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Synonyms
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nonheme bromoperoxidase
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nonheme bromoperoxidase
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RH + HBr + H2O2 = RBr + 2 H2O
Brings about the bromination of a range of organic molecules, forming stable C-Br bonds. Contains Fe(III), Zn(II), Ni(II), Co(II), but neither Vanadium nor heme
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bromide:hydrogen-peroxide oxidoreductase (metal-containing)
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Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione
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I- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione
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additional information
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Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione
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Substrates: i.e. monochlorodimedone
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Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione
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Substrates: i.e. monochlorodimedone
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I- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione
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Substrates: i.e. monochlorodimedone
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I- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione
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Substrates: i.e. monochlorodimedone
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additional information
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Substrates: inactive toward C1- and F- in the monochlorodimedone assay system
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additional information
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Substrates: inactive toward C1- and F- in the monochlorodimedone assay system
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additional information
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contains no heme
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Co2+
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contains 0.35 mol of cobalt ions per mol of enzyme. Addition of 0.1 mM CoCl2 results in 2.1fold increase in activity
Fe3+
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contains 2.0 mol of ferric iron per mol of enzyme
Ni2+
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contains 1.0 mol of nickel ions per mol of enzyme
Zn2+
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contains 0.8 mol of zinc ions per mol of enzyme
additional information
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contains no vanadium ions
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2-mercaptoethanol
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0.1 mM, 97% inhibition
FeCl3
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0.1 mM, 45% inhibition
KCN
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0.1 mM, 82% inhibition
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IF-3 strain
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IF-3 strain
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Highest Expressing Human Cell Lines
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dimer
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2 * 33000
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3.4
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2 h, 25°C, complete loss of activity below pH 3.5
672311
4 - 11
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2 h, 25°C, stable
672311
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0 - 20
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pH 7.0, 20 min, stable
30 - 35
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pH 7.0, 20 min, 10% loss of activity
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pH 7.0, 20 min, 20% loss of activity
50 - 60
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pH 7.0, 20 min, 10% loss of activity
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Itoh, N.; Morinaga, N.; Kouzai, T.
Purification and characterization of a novel metal-containing nonheme bromoperoxidase from Pseudomonas putida
Biochim. Biophys. Acta
1207
208-216
1994
Pseudomonas putida, Pseudomonas putida IF-3
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