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D-galactose + O2 = D-galacto-hexodialdose + H2O2
D-galactose + O2 = D-galacto-hexodialdose + H2O2
-
-
-
-
D-galactose + O2 = D-galacto-hexodialdose + H2O2
mechanism
-
D-galactose + O2 = D-galacto-hexodialdose + H2O2
detailed mechanism, selective for pro-S hydrogen abstraction
-
D-galactose + O2 = D-galacto-hexodialdose + H2O2
detailed reaction mechanism, the active site of galactose oxidase bears a Cu2+ with an inner coordination sphere involving Tyr272, Tyr495, His496, His581, and a coordinated solvent molecule, and Trp290 in the outer sphere of the complex, overview
-
D-galactose + O2 = D-galacto-hexodialdose + H2O2
reaction mechanism, structure-function analysis, two-electron redox reaction involving a Cu(I)/Cu(II) couple and the reversible oxidation of a ligating phenolate, tyrosine residue of the Tyr272-Cys228 conjugate, to a phenoxyl radical, overview
-
D-galactose + O2 = D-galacto-hexodialdose + H2O2
the active site consists of two one-electron redox units involving residues Y495, H496, H581, Y272, and W290, a Cu2+ ion, and a crosslinked Y272-C228 radical cofactor, which together are responsible for the catalytic activity, structure modelling
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D-galactose + O2 = D-galacto-hexodialdose + H2O2
oxidative and reductive half-reactions in the enzymatic cycle of galactose oxidase during oxidation of the C-6 hydroxyl group of D-galactose to the corresponding aldehyde
-
D-galactose + O2 = D-galacto-hexodialdose + H2O2
oxidative and reductive half-reactions in the enzymatic cycle of galactose oxidase during oxidation of the C-6 hydroxyl group of D-galactose to the corresponding aldehyde
D-galactose + O2 = D-galacto-hexodialdose + H2O2
oxidative and reductive half-reactions in the enzymatic cycle of galactose oxidase during oxidation of the C-6 hydroxyl group of D-galactose to the corresponding aldehyde
D-galactose + O2 = D-galacto-hexodialdose + H2O2
oxidative and reductive half-reactions in the enzymatic cycle of galactose oxidase during oxidation of the C-6 hydroxyl group of D-galactose to the corresponding aldehyde
D-galactose + O2 = D-galacto-hexodialdose + H2O2
oxidative and reductive half-reactions in the enzymatic cycle of galactose oxidase during oxidation of the C-6 hydroxyl group of D-galactose to the corresponding aldehyde
-
D-galactose + O2 = D-galacto-hexodialdose + H2O2
oxidative and reductive half-reactions in the enzymatic cycle of galactose oxidase during oxidation of the C-6 hydroxyl group of D-galactose to the corresponding aldehyde
-
D-galactose + O2 = D-galacto-hexodialdose + H2O2
oxidative and reductive half-reactions in the enzymatic cycle of galactose oxidase during oxidation of the C-6 hydroxyl group of D-galactose to the corresponding aldehyde
-
D-galactose + O2 = D-galacto-hexodialdose + H2O2
the catalytic mechanism of enzyme GOase can be described by the ping-pong bi bi mechanism, where the alcohol substrate is oxidized in one catalytic halfreaction followed by reoxidation of the enzyme by reduction of oxygen to hydrogen peroxide in a second half-reaction
-
D-galactose + O2 = D-galacto-hexodialdose + H2O2
the first half-reaction involves proton transfer from O-6 of galactose to the axial tyrosine anion, hydrogen atom transfer (HAT) from C-6 of galactose to the tyrosine-cysteine radical cofactor and electron transfer from the carbohydrate to generate the aldehyde and Cu(I). To complete the catalytic cycle, the second half-reaction is proposed to involve inner-sphere electron transfer from Cu(I) to oxygen to yield superoxide, HAT from the phenolic hydroxyl group of the Tyr-Cys cofactor to the superoxide to produce metal-bound hydroperoxide and proton transfer from the axial tyrosine to hydroperoxide to produce hydrogen peroxide and the re-oxidized, active Cu(II)-radical state of the enzyme
D-galactose + O2 = D-galacto-hexodialdose + H2O2
the catalytic mechanism of enzyme GOase can be described by the ping-pong bi bi mechanism, where the alcohol substrate is oxidized in one catalytic halfreaction followed by reoxidation of the enzyme by reduction of oxygen to hydrogen peroxide in a second half-reaction
-
-
D-galactose + O2 = D-galacto-hexodialdose + H2O2
oxidative and reductive half-reactions in the enzymatic cycle of galactose oxidase during oxidation of the C-6 hydroxyl group of D-galactose to the corresponding aldehyde
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(furan-2-yl)methanol + O2
furan-2-carbaldehyde + H2O2
-
-
-
?
1,5-anhydrogalactitol + O2
? + H2O2
Polyporus circinatus
-
-
-
?
1-methyl-alpha-D-galactopyranoside + O2
1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
1-methyl-alpha-D-galactopyranoside + O2
? + H2O2
in the oxidations of methyl-alpha-D-galactopyranoside and methyl-beta-D-galactopyranoside, a dimeric product, a water elimination product, and an alpha,beta-unsaturated aldehyde occur among the mix of products. In the case of oxidized beta-galactose, the unsaturated aldehyde likely forms in the reaction
-
-
?
1-methyl-beta-D-galactopyranoside + O2
? + H2O2
1-O-methyl-alpha-D-galactopyranoside + O2
1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
-
-
-
-
?
1-O-methyl-alpha-D-galactosylpyranoside + O2
1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
1-O-methyl-alpha-D-glucosylpyranoside + O2
1-O-methyl-alpha-D-gluco-hexodialdose + H2O2
1-O-methyl-beta-D-galactosylpyranoside + O2
1-O-methyl-beta-D-galacto-hexodialdose + H2O2
1-O-methyl-beta-D-glucosylpyranoside + O2
1-O-methyl-beta-D-gluco-hexodialdose + H2O2
1-O-methyl-D-galactopyranoside + O2
?
-
112% of the activity with D-galactose
-
-
?
2 raffinose + 2 O2
6''-aldehydoraffinose + 6''-carboxyraffinose + H2O2 + H2O
2-deoxy-D-galactose + O2
2-deoxy-D-galacto-hexodialdose + H2O2
2-deoxy-D-galactose + O2
?
-
-
-
?
2-ethynylglycerol + O2
(2R)-2-ethynylglyceraldehyde + H2O2
-
-
-
?
2-glycerol-alpha-D-galactosylpyranoside + O2
2-glycerol-alpha-D-galactosyl-hexodialdose + H2O2
Polyporus circinatus
-
low activity
-
?
2-methylene-1,3-propanediol + O2
?
-
-
-
?
3-bromo-1,2-propanediol + O2
? + H2O2
-
-
S isomer
?
3-bromobenzyl alcohol + O2
3-bromobenzaldehyde + H2O2
-
-
-
?
3-chloro-1,2-propanediol + O2
? + H2O2
-
only R isomer will have the correct orientation to react with the enzyme
S isomer
?
3-chlorobenzyl alcohol + O2
3-chlorobenzaldehyde + H2O2
-
-
-
?
3-fluoro-1-phenylethanol + O2
?
-
-
-
-
?
3-fluorobenzyl alcohol + O2
3-fluorobenzaldehyde + H2O2
-
-
-
?
3-methoxybenzyl alcohol + O2
3-methoxybenzaldehyde + H2O2
-
-
-
?
3-methoxybenzyl alcohol + O2
? + H2O2
-
-
-
-
?
3-nitrobenzyl alcohol + O2
3-nitrobenzaldehyde + H2O2
-
-
-
?
4-(methylthio)benzyl alcohol + O2
4-(methylthio)benzaldehyde + H2O2
-
-
-
?
4-(trifluoromethyl)benzyl alcohol + O2
4-(trifluoromethyl)benzaldehyde + H2O2
-
-
-
?
4-bromobenzyl alcohol + O2
4-bromobenzaldehyde + H2O2
-
-
-
?
4-chlorobenzyl alcohol + O2
4-chlorobenzaldehyde + H2O2
-
-
-
?
4-fluorobenzyl alcohol + O2
4-fluorobenzaldehyde + H2O2
-
-
-
?
4-iodobenzyl alcohol + O2
4-iodobenzaldehyde + H2O2
-
-
-
?
4-methoxybenzyl alcohol + O2
4-methoxybenzaldehyde + H2O2
-
-
-
?
4-methoxybenzyl alcohol + O2
? + H2O2
-
-
-
-
?
4-methylbenzyl alcohol + O2
4-methylbenzaldehyde + H2O2
-
-
-
?
4-nitrobenzyl alcohol + O2
4-nitrobenzaldehyde + H2O2
-
-
-
?
4-nitrobenzyl alcohol + O2
? + H2O2
-
-
-
-
?
4-O-beta-D-glucopyranosyl-D-glucose + O2
4-O-beta-D-glucopyranosyl-D-gluco-hexodialdose + H2O2
5-(hydroxymethyl)furan-2-carbaldehyde + O2
furan-2,5-dicarbaldehyde + H2O2
-
-
-
?
acetol + O2
pyruvaldehyde + H2O2
-
-
-
?
allyl alcohol + O2
acrolein + H2O2
-
only extracelluar enzyme, low activity
-
?
alpha-D-melibiose + O2
? + H2O2
-
-
-
?
alpha-D-talose + O2
alpha-D-talo-hexodialdose + H2O2
benzene-1,2-diamine + O2
? + H2O2
-
very low activity
-
?
benzyl alcohol + O2
? + H2O2
benzyl alcohol + O2
benzaldehyde + H2O2
benzylalcohol + O2
benzaldehyde + H2O2
-
substrate reaction profiling
-
-
?
beta-D-galactopyranosyl-(1-6)-beta-D-galactopyranosyl-(1-4)-D-glucose + O2
? + H2O2
Polyporus circinatus
-
-
-
?
beta-D-galactosyl-(1-6)-beta-D-galactopyranoside + O2
? + H2O2
Polyporus circinatus
-
-
-
?
beta-D-lactose + O2
beta-D-lacto-hexodialdose + H2O2
beta-hydroxypyruvate + O2
2,3-dioxopropionate + H2O2
-
only extracellular enzyme, low activity
-
?
beta-thiodigalactoside + O2
? + H2O2
Polyporus circinatus
-
-
-
?
beta-thiogalactoside + O2
beta-thiogalacto-hexodialdose + H2O2
Polyporus circinatus
-
more rapidly oxidized than beta-D-galactose
-
?
ceramide dihexoside + O2
? + H2O2
-
higher activity than free substrate, very low activity as vesicle-bound substrate
-
?
ceramide trihexoside + O2
? + H2O2
-
vesicle-bound and free substrate
-
?
corn arabinoxylan + O2
?
-
-
-
?
D-Gal-beta-(1-3)-D-Gal-beta-(1-1)-L-Gro + O2
? + H2O2
-
low activity
-
?
D-Gal-beta-(1-3)-D-Gal-beta-(1-3)-D-Gal-(1-1)-L-Gro + O2
? + H2O2
-
low activity
-
?
D-Gal-beta-(1-3)-D-Gal-beta-(1-6)-D-Gal-beta-(1-1)-L-Gro + O2
? + H2O2
-
lower activity than with a reversed beta-1-6-linkage
-
?
D-Gal-beta-(1-3)-[D-Gal-beta-(1-6)]-D-Gal-beta-(1-1)-L-Gro + O2
? + H2O2
-
best oligosaccharide oxidized
-
?
D-Gal-beta-(1-6)-D-Gal-beta-(1-1)-L-Gro + O2
? + H2O2
-
faster oxidation than corresponding beta-1-3-linked components
-
?
D-Gal-beta-(1-6)-D-Gal-beta-(1-3)-D-Gal-beta-(1-1)-L-Gro + O2
? + H2O2
-
improved activity
-
?
D-galactopyranose + ferricyanide
D-galacto-hexodialdose + ferrocyanide
-
ferricyanide poorly replaces O2 as electron acceptor
-
?
D-galactosamine + O2
? + H2O2
D-galactose + O2
D-galacto-hexodialdose + H2O2
D-galactosylpyranoside + O2
D-galacto-hexodialdose + H2O2
D-glucosylpyranoside + O2
D-gluco-hexodialdose + H2O2
-
very low activity
-
?
D-raffinose + O2
? + H2O2
-
-
-
?
D-xylose + O2
? + H2O2
-
very low activity
-
?
dihydroxyacetone + O2
3-hydroxy-2-oxo-propionaldehyde + H2O2
dihydroxyacetone + O2
?
-
-
-
-
?
fetuin + O2
? + H2O2
-
bovine fetuin, native or desialylated
-
?
Forssman glycolipid + O2
? + H2O2
-
higher activity as vesicle-bound substrate, very low activity as free substrate
-
?
Gal-beta-(1-3)-[Fuc-alpha-(1-2)]-GalNAcol + O2
? + H2O2
-
no oxidation of oligosaccharides containing N-acetylgalactosamine at the non-reducing end
-
?
galactan + O2
? + H2O2
-
derived from snail, Lymnea stagnalis galactan best substrate
-
?
galactogen + O2
? + H2O2
-
substrate from Helix pomatia, galactose oxidase acts upon a specific subterminal nonreducing D-galactosyl residue
-
?
galactoglucomannan + O2
?
-
-
-
?
galactolipid + O2
? + H2O2
-
-
-
?
galactose 1-phosphate + O2
? + H2O2
Polyporus circinatus
-
very low activity
-
?
galactose-4-SO3Na + O2
? + H2O2
-
very low activity
-
?
galactose-6-SO3Na + O2
? + H2O2
-
very low activity
-
?
galactoxyloglucan + O2
?
-
-
-
?
ganglioside + O2
? + H2O2
globoside + O2
? + H2O2
-
human and porcine globoside, vesicle-bound and free substrate, best substrate tested
-
?
glyceraldehyde + O2
? + H2O2
-
70% of the activity with glycolaldehyde
-
-
?
glycerol + O2
(S)-glyceraldehyde + H2O2
glycoaldehyde + O2
glyoxal + H2O2
-
only extracellular enzyme, low activity
-
?
glycolaldehyde + O2
glyoxal + H2O2
glycolamide + O2
? + H2O2
-
-
-
?
glycoprotein + O2
? + H2O2
-
-
-
?
guar galactomannan + O2
?
-
-
-
?
guar gum + O2
? + H2O2
-
significant activity
-
?
Helix pomatia galactomannan + O2
?
-
-
-
?
hexadecyl-(ethyleneglycol)13-D-galactose + O2
hexadecyl-(ethyleneglycol)13-D-galacto-hexodialdose + H2O2
-
-
-
-
?
hexadecyl-(ethyleneglycol)20-D-galactose + O2
hexadecyl-(ethyleneglycol)20-D-galacto-hexodialdose + H2O2
-
-
-
-
?
hexadecyl-(ethyleneglycol)6-D-galactose + O2
hexadecyl-(ethyleneglycol)6-D-galacto-hexodialdose + H2O2
-
-
-
-
?
hexadecyl-(ethyleneglycol)9-D-galactose + O2
hexadecyl-(ethyleneglycol)9-D-galacto-hexodialdose + H2O2
-
-
-
-
?
isopropyl-beta-D-thiogalactosylpyranoside + O2
isopropyl-beta-D-thiogalactosyl-hexodialdose + H2O2
Polyporus circinatus
-
43% of the activity compared to D-galactose
-
?
L-glucose + O2
L-gluco-hexodialdose + H2O2
-
very low activity
-
?
lactobionic acid + O2
?
-
-
-
?
lactylamine + O2
?
-
-
-
?
larch arabinogalactan + O2
?
-
-
-
?
locust bean galactomannan + O2
?
-
-
-
?
maltose + O2
? + H2O2
-
very low activity
-
?
melibiitol + O2
? + H2O2
Polyporus circinatus
-
-
-
?
melibionic acid + O2
? + H2O2
Polyporus circinatus
-
low activity
-
?
methyl alpha-D-galactopyranoside + O2
methyl alpha-D-galacto-hexodialdo-1,5-pyranoside + H2O2
methyl alpha-D-galactopyranoside + O2
methyl alpha-D-galacto-hexodialdose + H2O2
-
-
-
-
?
methyl beta-D-mannopyranoside + O2
?
-
-
-
?
methyl beta-D-thiogalactosylpyranoside + O2
methyl beta-D-thiogalacto-hexodialdose + H2O2
Polyporus circinatus
-
-
-
?
mucin + O2
? + H2O2
-
bovine submaxillary mucin, native and desialylated
-
?
N-acetyl-D-galactosamine + O2
? + H2O2
N-acetyllactosamine + O2
?
-
-
-
?
N-glycolylneuraminic acid + O2
(2R,4S,5R,6R)-2,4-dihydroxy-5-(2-oxoacetamido)-6-[(1R,2R)-1,2,3-trihydroxypropyl]oxane-2-carboxylic acid + H2O2
N-glycolylneuraminic acid can be selectively oxidized by an engineered variant of galactose oxidase without any reaction toward Neu5Ac. Neu5Gc is also oxidized when it is part of a typical animal oligosaccharide motif and when it is attached to a protein-linked N-glycan
-
-
?
o-nitrophenyl beta-D-galactoside + O2
1-O-(o-nitrophenyl)-alpha-D-galactohexodialdose + H2O2
p-nitrophenyl alpha-D-galactoside + O2
1-O-(p-nitrophenyl)-alpha-D-galactohexodialdose + H2O2
-
more reactive than p-nitrophenyl-beta-D-galactoside
-
?
p-nitrophenyl beta-D-galactoside + O2
1-O-(p-nitrophenyl)-beta-D-galactohexodialdose + H2O2
-
less reactive than p-nitrophenyl-alpha-D-galactoside
-
?
planteose + O2
? + H2O2
Polyporus circinatus
-
-
-
?
raffinose + O2
6''-aldehydoraffinose + 6''-carboxyraffinose + H2O2 + H2O
sphingoglycolipid + O2
? + H2O2
-
-
-
?
spruce galactoglucomannan + O2
?
-
-
-
?
sucrose + O2
? + H2O2
-
very low activity
-
?
talose + O2
? + H2O2
-
-
-
-
?
tamarind galactoxyloglucan + O2
?
-
-
-
?
xyloglucan + O2
? + H2O2
-
-
-
?
additional information
?
-
1-methyl-alpha-D-galactopyranoside + O2
1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
-
-
-
-
?
1-methyl-alpha-D-galactopyranoside + O2
1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
-
-
-
-
?
1-methyl-beta-D-galactopyranoside + O2
? + H2O2
-
in the oxidation of methyl-beta-D-galactopyranoside, a dimeric product, a water elimination product, and an alpha,beta-unsaturated aldehyde occur among the mix of products. In the case of oxidized beta-galactose, the unsaturated aldehyde likely forms in the reaction
-
-
?
1-methyl-beta-D-galactopyranoside + O2
? + H2O2
in the oxidations of methyl-alpha-D-galactopyranoside and methyl-beta-D-galactopyranoside, a dimeric product, a water elimination product, and an alpha,beta-unsaturated aldehyde occur among the mix of products. In the case of oxidized beta-galactose, the unsaturated aldehyde likely forms in the reaction
-
-
?
1-methyl-beta-D-galactopyranoside + O2
? + H2O2
-
in the oxidation of methyl-beta-D-galactopyranoside, a dimeric product, a water elimination product, and an alpha,beta-unsaturated aldehyde occur among the mix of products. In the case of oxidized beta-galactose, the unsaturated aldehyde likely forms in the reaction
-
-
?
1-methyl-beta-D-galactopyranoside + O2
? + H2O2
-
in the oxidation of methyl-beta-D-galactopyranoside, a dimeric product, a water elimination product, and an alpha,beta-unsaturated aldehyde occur among the mix of products. In the case of oxidized beta-galactose, the unsaturated aldehyde likely forms in the reaction
-
-
?
1-methyl-beta-D-galactopyranoside + O2
? + H2O2
best substrate
-
-
?
1-methyl-beta-D-galactopyranoside + O2
? + H2O2
best substrate
-
-
?
1-methyl-beta-D-galactopyranoside + O2
? + H2O2
high activity
-
-
?
1-methyl-beta-D-galactopyranoside + O2
? + H2O2
high activity
-
-
?
1-methyl-beta-D-galactopyranoside + O2
? + H2O2
in the oxidation of methyl-beta-D-galactopyranoside, a dimeric product, a water elimination product, and an alpha,beta-unsaturated aldehyde occur among the mix of products. In the case of oxidized beta-galactose, the unsaturated aldehyde likely forms in the reaction
-
-
?
1-methyl-beta-D-galactopyranoside + O2
? + H2O2
-
in the oxidation of methyl-beta-D-galactopyranoside, a dimeric product, a water elimination product, and an alpha,beta-unsaturated aldehyde occur among the mix of products. In the case of oxidized beta-galactose, the unsaturated aldehyde likely forms in the reaction
-
-
?
1-methyl-beta-D-galactopyranoside + O2
? + H2O2
in the oxidation of methyl-beta-D-galactopyranoside, a dimeric product, a water elimination product, and an alpha,beta-unsaturated aldehyde occur among the mix of products. In the case of oxidized beta-galactose, the unsaturated aldehyde likely forms in the reaction
-
-
?
1-methyl-beta-D-galactopyranoside + O2
? + H2O2
in the oxidation of methyl-beta-D-galactopyranoside, a dimeric product, a water elimination product, and an alpha,beta-unsaturated aldehyde occur among the mix of products. In the case of oxidized beta-galactose, the unsaturated aldehyde likely forms in the reaction
-
-
?
1-O-methyl-alpha-D-galactosylpyranoside + O2
1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
-
-
-
?
1-O-methyl-alpha-D-galactosylpyranoside + O2
1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
-
-
-
?, ir
1-O-methyl-alpha-D-galactosylpyranoside + O2
1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
-
very fast reaction
-
?
1-O-methyl-alpha-D-galactosylpyranoside + O2
1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
-
less reactive than nitrophenyl alpha-galactosides
-
?
1-O-methyl-alpha-D-galactosylpyranoside + O2
1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
-
unusually large kinetic isotope effect for oxidation of the alpha-deuterated alcohol
-
?
1-O-methyl-alpha-D-galactosylpyranoside + O2
1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
Polyporus circinatus
-
-
-
?
1-O-methyl-alpha-D-galactosylpyranoside + O2
1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
Polyporus circinatus
-
-
-
?
1-O-methyl-alpha-D-galactosylpyranoside + O2
1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
Polyporus circinatus
-
-
-
?
1-O-methyl-alpha-D-glucosylpyranoside + O2
1-O-methyl-alpha-D-gluco-hexodialdose + H2O2
-
-
-
?
1-O-methyl-alpha-D-glucosylpyranoside + O2
1-O-methyl-alpha-D-gluco-hexodialdose + H2O2
-
very low activity
-
?
1-O-methyl-beta-D-galactosylpyranoside + O2
1-O-methyl-beta-D-galacto-hexodialdose + H2O2
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beta-configuration preferred
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?
1-O-methyl-beta-D-galactosylpyranoside + O2
1-O-methyl-beta-D-galacto-hexodialdose + H2O2
-
-
-
?
1-O-methyl-beta-D-galactosylpyranoside + O2
1-O-methyl-beta-D-galacto-hexodialdose + H2O2
-
less reactive than nitrophenyl alpha-galactosides
-
?
1-O-methyl-beta-D-galactosylpyranoside + O2
1-O-methyl-beta-D-galacto-hexodialdose + H2O2
-
48% higher activity compared to D-galactose
-
?
1-O-methyl-beta-D-galactosylpyranoside + O2
1-O-methyl-beta-D-galacto-hexodialdose + H2O2
-
transfer of one electron to O2 in a transition state which is stabilized by a hydrogen bond from the Cu2+-OH2, a rate determining electron transfer that is catalyzed by partial proton transfer
-
?
1-O-methyl-beta-D-galactosylpyranoside + O2
1-O-methyl-beta-D-galacto-hexodialdose + H2O2
-
one or more tryptophan residues, the Cu(II) atom and the sugar substrate interact within the native enzyme
-
?
1-O-methyl-beta-D-galactosylpyranoside + O2
1-O-methyl-beta-D-galacto-hexodialdose + H2O2
Polyporus circinatus
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highly active
-
1.1.3.9 galactose oxidase
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