Information on EC 1.1.3.B4 - glycerol oxidase

for references in articles please use BRENDA:EC1.1.3.B4
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The enzyme appears in viruses and cellular organisms

top print hide
EC NUMBER
COMMENTARY hide
1.1.3.B4
preliminary BRENDA-supplied EC number
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RECOMMENDED NAME
GeneOntology No.
glycerol oxidase
-
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
glycerol + O2 = glyceraldehyde + H2O2
show the reaction diagram
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
glycerol:oxygen 1-oxidoreductase
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,3-butanediol + O2
?
show the reaction diagram
1,3-propanediol + O2
?
show the reaction diagram
-
-
-
-
?
1,4-butanediol + O2
?
show the reaction diagram
D-fructose + O2
?
show the reaction diagram
D-galactose + O2
?
show the reaction diagram
dihydroxyacetone + O2
3-hydroxy-2-oxopropanal + H2O2
show the reaction diagram
glycerol + O2
glyceraldehyde + H2O2
show the reaction diagram
additional information
?
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glycerol + O2
glyceraldehyde + H2O2
show the reaction diagram
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
protoheme IX
-
the enzyme contains 0.94 mol of heme per mol of enzyme
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
contains 2 atoms of copper per mol of enzyme
Cu2+
-
15% increase of activity in the presence of 1 mM Cu2+
Fe3+
-
10% increase of activity in the presence of 1 mM Fe3+
Mg2+
-
activates
Mn2+
-
activates
Ni2+
-
activates
additional information
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
25% inhibition at 0.01 mM
8-hydroxyquinoline
-
7.9% inhibition at 1 mM
Chloramine T
-
60% inhibition at 1 mM
diethyldithiocarbamate
-
40.1% inhibition at 1 mM
dimercapto-1-propanol
-
56.3% inhibition at 0.01 mM
dithiothreitol
-
26.5% inhibition at 0.01 mM
ethyl xanthate
-
27.1% inhibition at 0.1 mM
Hg2+
-
60% inhibition at 1 mM
hydroxylamine
-
87% inhibition at 1 mM
KCN
-
complete inhibition at 1 mM
L-ascorbic acid
-
31.2% inhibition at 0.1 mM
L-cysteine
-
12.2% inhibition at 0.1 mM
NaN3
-
complete inhibition at 1 mM
reduced glutathione
-
40.8% inhibition at 0.1 mM
Salicylaldoxime
-
11.2% inhibition at 1 mM
sodium sulfite
-
46.2% inhibition at 0.1 mM
Tetranitromethane
-
46% inhibition at 1 mM
additional information
-
EDTA, phenylglycoxyl, iodoacetic acid, and o-phenanthroline do not show significant inhibitory effects on the enzyme activity
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Borate
-
exposure of the enzyme to borate buffer of pH 10.0 results in activation of the enzyme
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.9
glycerol
at pH 9.0 and 50°C
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.012
dimercapto-1-propanol
-
at pH 7.0 and 37°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.7
-
crude extract, at pH 7.0 and 30°C
66.7
-
after 39.2fold purification, at pH 7.0 and 30°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7
-
-
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 10
more than 60% activity between pH 7.0 and 10.0
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.4
isoelectric focusing
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
75000
2 * 75000, SDS-PAGE
400000
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
2 * 75000, SDS-PAGE
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 6.5
-
the enzyme is stable in the pH range from 5.5 to 6.5
738126
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 60
-
after 1 h of incubation, the enzyme is relatively stable from 20 to 40°C and loses most of its activity over 50°C
45 - 50
-
the enzyme is stable up to 45°C and loses about 40% of activity at 50°C in 50 mM TES buffer, pH 7.0
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
5°C, activated enzyme at pH 8.0-10.0, 1 week, no loss of activity
-
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate fractionation, Sephadex G-100 gel filtration, Fractogel DEAE-650M column chromatography, Toyopearl phenyl-650M column chromatography, and Ultrogel hydroxyapatite column chromatography
-
DEAE-Sephadex A-25 column chromatography, hydroxylapatite column chromatography, and Sephadex G-200 gel filtration
-
Superdex 75 gel filtration
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LINKS TO OTHER DATABASES (specific for EC-Number 1.1.3.B4)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)