Information on EC 1.13.99.B1 - exo-cleaving rubber dioxygenase

for references in articles please use BRENDA:EC1.13.99.B1
Word Map on EC 1.13.99.B1
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.13.99.B1
-
RECOMMENDED NAME
GeneOntology No.
exo-cleaving rubber dioxygenase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cis-1,4-polyisoprene + n O2 = tetra-C20 and higher oligo-isoprenoids with aldehyde and keto end groups
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
cis-1,4-polyisoprene:oxygen dioxygenase [oligo-isoprenoids with aldehyde and keto end groups forming]
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
-
A0A1S6Q8F9
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
A0A1S6Q8F9;
synergistic effect of the simultaneous presence of RoxAXsp and RoxBXsp on the efficiency of polyisoprene cleavage
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
cis-1,4-polyisoprene + n O2
?
show the reaction diagram
additional information
?
-
no substrates: squalene (1,4-trans-isoprenoid), carotenoids, or alpha-tocopherol
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
absence of covalently attached heme groups
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Iron
presence of one atom of iron per Lcp molecule. Purified Lcp as isolated contains an oxidized heme-Fe3+ that is free of bound dioxygen
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha-tocopherol
1 mM, 40% inhibition
Diethyl dithiocarbamate
diethyl thiocarbamate
10 mM, 56% inhibition
-
dithiothreitol
strong inhibition
imidazole
Pyridine
A0A1S6Q8F9;
-
squalene
1 mM, 10% inhibition
Triton X-100
0.1%, no residual activity
Tween 20
0.1%, no residual activity
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.7
22°C, pH 7.0
1.5
pH 7.0, 23°C
4.5
A0A1S6Q8F9;
pH 7.0, 23°C
5.7
A0A1S6Q8F9;
pH 7.0, 30°C
6.4
A0A1S6Q8F9;
pH 7.0, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
A0A1S6Q8F9;
calculated molecular mass is 73800 Da for precursor protein, and 70300 Da for mature protein
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2
22 h, 5% loss of activity
22
22 h, 70% loss of activity
37
22 h, 80% loss of activity
61.5
melting point
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
from a recombinant Xanthomonas sp. 35Y RoxA deletion mutant
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression inXanthomonas sp. strain 35Y
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression is upregulated during growth on polyisoprene
A0A1S6Q8F9;
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
degradation
A0A1S6Q8F9;
enzyme exerts a synergistic effect on the efficiency of polyisoprene cleavage by rubber oxidase RoxA, EC 1.13.11.85