Information on EC 1.10.3.3 - L-ascorbate oxidase

Reaction Schemes

hideshow

4

L-ascorbate

+

O2

=

4

monodehydroascorbate

+

2

H2O

4

+

=

4

+

2

Synonyms
ascorbate oxidase, ascorbic acid oxidase, l-ascorbate oxidase, aa-ox, af_ao1, aa oxidase, ascorbic oxidase, asc oxidase, l-ascorbic acid oxidase, l-ascorbate:oxygen oxidoreductase, more

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SYNONYM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

AA oxidase

-

-

-

-

AA-ox

2 entries

AAO

8 entries

AAO1

5 entries

AAO2

5 entries

AAO3

5 entries

Aao4

5 entries

Af_AO1

2 entries

AO1

Gossypium hirsutum

A0A1S5QMW4

-

742708, 743608

AO4

Cucumis sativus

-

isoform

743610

AOase

Cucurbita pepo

P37064

-

717986

ASC oxidase

Chaetomorpha linum

-

-

724853

ascorbase

-

-

-

-

ascorbate dehydrogenase

-

-

-

-

ascorbate oxidase

22 entries

ascorbic acid oxidase

6 entries

ascorbic oxidase

-

-

-

-

ASOM

2 entries

L-ascorbate:O2 oxidoreductase

-

-

-

-

L-ascorbic acid oxidase

-

-

-

-

oxidase, ascorbate

-

-

-

-

AA-ox

Cucurbita sp.

-

-

671319

AA-ox

Homo sapiens

-

-

765849

AAO

-

-

-

-

AAO

Brassica oleracea var. italica

-

-

712620

AAO

Chaetomorpha linum

-

-

724853

AAO

Cucurbita pepo

-

-

675660, 724962

AAO

Daucus carota

-

-

742547, 742548

AAO

Kalanchoe blossfeldiana

-

-

675660

AAO

Vigna unguiculata

-

-

725176

AAO

Zea mays

-

-

675660

AAO1

Arabidopsis thaliana

-

isoform

742602

AAO1

Glycine max

-

isoform

742602

AAO1

Oryza sativa

-

isoform

742602

AAO1

Sorghum bicolor

-

isoform

742602

AAO1

Zea mays

-

isoform

742602

AAO2

Arabidopsis thaliana

-

isoform

742602

AAO2

Glycine max

-

isoform

742602

AAO2

Oryza sativa

-

isoform

742602

AAO2

Sorghum bicolor

-

isoform

742602

AAO2

Zea mays

-

isoform

742602

AAO3

Arabidopsis thaliana

-

isoform

742602

AAO3

Glycine max

-

isoform

742602

AAO3

Oryza sativa

-

isoform

742602

AAO3

Sorghum bicolor

-

isoform

742602

AAO3

Zea mays

-

isoform

742602

Aao4

Arabidopsis thaliana

-

isoform

742602

Aao4

Glycine max

-

isoform

742602

Aao4

Oryza sativa

-

isoform

742602

Aao4

Sorghum bicolor

-

isoform

742602

Aao4

Zea mays

-

isoform

742602

Af_AO1

Aspergillus flavus

-

-

764908

Af_AO1

Aspergillus flavus ATCC 200026

-

-

-

ascorbate oxidase

-

-

-

-

ascorbate oxidase

Acremonium sp.

-

-

673049

ascorbate oxidase

Acremonium sp. HI-25

-

-

-

ascorbate oxidase

Aplysia californica

-

-

671318

ascorbate oxidase

Capsicum annuum

-

-

699312

ascorbate oxidase

Chaetomorpha linum

-

-

724853

ascorbate oxidase

Cucumis melo

Q5M5B4, Q5M5B5

-

676728

ascorbate oxidase

Cucumis sativus

P14133

-

699136

ascorbate oxidase

Cucurbita maxima

-

-

672768, 676611, 695699

ascorbate oxidase

Cucurbita pepo

-

-

724962

ascorbate oxidase

Cucurbita pepo

P37064

-

673569, 717986

ascorbate oxidase

Cucurbita sp.

-

-

671319, 696121

ascorbate oxidase

Hordeum vulgare

-

-

676487

ascorbate oxidase

Lagenaria siceraria

-

-

712792

ascorbate oxidase

Lotus japonicus

J7GBJ3

-

726244

ascorbate oxidase

Mangifera indica

-

-

699837

ascorbate oxidase

Nicotiana tabacum

-

-

675145

ascorbate oxidase

Pleurotus ostreatus

-

-

699504

ascorbate oxidase

Pleurotus ostreatus NFFA2

-

-

-

ascorbate oxidase

Psidium guajava

-

-

699837

ascorbate oxidase

Solanum lycopersicum

Q4VDN6

-

726138, 726244

ascorbate oxidase

Solanum lycopersicum West Virginia 106

Q4VDN6

-

-

ascorbic acid oxidase

-

-

-

-

ascorbic acid oxidase

Cucumis sativus

-

-

717286

ascorbic acid oxidase

Cucurbita pepo

-

-

675660

ascorbic acid oxidase

Kalanchoe blossfeldiana

-

-

675660

ascorbic acid oxidase

Vigna unguiculata

-

-

725176

ascorbic acid oxidase

Zea mays

-

-

675660

ASOM

Acremonium sp.

-

-

673049, 684895, 686374

ASOM

Acremonium sp. HI-25

-

-

-

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REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

4 L-ascorbate + O2 = 4 monodehydroascorbate + 2 H2O

8 entries

4 L-ascorbate + O2 = 4 monodehydroascorbate + 2 H2O

rate determining step in the catalytic mechanism may involve protonation of an intermediate

Cucurbita pepo medullosa

-

439923

4 L-ascorbate + O2 = 4 monodehydroascorbate + 2 H2O

intramolecular electron transfer from type I copper to type III copper is probably the rate-limiting step in enzyme catalysis

Cucurbita pepo medullosa

-

439900

4 L-ascorbate + O2 = 4 monodehydroascorbate + 2 H2O

multiple displacement mechanism, enzyme exhibits enzymic memory

Cucurbita pepo condensa

-

439916

4 L-ascorbate + O2 = 4 monodehydroascorbate + 2 H2O

mechanism: Cu2+ is reduced to Cu+, which is then reoxidized by oxygen

Cucurbita pepo condensa

-

439889

4 L-ascorbate + O2 = 4 monodehydroascorbate + 2 H2O

binding mode of phenolic inhibitors and substrate to active site, binding site in a pocket near type I copper center

Cucurbita pepo

-

655740

4 L-ascorbate + O2 = 4 monodehydroascorbate + 2 H2O

mechanism and rate constants

Cucurbita pepo medullosa

-

656442

4 L-ascorbate + O2 = 4 monodehydroascorbate + 2 H2O

the transition from native dimer to the dimeric intermediate is characterized by the release of copper ions forming the tri-nuclear copper center located at the interface between domain 2 and 3 of each subunit

Cucurbita pepo

P37064

673569

4 L-ascorbate + O2 = 4 monodehydroascorbate + 2 H2O

-

-

-

-

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REACTION TYPE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

oxidation

-

-

-

-

redox reaction

-

-

-

-

reduction

-

-

-

-

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PATHWAY SOURCE

PATHWAYS

KEGG

Ascorbate and aldarate metabolism

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SYSTEMATIC NAME

IUBMB Comments

L-ascorbate:oxygen oxidoreductase

A multicopper protein.

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CAS REGISTRY NUMBER

COMMENTARY

9029-44-1

-

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SUBSTRATE

PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

Reversibility
r=reversible
ir=irreversible
?=not specified

2 hydroquinone + O2

2 quinone + 2 H2O

Cucurbita pepo condensa

-

439889

Substrates: at pH 7.2
Products: -

?

2 L-ascorbate + O2

2 L-dehydroascorbate + 2 H2O

17 entries

2 L-ascorbate + O2 + 4 H+

2 monodehydroascorbate + 2 H2O

2 entries

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

22 entries

2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2

?

2 entries

2,5-dichlorohydroquinone + O2

2,5-dichloroquinone + H2O

2 entries

2,6-dichlorohydroquinone + O2

2,6-dichloroquinone + H2O

2 entries

2,6-dichloroindophenol + O2

oxidized 2,6-dichloroindophenol + H2O

3 entries

4 L-ascorbate + O2

4 monodehydroascorbate + 2 H2O

11 entries

5,6-ene-L-ascorbate + O2

5,6-ene-L-dehydroascorbate

Cucurbita pepo medullosa

-

439931

Substrates: -
Products: -

?

5,6-isopropylidene-L-ascorbate + O2

5,6-isopropylidene-L-dehydroascorbate

Cucurbita pepo medullosa

-

439931

Substrates: -
Products: -

?

5-methyl-5-O-(alpha-D-glucopyranosyl)-D-erythroascorbic acid + O2

? + H2O2

2 entries

5-methyl-5-O-(alpha-D-xylopyranosyl)-D-erythroascorbic acid + O2

? + H2O2

2 entries

5-O-(alpha-D-glucopyranosyl)-D-erythroascorbic acid + O2

? + H2O2

2 entries

5-O-(alpha-D-xylopyranosyl)-D-erythroascorbic acid + O2

? + H2O2

2 entries

6-amino-L-ascorbate + O2

6-amino-L-dehydroascorbate

Cucurbita pepo medullosa

-

439931

Substrates: -
Products: -

?

6-bromo-L-ascorbate + O2

6-bromo-L-dehydroascorbate

Cucurbita pepo medullosa

-

439931

Substrates: -
Products: -

?

6-deoxy-L-ascorbate + O2

6-deoxy-L-dehydroascorbate

Cucurbita pepo medullosa

-

439931

Substrates: -
Products: -

?

6-O-phenyl-L-ascorbate + O2

6-O-phenyl-L-dehydroascorbate

Cucurbita pepo medullosa

-

439931

Substrates: -
Products: -

?

6-S-phenyl-L-ascorbate + O2

6-S-phenyl-L-dehydroascorbate

Cucurbita pepo medullosa

-

439931

Substrates: -
Products: -

?

chlorohydroquinone + O2

chloroquinone + H2O

Cucurbita sp.

-

439928

Substrates: 6% of activity with L-ascorbic acid
Products: -

?

D-erythroascorbic acid + O2

? + H2O2

Pleurotus ostreatus

-

699504

Substrates: -
Products: -

?

D-glucoascorbic acid + O2

D-glucodehydroascorbate + H2O

Cucurbita pepo condensa

-

439889

Substrates: -
Products: -

?

D-isoascorbate + O2

2-dehydroisoascorbate + H2O

2 entries

D-isoascorbate + O2

?

Cucurbita pepo medullosa

-

439931

Substrates: -
Products: -

?

D-isoascorbic acid + O2

2-dehydroisoascorbate + H2O

4 entries

hydroxyhydroquinone + O2

hydroxyquinone + H2O

Cucurbita pepo condensa

-

439926

Substrates: oxidation rate approx. 1/12 that of ascorbic acid, pH 5.7
Products: -

?

L-ascorbate + O2

monodehydroascorbate + H2O

11 entries

L-ascorbyl-6-palmitate + O2

L-dehydroascorbyl-6-palmitate

Acremonium

-

439932

Substrates: 36% of activity with L-ascorbate
Products: -

?

L-ascorbyl-6-stearate + O2

L-dehydroascorbyl-6-stearate

Acremonium

-

439932

Substrates: 29% of activity with L-ascorbate
Products: -

?

leuco 2,6-dichloroindophenol + O2

oxidized leuco 2,6-dichloroindophenol + H2O

Cucurbita pepo condensa

-

439926

Substrates: oxidation to the blue quinoid dye at pH 5.7
Products: -

?

additional information

?

-

9 entries

2 L-ascorbate + O2

2 L-dehydroascorbate + 2 H2O

Albifimbria verrucaria

-

396331

Substrates: highly specific for ascorbic acid and a few of its analogs and O2
Products: -

?

2 L-ascorbate + O2

2 L-dehydroascorbate + 2 H2O

Citrus unshiu

-

439930

Substrates: -
Products: -

?

2 L-ascorbate + O2

2 L-dehydroascorbate + 2 H2O

Cucumis sativus

-

439890, 439924

Substrates: -
Products: -

?

2 L-ascorbate + O2

2 L-dehydroascorbate + 2 H2O

Cucumis sativus

P14133

699136

Substrates: -
Products: -

?

2 L-ascorbate + O2

2 L-dehydroascorbate + 2 H2O

Cucumis sativus

-

391025

Substrates: low reaction rate with bilirubin
Products: -

?

2 L-ascorbate + O2

2 L-dehydroascorbate + 2 H2O

Cucurbita pepo condensa

-

439890, 439924

Substrates: -
Products: -

?

2 L-ascorbate + O2

2 L-dehydroascorbate + 2 H2O

Cucurbita pepo condensa

-

439916

Substrates: double displacement mechanism, so called enzymatic memory
Products: -

?

2 L-ascorbate + O2

2 L-dehydroascorbate + 2 H2O

Cucurbita pepo condensa

-

439926

Substrates: substrate specificity: overview
Products: -

?

2 L-ascorbate + O2

2 L-dehydroascorbate + 2 H2O

Cucurbita pepo condensa

-

439889

Substrates: no appreciable activity with: p-phenylenediamine, Na2S2O3, glutathione and cysteine
Products: -

?

2 L-ascorbate + O2

2 L-dehydroascorbate + 2 H2O

Cucurbita pepo condensa

-

439926

Substrates: anionic form of the substrate is an important requirement of the enzyme specificity
Products: -

?

2 L-ascorbate + O2

2 L-dehydroascorbate + 2 H2O

Cucurbita pepo condensa

-

439926

Substrates: no oxidation of hydroquinone
Products: -

?

2 L-ascorbate + O2

2 L-dehydroascorbate + 2 H2O

Cucurbita pepo medullosa

-

439890, 439910, 439919, 439931, 439937, 439939

Substrates: -
Products: -

?

2 L-ascorbate + O2

2 L-dehydroascorbate + 2 H2O

Cucurbita sp.

-

439928

Substrates: -
Products: -

?

2 L-ascorbate + O2

2 L-dehydroascorbate + 2 H2O

Cucurbita sp.

-

439940

Substrates: enzyme may play a key role in the regulation of cell expansion
Products: -

?

2 L-ascorbate + O2

2 L-dehydroascorbate + 2 H2O

Cucurbita sp.

-

439909

Substrates: possibly a kind of pathogenesis-related protein
Products: -

?

2 L-ascorbate + O2

2 L-dehydroascorbate + 2 H2O

Lagenaria siceraria

-

712792

Substrates: -
Products: -

?

2 L-ascorbate + O2

2 L-dehydroascorbate + 2 H2O

Triticum aestivum

-

439938

Substrates: -
Products: -

?

2 L-ascorbate + O2 + 4 H+

2 monodehydroascorbate + 2 H2O

Cucumis sativus

-

717286

Substrates: formation of free radicals during the oxidation reaction by ascorbate oxidase
Products: -

?

2 L-ascorbate + O2 + 4 H+

2 monodehydroascorbate + 2 H2O

Cucumis sativus

-

717286

Substrates: formation of free radicals during the oxidation reaction by ascorbate oxidase, determination of steady-state free radical concentration, overview
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Acremonium sp.

-

673049

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Acremonium sp. HI-25

-

673049

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Aplysia californica

-

671318

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Brassica oleracea var. italica

-

712620

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Citrullus lanatus

-

676456

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Citrullus lanatus

-

676456

Substrates: role of AO is regulation of cell division through controlled apoplastic Asc levels
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Cucumis melo

Q5M5B4, Q5M5B5

676728

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Cucumis melo

Q5M5B4, Q5M5B5

676728

Substrates: the gene expression is transcriptionally regulated during fruit development and in response to hormonal cues associated with the control of cell growth and the stress response
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Cucurbita maxima

-

439920, 672768, 676611, 695699

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Cucurbita maxima

-

676611

Substrates: metabolic regulation, overview
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Cucurbita pepo

-

675660

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Cucurbita pepo

P37064

673569

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Cucurbita pepo

-

675660

Substrates: the enzyme activity is up- or down-regulated in response to oxygen availability
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Hordeum vulgare

-

676487

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Hordeum vulgare

-

676487

Substrates: aerobic oxidation of ascorbate to the unstable ascorbate free radical
Products: the radical plays a role as an electron acceptor in plant growth including plasma membrane electron transport, cell elongation and cell wall metabolism, overview

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Kalanchoe blossfeldiana

-

675660

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Kalanchoe blossfeldiana

-

675660

Substrates: the enzyme activity is up- or down-regulated in response to oxygen availability
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Nicotiana tabacum

-

675145

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Pleurotus ostreatus

-

699504

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Pleurotus ostreatus NFFA2

-

699504

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Zea mays

-

675660

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Zea mays

-

675660

Substrates: the enzyme activity is up- or down-regulated in response to oxygen availability
Products: -

?

2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2

?

Aspergillus flavus

-

764908

Substrates: -
Products: -

?

2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2

?

Aspergillus flavus ATCC 200026

-

764908

Substrates: -
Products: -

?

2,5-dichlorohydroquinone + O2

2,5-dichloroquinone + H2O

Cucurbita pepo condensa

-

439889

Substrates: -
Products: -

?

2,5-dichlorohydroquinone + O2

2,5-dichloroquinone + H2O

Cucurbita pepo condensa

-

439926

Substrates: oxidation rate approx. 1/12 that of ascorbic acid, pH 5.7
Products: -

?

2,6-dichlorohydroquinone + O2

2,6-dichloroquinone + H2O

Cucurbita pepo condensa

-

439889

Substrates: -
Products: -

?

2,6-dichlorohydroquinone + O2

2,6-dichloroquinone + H2O

Cucurbita pepo condensa

-

439926

Substrates: oxidation rate approx. 1/12 that of ascorbic acid, pH 5.7
Products: -

?

2,6-dichloroindophenol + O2

oxidized 2,6-dichloroindophenol + H2O

Acremonium sp.

-

673049

Substrates: 2,6-dichloroindophenol is an effective electron-transfer mediator for the enzyme, while ferrocyanide and cytochrome c are poor mediators
Products: -

?

2,6-dichloroindophenol + O2

oxidized 2,6-dichloroindophenol + H2O

Acremonium sp. HI-25

-

673049

Substrates: 2,6-dichloroindophenol is an effective electron-transfer mediator for the enzyme, while ferrocyanide and cytochrome c are poor mediators
Products: -

?

2,6-dichloroindophenol + O2

oxidized 2,6-dichloroindophenol + H2O

Beta vulgaris subsp. vulgaris

A0A0J8EGV0

765763

Substrates: -
Products: -

?

4 L-ascorbate + O2

4 monodehydroascorbate + 2 H2O

Aspergillus flavus

-

764908

Substrates: -
Products: -

?

4 L-ascorbate + O2

4 monodehydroascorbate + 2 H2O

Aspergillus flavus ATCC 200026

-

764908

Substrates: -
Products: -

?

4 L-ascorbate + O2

4 monodehydroascorbate + 2 H2O

Beta vulgaris subsp. vulgaris

A0A0J8EGV0

765763

Substrates: -
Products: -

?

4 L-ascorbate + O2

4 monodehydroascorbate + 2 H2O

Chaetomorpha linum

-

724853

Substrates: -
Products: -

?

4 L-ascorbate + O2

4 monodehydroascorbate + 2 H2O

Cucurbita moschata

-

764566

Substrates: -
Products: -

?

4 L-ascorbate + O2

4 monodehydroascorbate + 2 H2O

Cucurbita pepo

-

724962, 764566

Substrates: -
Products: -

?

4 L-ascorbate + O2

4 monodehydroascorbate + 2 H2O

Cucurbita sp.

-

764784

Substrates: the enzyme is involved in catalyzing the oxidation of apoplastic ascorbic acid to dehydroascorbic acid
Products: -

?

4 L-ascorbate + O2

4 monodehydroascorbate + 2 H2O

Lotus japonicus

J7GBJ3

726204, 726244

Substrates: -
Products: -

?

4 L-ascorbate + O2

4 monodehydroascorbate + 2 H2O

Solanum lycopersicum

Q4VDN6

726138, 726244

Substrates: -
Products: -

?

4 L-ascorbate + O2

4 monodehydroascorbate + 2 H2O

Solanum lycopersicum West Virginia 106

Q4VDN6

726138

Substrates: -
Products: -

?

4 L-ascorbate + O2

4 monodehydroascorbate + 2 H2O

Vigna unguiculata

-

725176

Substrates: -
Products: -

?

5-methyl-5-O-(alpha-D-glucopyranosyl)-D-erythroascorbic acid + O2

? + H2O2

Pleurotus ostreatus

-

699504

Substrates: -
Products: -

?

5-methyl-5-O-(alpha-D-glucopyranosyl)-D-erythroascorbic acid + O2

? + H2O2

Pleurotus ostreatus NFFA2

-

699504

Substrates: -
Products: -

?

5-methyl-5-O-(alpha-D-xylopyranosyl)-D-erythroascorbic acid + O2

? + H2O2

Pleurotus ostreatus

-

699504

Substrates: -
Products: -

?

5-methyl-5-O-(alpha-D-xylopyranosyl)-D-erythroascorbic acid + O2

? + H2O2

Pleurotus ostreatus NFFA2

-

699504

Substrates: -
Products: -

?

5-O-(alpha-D-glucopyranosyl)-D-erythroascorbic acid + O2

? + H2O2

Pleurotus ostreatus

-

699504

Substrates: -
Products: -

?

5-O-(alpha-D-glucopyranosyl)-D-erythroascorbic acid + O2

? + H2O2

Pleurotus ostreatus NFFA2

-

699504

Substrates: -
Products: -

?

5-O-(alpha-D-xylopyranosyl)-D-erythroascorbic acid + O2

? + H2O2

Pleurotus ostreatus

-

699504

Substrates: -
Products: -

?

5-O-(alpha-D-xylopyranosyl)-D-erythroascorbic acid + O2

? + H2O2

Pleurotus ostreatus NFFA2

-

699504

Substrates: -
Products: -

?

D-isoascorbate + O2

2-dehydroisoascorbate + H2O

Cucurbita pepo medullosa

-

439931

Substrates: -
Products: -

r

D-isoascorbate + O2

2-dehydroisoascorbate + H2O

Triticum aestivum

-

439938

Substrates: -
Products: -

r

D-isoascorbic acid + O2

2-dehydroisoascorbate + H2O

Acremonium

-

439932

Substrates: -
Products: -

?

D-isoascorbic acid + O2

2-dehydroisoascorbate + H2O

Cucurbita pepo condensa

-

439889

Substrates: -
Products: -

?

D-isoascorbic acid + O2

2-dehydroisoascorbate + H2O

Cucurbita pepo medullosa

-

439931

Substrates: -
Products: -

?

D-isoascorbic acid + O2

2-dehydroisoascorbate + H2O

Triticum aestivum

-

439938

Substrates: -
Products: -

?

L-ascorbate + O2

monodehydroascorbate + H2O

Arabidopsis thaliana

-

742602

Substrates: -
Products: -

?

L-ascorbate + O2

monodehydroascorbate + H2O

Brassica oleracea

-

439893

Substrates: -
Products: -

r

L-ascorbate + O2

monodehydroascorbate + H2O

Cucumis sativus

-

743610

Substrates: -
Products: -

?

L-ascorbate + O2

monodehydroascorbate + H2O

Daucus carota

-

742547, 742548

Substrates: -
Products: -

?

L-ascorbate + O2

monodehydroascorbate + H2O

Glycine max

-

742602

Substrates: -
Products: -

?

L-ascorbate + O2

monodehydroascorbate + H2O

Gossypium hirsutum

A0A1S5QMW4

742708, 743608

Substrates: -
Products: -

?

L-ascorbate + O2

monodehydroascorbate + H2O

Gossypium hirsutum

-

743608

Substrates: -
Products: -

?

L-ascorbate + O2

monodehydroascorbate + H2O

Homo sapiens

-

743057

Substrates: -
Products: -

?

L-ascorbate + O2

monodehydroascorbate + H2O

Oryza sativa

-

742602

Substrates: -
Products: -

?

L-ascorbate + O2

monodehydroascorbate + H2O

Sorghum bicolor

-

742602

Substrates: -
Products: -

?

L-ascorbate + O2

monodehydroascorbate + H2O

Zea mays

-

742602

Substrates: -
Products: -

?

additional information

?

-

Acremonium sp.

-

673049

Substrates: the active site binding pocket of ascorbate oxidase is specific for ascorbate10 and the electron transfer of ascorbate oxidase is strongly dependent on the nature of the electron donor, overview
Products: -

?

additional information

?

-

Acremonium sp.

-

684895

Substrates: the oxidative activity of ascorbate oxidase is dominated by the highly selective substrate-binding affinity based on electrostatic interaction beyond the one-electron redox potential difference between type 1 copper site of ascorbate oxidase and substrate
Products: -

?

additional information

?

-

Acremonium sp. HI-25

-

673049

Substrates: the active site binding pocket of ascorbate oxidase is specific for ascorbate10 and the electron transfer of ascorbate oxidase is strongly dependent on the nature of the electron donor, overview
Products: -

?

additional information

?

-

Acremonium sp. HI-25

-

684895

Substrates: the oxidative activity of ascorbate oxidase is dominated by the highly selective substrate-binding affinity based on electrostatic interaction beyond the one-electron redox potential difference between type 1 copper site of ascorbate oxidase and substrate
Products: -

?

additional information

?

-

Aplysia californica

-

671318

Substrates: ascorbic acid interferes with the realtime measurement of neuronal NO, an important gaseous neurotransmitter, by use of diaminofluoresceins, which act as receptors for NO, ascorbic acid reduces N2O3, the reaction intermediate of NO oxidation, while dehydroascorbic acid competes with NO for the receptors, while ascorbic acid inhibits the binding between NO and diaminofluoresceins, overview
Products: -

?

additional information

?

-

Aspergillus flavus

-

764908

Substrates: syringaldezine and guaiacol are not oxidized
Products: -

-

additional information

?

-

Aspergillus flavus ATCC 200026

-

764908

Substrates: syringaldezine and guaiacol are not oxidized
Products: -

-

additional information

?

-

Cucumis sativus

-

717286

Substrates: the enzyme might perform two types of free radical decay reaction, dismutation and oxidation
Products: -

?

additional information

?

-

Nicotiana tabacum

-

675145

Substrates: cell wall-localized enzyme that catalyses the oxidation of ascorbate to the unstable radical monodehydroascorbate, which rapidly disproportionates to yield dehydroascorbate and ascorbate, and thus contributes to the regulation of the ascorbic acid redox state regulating the cell redox status
Products: -

?

top print hide show all columns Go to Natural Substrate Search

NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

2 L-ascorbate + O2

2 L-dehydroascorbate + 2 H2O

2 entries

2 L-ascorbate + O2 + 4 H+

2 monodehydroascorbate + 2 H2O

Cucumis sativus

-

717286

Substrates: formation of free radicals during the oxidation reaction by ascorbate oxidase
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

13 entries

4 L-ascorbate + O2

4 monodehydroascorbate + 2 H2O

7 entries

L-ascorbate + O2

monodehydroascorbate + H2O

10 entries

additional information

?

-

Nicotiana tabacum

-

675145

Substrates: cell wall-localized enzyme that catalyses the oxidation of ascorbate to the unstable radical monodehydroascorbate, which rapidly disproportionates to yield dehydroascorbate and ascorbate, and thus contributes to the regulation of the ascorbic acid redox state regulating the cell redox status
Products: -

?

2 L-ascorbate + O2

2 L-dehydroascorbate + 2 H2O

Cucurbita sp.

-

439940

Substrates: enzyme may play a key role in the regulation of cell expansion
Products: -

?

2 L-ascorbate + O2

2 L-dehydroascorbate + 2 H2O

Cucurbita sp.

-

439909

Substrates: possibly a kind of pathogenesis-related protein
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Acremonium sp.

-

673049

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Acremonium sp. HI-25

-

673049

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Aplysia californica

-

671318

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Citrullus lanatus

-

676456

Substrates: role of AO is regulation of cell division through controlled apoplastic Asc levels
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Cucumis melo

Q5M5B4, Q5M5B5

676728

Substrates: the gene expression is transcriptionally regulated during fruit development and in response to hormonal cues associated with the control of cell growth and the stress response
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Cucurbita maxima

-

672768, 695699

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Cucurbita maxima

-

676611

Substrates: metabolic regulation, overview
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Cucurbita pepo

P37064

673569

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Cucurbita pepo

-

675660

Substrates: the enzyme activity is up- or down-regulated in response to oxygen availability
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Hordeum vulgare

-

676487

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Kalanchoe blossfeldiana

-

675660

Substrates: the enzyme activity is up- or down-regulated in response to oxygen availability
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Nicotiana tabacum

-

675145

Substrates: -
Products: -

?

2 L-ascorbic acid + O2

2 L-dehydroascorbic acid + 2 H2O

Zea mays

-

675660

Substrates: the enzyme activity is up- or down-regulated in response to oxygen availability
Products: -

?

4 L-ascorbate + O2

4 monodehydroascorbate + 2 H2O

Chaetomorpha linum

-

724853

Substrates: -
Products: -

?

4 L-ascorbate + O2

4 monodehydroascorbate + 2 H2O

Cucurbita pepo

-

724962

Substrates: -
Products: -

?

4 L-ascorbate + O2

4 monodehydroascorbate + 2 H2O

Cucurbita sp.

-

764784

Substrates: the enzyme is involved in catalyzing the oxidation of apoplastic ascorbic acid to dehydroascorbic acid
Products: -

?

4 L-ascorbate + O2

4 monodehydroascorbate + 2 H2O

Lotus japonicus

J7GBJ3

726204, 726244

Substrates: -
Products: -

?

4 L-ascorbate + O2

4 monodehydroascorbate + 2 H2O

Solanum lycopersicum

Q4VDN6

726138, 726244

Substrates: -
Products: -

?

4 L-ascorbate + O2

4 monodehydroascorbate + 2 H2O

Solanum lycopersicum West Virginia 106

Q4VDN6

726138

Substrates: -
Products: -

?

4 L-ascorbate + O2

4 monodehydroascorbate + 2 H2O

Vigna unguiculata

-

725176

Substrates: -
Products: -

?

L-ascorbate + O2

monodehydroascorbate + H2O

Arabidopsis thaliana

-

742602

Substrates: -
Products: -

?

L-ascorbate + O2

monodehydroascorbate + H2O

Cucumis sativus

-

743610

Substrates: -
Products: -

?

L-ascorbate + O2

monodehydroascorbate + H2O

Daucus carota

-

742547, 742548

Substrates: -
Products: -

?

L-ascorbate + O2

monodehydroascorbate + H2O

Glycine max

-

742602

Substrates: -
Products: -

?

L-ascorbate + O2

monodehydroascorbate + H2O

Gossypium hirsutum

A0A1S5QMW4

742708, 743608

Substrates: -
Products: -

?

L-ascorbate + O2

monodehydroascorbate + H2O

Gossypium hirsutum

-

743608

Substrates: -
Products: -

?

L-ascorbate + O2

monodehydroascorbate + H2O

Homo sapiens

-

743057

Substrates: -
Products: -

?

L-ascorbate + O2

monodehydroascorbate + H2O

Oryza sativa

-

742602

Substrates: -
Products: -

?

L-ascorbate + O2

monodehydroascorbate + H2O

Sorghum bicolor

-

742602

Substrates: -
Products: -

?

L-ascorbate + O2

monodehydroascorbate + H2O

Zea mays

-

742602

Substrates: -
Products: -

?

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COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

heme

Pleurotus ostreatus

-

-

699504

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Co2+

Gossypium hirsutum

A0A1S5QMW4

contains copper

742708

copper

25 entries

Cu2+

10 entries

NaCl

Chaetomorpha linum

-

induces the ascorbate oxidase activity

724853

additional information

2 entries

copper

Acremonium

-

-

439932

copper

Acremonium

-

enzyme contains type I, type II and type III copper atoms in the ratio 1/2/2, 4 copper atoms/enzyme

439929

copper

Acremonium sp.

-

the oxidative activity of ascorbate oxidase is dominated by the highly selective substrate-binding affinity based on electrostatic interaction beyond the one-electron redox potential difference between type 1 copper site of ascorbate oxidase and substrate

684895

copper

Aspergillus flavus

-

multi-copper oxidase

764908

copper

Cucumis sativus

-

10 copper atoms per enzyme molecule

439890

copper

Cucumis sativus

-

4 copper atoms per subunit, mononuclear blue copper in domain 3 and trinuclear copper between domain 1 and 3

439899

copper

Cucumis sativus

-

principal active site comprised of one type I, one type II and a pair of type III coppers

439917

copper

Cucumis sativus

-

enzyme contains a set of 1 type I, 1 type II and a pair of type III copper ions at its active site

439917

copper

Cucumis sativus

-

8 atoms of copper per enzyme molecule of 132000 Da

439894

copper

Cucumis sativus

-

8 atoms of copper per enzyme molecule of 140000 Da

439922

copper

Cucurbita pepo condensa

-

-

439890

copper

Cucurbita pepo condensa

- <