Information on EC 1.14.11.20 - deacetoxyvindoline 4-hydroxylase

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SOURCE TISSUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

SOURCE

cell suspension culture

brenda

brenda

no association between plantlet formation and other biosynthetic enzymes such as deacetoxyvindoline 4-hydroxylase and tryptophan decarboxylase, is found

brenda

brenda

etiolated seed, contains considerable levels of transcript but almost undetectable enzymic activity. Exposure of seedlings to light results in a rapid increase of enzyme activity without a significant further increase in d4h transcripts over those detected in dark-grown seedlings

brenda

brenda

brenda

cell suspension culture

brenda

cell suspension culture

from leaf

brenda

low expression

brenda

5% of activity in leaf

brenda

leaf

440241, 712866, 726538

brenda

brenda

brenda

highest activity in leaf

brenda

predominantly in young leaf

brenda

suspension-cultured cell from leaf

brenda

low expression

brenda

8% of activity in leaf

brenda

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LOCALIZATION

ORGANISM

UNIPROT

COMMENTARY

GeneOntology No.

LITERATURE

SOURCE

nucleoplasm

the enzyme resides in the nucleocytoplasmic compartment following passive diffusion to the nucleus allowed by the protein size. Colocalization with deacetylvindoline-4-O-acetyltransferase

5654

brenda

additional information

vindoline biosynthesis pathway enzyme subcellular localization study, overview

brenda

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GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

metabolism

3 entries

physiological function

part of vindoline biosynthesis

684439

metabolism

the enzyme catalyzes the penultimate step in the biosynthesis of vindoline, which constitutes the main terpenoid indole alkaloid accumulated in leaves of Catharanthus roseus, spatial organization of the vindoline biosynthetic pathway, overview. The desacetoxyvindoline-4-hydroxylase interacts directly with the deacetylvindoline-4-O-acetyltransferase, that catalyzes the last step of the biosynthetic pathway, absence of metabolic channeling in the vindoline biosynthetic pathway. Importance of inter- and intracellular translocations of intermediates during the vindoline biosynthesis and potential regulatory role, overview

metabolism

the enzyme is involved in biosynthesis of terpenoidindole alkaloids

metabolism

the enzyme is involved in terpenoid indole alkaloid (TIA) biosynthetic pathway

743812

Go to AA Sequence and Transmembrane Helices SearchGo to Localization Prediction

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

44700

1 * 44700, SDS-PAGE

45000

45647

* 45647, calculated

45000

45000

gel filtration

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SUBUNIT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

* 45647, calculated

monomer

monomer

monomer

1 * 44700, SDS-PAGE

Go to Purification (commentary) Search

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

440243

Sephadex G-100, Green 19-agarose, hydroxylapatite, 2-oxoglutarate-Sepharose, Mono-Q

440242

Go to Cloned (commentary) Search

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

440245

cloning of cDNA, expression in Escherichia coli, possibly dimorphic allele of a single-copy gene

cloning of cDNA, possibly dimorphic allele of a single-copy gene

gene D4H, semi-quantitative RT-PCR expression analysis

Go to Expression Search

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EXPRESSION

ORGANISM

UNIPROT

LITERATURE

artemisinic acid is able to upregulate the transcriptions of deacetoxyvindoline 4-hydroxylase

artemisinic acid leads to upregulation of the deacetoxyvindoline 4-hydroxylase and upstream enzymes of the vinblastine biosynthesis, i.e. tryptophan decarboxylase, geraniol 10-hydroxylase, tabersonine 16-hydroxylase

significant upregulation in salicylic acid treatment (foliar application of 0.01 and 0.1 mM salicylic acid). Upregulation can result in a higher production rate of vinblastine and vincristine alkaloids

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765375

upregulated in plants inoculated with endophytes

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APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

synthesis

2 entries

synthesis

maximal production of vindoline coincides with maximal activity of enzyme and of deacetylvindoline acetyl-CoA acetyl transferase and tryptophan decarboxylase

655109

synthesis

artemisinic acid can be used as a promising elicitor, especially for the production of therapeutically important indole alkaloids

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

440241

De Carolis, E.; Chan, F.; Balsevich, J.; De Luca, V.

Isolation and characterization of a 2-oxoglutarate dependent dioxygenase involved in the second-to-last step in vindoline biosynthesis

Plant Physiol.

1323-1329

1990

Catharanthus roseus

16667836

brenda

440242

De Carolis, E.; De Luca, V.

Purification, characterization, and kinetic analysis of a 2-oxoglutarate-dependent dioxygenase involved in vindoline biosynthesis from Catharanthus roseus

J. Biol. Chem.

268

5504-5511

1993

Catharanthus roseus

8449913

brenda

440243

Carolis, E.D.; Luca, V.D.

A novel 2-oxoglutarate-dependent dioxygenase involved in vindoline biosynthesis: Characterization, purification and kinetic properties

Plant Cell Tissue Organ Cult.

281-287

1994

Catharanthus roseus

brenda

Vazquez-Flota, F.; De Carolis, E.; Alarco, A.M.; De Luca, V.

Molecular cloning and characterization of desacetoxyvindoline-4-hydroxylase, a 2-oxoglutarate dependent-dioxygenase involved in the biosynthesis of vindoline in Catharanthus roseus (L.) G. Don

Plant Mol. Biol.

935-948

1997

Catharanthus roseus (O04847), Catharanthus roseus

9290645

brenda

440245

Vazquez-Flota, F.A.; De Luca, V.

Developmental and light regulation of desacetoxyvindoline 4-hydroxylase in Catharanthus roseus (L.) G. Don. Evidence of a multilevel regulatory mechanism

Plant Physiol.

117

1351-1361

1998

Catharanthus roseus (O04847), Catharanthus roseus

9701591

brenda

655109

Hernandez-Dominguez, E.; Campos-Tamayo, F.; Vazquez-Flota, F.

Vindoline synthesis in in vitro shoot cultures of Catharanthus roseus

Biotechnol. Lett.

671-674

2004

Catharanthus roseus

15200179

brenda

660317

Dutta, A.; Batra, J.; Pandey-Rai, S.; Singh, D.; Kumar, S.; Sen, J.

Expression of terpenoid indole alkaloid biosynthetic pathway genes corresponds to accumulation of related alkaloids in Catharanthus roseus (L.) G. Don

Planta

220

376-383

2005

Catharanthus roseus (O04847), Catharanthus roseus

15714355

brenda

684439

Campos-Tamayo, F.; Hernandez-Dominguez, E.; Vazquez-Flota, F.

Vindoline formation in shoot cultures of Catharanthus roseus is synchronously activated with morphogenesis through the last biosynthetic step

Ann. Bot.

102

409-415

2008

Catharanthus roseus, Catharanthus roseus (O04847)

18587132

brenda

Guirimand, G.; Guihur, A.; Poutrain, P.; Hericourt, F.; Mahroug, S.; St-Pierre, B.; Burlat, V.; Courdavault, V.

Spatial organization of the vindoline biosynthetic pathway in Catharanthus roseus

J. Plant Physiol.

168

549-557

2010

Catharanthus roseus

21047699

brenda

Liu, J.; Zhu, J.; Tang, L.; Wen, W.; Lv, S.; Yu, R.

Enhancement of vindoline and vinblastine production in suspension-cultured cells of Catharanthus roseus by artemisinic acid elicitation

World J. Microbiol. Biotechnol.

175-180

2013

Catharanthus roseus

23864440

brenda

743812

Pandey, S.S.; Singh, S.; Babu, C.S.; Shanker, K.; Srivastava, N.K.; Shukla, A.K.; Kalra, A.

Fungal endophytes of Catharanthus roseus enhance vindoline content by modulating structural and regulatory genes related to terpenoid indole alkaloid biosynthesis

Sci. Rep.

26583

2016

Catharanthus roseus (O04847), Catharanthus roseus

27220774

brenda