the R-configuration of dichlorprop and mecoprop
-
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2
4-chloro-2-methylphenol + pyruvate + succinate + CO2
-
-
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2
4-chloro-2-methylphenol + pyruvate + succinate + CO2
i.e. (R)-mecoprop. RdpA exclusively transforms the (R)-enantiomer of mecoprop. The enzyme is specific for 2-oxoglutarate. Low levels of activity (1.9%) in presence of 2-oxoadipate. No activity with oxaloacetate, 2-oxobutyrate, and 2-oxovalerate
-
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2
4-chloro-2-methylphenol + pyruvate + succinate + CO2
i.e. (R)-mecoprop. RdpA exclusively transforms the (R)-enantiomer of mecoprop
-
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2
4-chloro-2-methylphenol + pyruvate + succinate + CO2
i.e. (R)-mecoprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH
-
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2
4-chloro-2-methylphenol + pyruvate + succinate + CO2
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2
2,4-dichlorophenol + pyruvate + succinate + CO2
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2
4-chloro-2-methylphenol + pyruvate + succinate + CO2
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2
2,4-dichlorophenol + pyruvate + succinate + CO2
i.e. (R)-dichlorprop, the enzyme is involved in degrading the chiral herbicides (RS)-2-(2,4-dichlorophenoxy)propionate (dichlorprop) and (RS)-2-(4-chloro-2-methylphenoxy)propionate (mecoprop). RdpA is highly specific for the cleavage of the R-configuration of dichlorprop and mecoprop
-
-
?
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2
2,4-dichlorophenol + pyruvate + succinate + CO2
i.e. (R)-dichlorprop, the enzyme is involved in degrading the chiral herbicides (RS)-2-(2,4-dichlorophenoxy)propionate (dichlorprop) and (RS)-2-(4-chloro-2-methylphenoxy)propionate (mecoprop). RdpA is highly specific for the cleavage of the R-configuration of dichlorprop and mecoprop
-
-
?
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2
2,4-dichlorophenol + pyruvate + succinate + CO2
i.e. (R)-dichlorprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH
-
-
?
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2
2,4-dichlorophenol + pyruvate + succinate + CO2
the enzyme catalyzes the initial step of microbial degradation of the chiral herbicide (R,S)-2-(2,4-dichlorophenoxy)propionate (R,S-dichlorprop)
-
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2
4-chloro-2-methylphenol + pyruvate + succinate + CO2
i.e. (R)-mecoprop, the enzyme is involved in degrading the chiral herbicides (RS)-2-(2,4-dichlorophenoxy)propionate (dichlorprop) and (RS)-2-(4-chloro-2-methylphenoxy)propionate (mecoprop). RdpA is highly specific for the cleavage of the R-configuration of dichlorprop and mecoprop
-
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2
4-chloro-2-methylphenol + pyruvate + succinate + CO2
i.e. (R)-mecoprop, the enzyme is involved in degrading the chiral herbicides (RS)-2-(2,4-dichlorophenoxy)propionate (dichlorprop) and (RS)-2-(4-chloro-2-methylphenoxy)propionate (mecoprop). RdpA is highly specific for the cleavage of the R-configuration of dichlorprop and mecoprop
-
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2
4-chloro-2-methylphenol + pyruvate + succinate + CO2
-
-
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2
4-chloro-2-methylphenol + pyruvate + succinate + CO2
i.e. (R)-mecoprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH
-
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2
4-chloro-2-methylphenol + pyruvate + succinate + CO2
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe2+
ferrous iron is necessary for activity of the enzyme, and other divalent cations could not replace it
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(S)-2-(4-chloro-2-methylphenoxy)propanoate
Q93A variant retains 60% of wild-type enzyme activity, but this drops to 35% when the racemate is provided, suggesting that Gln93 prevents the incorrect enantiomer from binding to and inhibiting the enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ascorbate
slightly reduced activity in the absence of ascorbate
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.099
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C
0.108
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C, mutant enzyme F171A
0.38
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C, wild-type enzyme
0.438
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C, mutant enzyme F171Q
1.55
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C, mutant enzyme R285A
2.3
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C, mutant enzyme V80A
2.8
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C, mutant enzyme Q93A
8.9
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C, mutant enzyme I106A
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.87
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C, mutant enzyme R285A
2.9
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C, mutant enzyme F171A
3.38
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C, mutant enzyme F171Q
4
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C, mutant enzyme I106A
4.13
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C, mutant enzyme V80A
4.2
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C, wild-type enzyme
4.33
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C, mutant enzyme Q93A
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.45
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C, mutant enzyme I106A
0.56
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C, mutant enzyme R285A
1.55
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C, mutant enzyme Q93A
1.8
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C, mutant enzyme V80A
7.7
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C, mutant enzyme F171Q
11
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C, wild-type enzyme
26.9
(R)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30°C, mutant enzyme F171A
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 7.5
pH 6.0: about 60% of maximal activity, pH 7.5: about 55% of maximal activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). RDPA_SPHHM
Sphingobium herbicidovorans (strain ATCC 700291 / DSM 11019 / NBRC 16415 / MH)
295
0
33213
Swiss-Prot
-
A0A2S6SHI1_9PROT
276
0
32034
TrEMBL
-
A0A2S6TE18_9PROT
282
0
31987
TrEMBL
-
A0A2S6SEY6_9PROT
280
0
32443
TrEMBL
-
A0A2S6TIR0_9PROT
288
0
31782
TrEMBL
-
A0A2S6UHL6_9PROT
278
0
31083
TrEMBL
-
A0A6J5GY81_9BURK
264
0
29692
TrEMBL
-
A0A2P5VLK9_9PROT
279
0
31522
TrEMBL
-
A0A2S6Q683_9PROT
291
0
32786
TrEMBL
-
A0A2P2E6X3_9PROT
273
0
30431
TrEMBL
-
A0A2S6TJZ1_9PROT
289
0
32834
TrEMBL
-
A0A2S6UR65_9PROT
305
0
34936
TrEMBL
-
A0A2S6TR69_9PROT
282
0
32028
TrEMBL
-
A0A2S6U1Q5_9PROT
296
0
33092
TrEMBL
-
A0A2S6U2I1_9PROT
231
0
25283
TrEMBL
-
A0A2S6USD6_9PROT
259
0
30074
TrEMBL
-
A0A2S6TIY1_9PROT
280
0
31565
TrEMBL
-
A0A2S6TQ80_9PROT
295
0
33174
TrEMBL
-
A0A2S6S469_9PROT
281
0
32267
TrEMBL
-
A0A2S6U972_9PROT
246
0
27908
TrEMBL
-
A0A2S6QNK7_9PROT
278
0
32304
TrEMBL
-
A0A644XW05_9ZZZZ
291
0
32729
TrEMBL
other Location (Reliability: 2)
A0A2S6T2S4_9PROT
276
0
32020
TrEMBL
-
A0A2S6SJS8_9PROT
275
0
31807
TrEMBL
-
A0A2S6SVT4_9PROT
280
0
32443
TrEMBL
-
A0A2S6Q6L3_9PROT
281
0
32450
TrEMBL
-
A0A2S6S886_9PROT
280
0
32216
TrEMBL
-
A0A2S6TMY8_9PROT
299
0
34034
TrEMBL
-
A0A2S6QDE5_9PROT
279
0
31795
TrEMBL
-
A0A2S6T7G1_9PROT
255
0
29224
TrEMBL
-
A0A2S6U212_9PROT
283
0
31328
TrEMBL
-
A0A2R8BD12_9RHOB
272
0
30047
TrEMBL
-
A0A2S6QEF9_9PROT
282
0
31876
TrEMBL
-
A0A2S6QEF0_9PROT
301
0
33945
TrEMBL
-
A0A2S6QEL0_9PROT
294
0
33953
TrEMBL
-
A0A2S6U356_9PROT
290
0
32475
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PDB
SCOP
CATH
UNIPROT
ORGANISM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
36000
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
trimer
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
F171A
exhibits about 70% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 245% compared to the wild-type value
F171Q
exhibits about 70% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 70% compared to the wild-type value
I106A
exhibits about 30% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 5% compared to the wild-type value
I106G/G107I
exhibits 0.7% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate
I106G/G107N
exhibits 8.7% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate
L83A
exhibits about 10% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate
Q93A
exhibits about 60% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 14% compared to the wild-type value
R285A
V80A
exhibits about 60% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 17% compared to the wild-type value
Y221A
R285A
exhibits 14.8% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate. kcat/Km is 5% compared to the wild-type value
R285A
exhibits about 10% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate
Y221A
exhibits 5.2% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate
Y221A
exhibits about 10% of wild-type enzyme activity with (R)-2-(4-chloro-2-methylphenoxy)propanoate
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as His6-tagged fusion protein from Escherichia coli BL21(DE3)(pLysS)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
rdpA and sdpA gene expression is constitutive at a basal level but is induced in response to (R,S)-dichlorprop degradation under in situ conditions
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Müller, T.A.; Fleischmann, T.; van der Meer, J.R.; Kohler, H.P.
Purification and characterization of two enantioselective alpha-ketoglutarate-dependent dioxygenases, RdpA and SdpA, from Sphingomonas herbicidovorans MH
Appl. Environ. Microbiol.
72
4853-4861
2006
Sphingobium herbicidovorans (Q8KSC8), Sphingobium herbicidovorans
brenda
Paulin, M.M.; Nicolaisen, M.H.; Sorensen, J.
Abundance and expression of enantioselective rdpA and sdpA dioxygenase genes during degradation of the racemic herbicide (R,S)-2-(2,4-dichlorophenoxy)propionate in soil
Appl. Environ. Microbiol.
76
2873-2883
2010
Sphingobium herbicidovorans (Q8KSC8)
brenda
Westendorf, A.; Benndorf, D.; Müller, R.H.; Babel, W.
The two enantiospecific dichlorprop/alpha-ketoglutarate-dioxygenases from Delftia acidovorans MC1 - protein and sequence data of RdpA and SdpA
Microbiol. Res.
157
317-322
2002
Delftia acidovorans (P83310), Delftia acidovorans, Delftia acidovorans MC1 (P83310), Delftia acidovorans MC1
brenda
Müller, T.A.; Zavodszky, M.I.; Feig, M.; Kuhn, L.A.; Hausinger, R.P.
Structural basis for the enantiospecificities of R- and S-specific phenoxypropionate/alpha-ketoglutarate dioxygenases
Protein Sci.
15
1356-1368
2006
Sphingobium herbicidovorans (Q8KSC8)
brenda
Queiroz, A.; Vidal, R.
The development of dichlorophenoxyacetate herbicide tolerant crops Literature review
Planta Daninha
32
649-654
2014
Sphingobium herbicidovorans (Q8KSC8), Sphingobium herbicidovorans MH (Q8KSC8)
-
brenda
Select items on the left to see more content.
EXTERNAL LINKS (specific for EC-Number 1.14.11.44)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
About BRENDA
Help
Download
Contribution
member of
curated at
curated at
Server: brenda5 Ver: 9edf756-main (2022-05-17 10:30:03 +0200)