Information on EC 1.1.3.7 - aryl-alcohol oxidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.3.7
-
RECOMMENDED NAME
GeneOntology No.
aryl-alcohol oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an aromatic primary alcohol + O2 = an aromatic aldehyde + H2O2
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
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reduction
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SYSTEMATIC NAME
IUBMB Comments
aryl-alcohol:oxygen oxidoreductase
Oxidizes many primary alcohols containing an aromatic ring; best substrates are (2-naphthyl)methanol and 3-methoxybenzyl alcohol.
CAS REGISTRY NUMBER
COMMENTARY hide
9028-77-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
; encoded by sao gene
SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
no activity in Fusarium oxysporum
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-
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Manually annotated by BRENDA team
no activity in Fusarium oxysporum BAFC 738
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Manually annotated by BRENDA team
no activity in Inocutis jamaicensis
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Manually annotated by BRENDA team
no activity in Phanerochaete chrysosporium
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-
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Manually annotated by BRENDA team
no activity in Phanerochaete chrysosporium NRRL 6361
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Manually annotated by BRENDA team
Pleurotus laciniatocrenatus
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strains BRFM 1093 and 521/FGSC 9021
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R,S)-4-methoxybenzyl alcohol + O2
1-(4-methoxyphenyl)ethanol + H2O2
show the reaction diagram
-
over 98% excess of the R enantiomer after treatment of racemic 1-(4-methoxyphenyl)ethanol, the hydride transfer is highly stereoselective
-
-
?
(S)-1-(4-fluorophenyl)ethanol + O2
1-(4-fluorophenyl)acetaldehyde + H2O2
show the reaction diagram
-
mutant F501A
-
-
?
1,1'-binaphthalene + O2
?
show the reaction diagram
1,2,3,4,5-pentachlorobenzene + O2
?
show the reaction diagram
1,2,3,4-tetrachlorobenzene + O2
?
show the reaction diagram
1,2,4,5-tetrachlorobenzene + O2
?
show the reaction diagram
1,2-binaphthalene + O2
?
show the reaction diagram
1-(2-naphthalenylmethyl)-naphthalene + O2
?
show the reaction diagram
1-amino-9,10-anthracenedione + O2
?
show the reaction diagram
1-chloro-9,10-anthracenedione + O2
?
show the reaction diagram
1-naphthalene methanol + O2
alpha-naphthaldehyde + H2O2
show the reaction diagram
-
27% of the activity with cinnamyl alcohol
-
-
?
2,4-dichloroaniline + O2
?
show the reaction diagram
2,4-dimethoxybenzyl alcohol + O2
2,4-dimethoxybenzaldehyde + H2O2
show the reaction diagram
2,4-dimethoxybenzyl alcohol + O2
2,4-dimethoxybenzyl aldehyde + H2O2
show the reaction diagram
-
50% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 75% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II
-
-
?
2,4-hexadien-1-ol + O2
2,4-hexadienal + H2O2
show the reaction diagram
2,4-hexadien-1-ol + O2
2,4-hexandienal + H2O2
show the reaction diagram
-
-
-
-
?
2,4-hexadien-1-ol + O2
?
show the reaction diagram
-
-
-
?
2,4-hexadien-1-ol + O2
? + H2O2
show the reaction diagram
2,4-hexadienal + O2
2,4-hexadienoate + H2O2
show the reaction diagram
-
-
-
-
?
2,5-diformylfuran + O2
2,5-furandicarboxylic acid + H2O2
show the reaction diagram
2,5-hydroxymethylfurancarboxylic acid + O2
2,5-furandicarboxylic acid + H2O2
show the reaction diagram
-
very low activity
-
-
?
2,6-dichloroaniline + O2
?
show the reaction diagram
2-hydroxybenzyl alcohol + O2
2-hydroxybenzaldehyde + H2O2
show the reaction diagram
2-methoxybenzyl alcohol + O2
2-methoxybenzaldehyde + H2O
show the reaction diagram
-
23% of the activity with 2-hydroxybenzyl alcohol
-
?
2-methoxybenzyl alcohol + O2
2-methoxybenzylaldehyde + H2O
show the reaction diagram
2-methylbenzyl alcohol + O2
2-methylbenzylaldehyde + H2O
show the reaction diagram
2-naphthalenemethanol + O2
2-naphthaleneformaldehyde + H2O
show the reaction diagram
-
745.7% of the activity with benzyl alcohol
-
?
2-naphthalenemethanol + O2
?
show the reaction diagram
-
-
-
-
?
2-naphthylmethanol + O2
2-naphthaldehyde + H2O2
show the reaction diagram
2-phenylethyl alcohol + O2
2-phenylacetaldehyde + H2O
show the reaction diagram
3,4-difluorobenzaldehyde + O2
3,4-difluorobenzoic acid
show the reaction diagram
-
-
-
-
?
3,4-difluorobenzaldehyde + O2
3,4-difluorobenzoic acid + H2O2
show the reaction diagram
-
-
-
-
r
3,4-dimethoxybenzyl alcohol + O2
3,4-dimethoxybenzaldehyde + H2O2
show the reaction diagram
3,4-dimethoxybenzyl alcohol + O2
veratryl aldehyde + H2O2
show the reaction diagram
3,5-dimethoxybenzyl alcohol + O2
3,5-dimethoxy benzaldehyde + H2O2
show the reaction diagram
-
7% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 8% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II
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-
?
3-anisyl alcohol + O2
3-anisyl aldehyde + H2O2
show the reaction diagram
3-chloro-4-anisaldehyde + O2
3-chloro-4-anisic acid + H2O2
show the reaction diagram
-
-
-
-
r
3-chloro-4-anisyl alcohol + O2
3-chloro-4-anisaldehyde + H2O2
show the reaction diagram
-
-
-
-
?
3-chloro-4-anisyl alcohol + O2
3-chloro-4-anisyl aldehyde + H2O2
show the reaction diagram
-
high activity
-
-
?
3-chloro-4-methoxybenzyl alcohol + O2
3-chloro-4-methoxybenzaldehyde + H2O2
show the reaction diagram
-
ternary mechanism
-
-
?
3-chlorobenzaldehyde + O2
3-chlorobenzoic acid + H2O2
show the reaction diagram
-
-
-
-
r
3-chlorobenzyl alcohol + O2
3-chlorobenzaldehyde + H2O2
show the reaction diagram
3-chlorobenzyl alcohol + O2
3-chlorobenzyl aldehyde + H2O2
show the reaction diagram
-
-
-
-
?
3-fluorobenzaldehyde + O2
3-fluorobenzoic acid + H2O2
show the reaction diagram
-
-
-
-
r
3-fluorobenzyl alcohol + O2
3-fluorobenzaldehyde + H2O2
show the reaction diagram
3-fluorobenzyl alcohol + O2
3-fluorobenzyl aldehyde + H2O2
show the reaction diagram
-
low activity
-
-
?
3-fluorobenzyl alcohol + O2
?
show the reaction diagram
-
-
-
?
3-hydroxy-4-methoxybenzyl alcohol + O2
3-hydroxy-4-methoxybenzaldehyde + H2O2
show the reaction diagram
-
62% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 71% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II
-
-
?
3-hydroxybenzyl alcohol + O2
3-hydroxybenzaldehyde + H2O2
show the reaction diagram
3-methoxybenzyl alcohol + O2
3-methoxybenzaldehyde + H2O2
show the reaction diagram
3-methoxybenzyl alcohol + O2
3-methoxybenzylaldehyde + H2O
show the reaction diagram
recombinant enzyme shows 1% of the activity with 2-hydroxybenzyl alcohol
-
-
?
3-phenoxybenzyl alcohol + O2
3-phenoxybenzaldehyde + H2O2
show the reaction diagram
-
35% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 18% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II
-
-
?
4-anisaldehyde + O2
4-anisic acid + H2O2
show the reaction diagram
-
-
-
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r
4-anisyl alcohol + O2
4-anisaldehyde + H2O2
show the reaction diagram
4-anisyl alcohol + O2
4-anisyl aldehyde + H2O2
show the reaction diagram
4-chlorobenzaldehyde + O2
4-chlorobenzoic acid + H2O2
show the reaction diagram
-
-
-
-
r
4-chlorobenzyl alcohol + O2
4-chlorobenzaldehyde + H2O2
show the reaction diagram
-
-
-
-
?
4-chlorobenzyl alcohol + O2
4-chlorobenzyl aldehyde + H2O2
show the reaction diagram
-
-
-
-
?
4-fluorobenzaldehyde + O2
4-fluorobenzoic acid + H2O2
show the reaction diagram
-
-
-
-
r
4-fluorobenzyl alcohol + O2
4-fluorobenzaldehyde + H2O2
show the reaction diagram
-
-
-
-
?
4-fluorobenzyl alcohol + O2
4-fluorobenzyl aldehyde + H2O2
show the reaction diagram
-
-
-
-
?
4-hydroxy-3-methoxybenzyl alcohol + O2
4-hydroxy-3-methoxybenzaldehyde + H2O2
show the reaction diagram
4-hydroxybenzyl alcohol + O2
4-hydroxybenzaldehyde + H2O2
show the reaction diagram
4-hydroxybenzyl alcohol + O2
4-hydroxybenzyl aldehyde + H2O2
show the reaction diagram
-
7.6% of the activity with anisyl alcohol
-
-
?
4-methoxybenzyl alcohol + O2
4-methoxybenzaldehyde + H2O2
show the reaction diagram
4-methoxycinnamyl alcohol + O2
4-methoxycinnamaldehyde + H2O2
show the reaction diagram
-
-
-
-
r
4-nitrobenzaldehyde + O2
4-nitrobenzoic acid
show the reaction diagram
-
-
-
-
?
4-nitrobenzaldehyde + O2
4-nitrobenzoic acid + H2O2
show the reaction diagram
-
-
-
-
r
4-nitrobenzyl alcohol + O2
4-nitrobenzaldehyde + H2O2
show the reaction diagram
-
-
-
-
r
5-hydroxymethylfurfural + O2
2,5-diformylfuran + H2O2
show the reaction diagram
5-hydroxymethylfurfural + O2
2,5-hydroxymethylfurancarboxylic acid + H2O2
show the reaction diagram
-
-
-
-
?
7H-benz[DE]anthracen-7-one + O2
?
show the reaction diagram
9,10-anthracenedione + O2
?
show the reaction diagram
anisyl alcohol + O2
anisaldehyde + H2O2
show the reaction diagram
anisyl alcohol + O2
anisyl aldehyde + H2O2
show the reaction diagram
-
-
-
-
?
benzaldehyde + O2
benzoic acid + H2O2
show the reaction diagram
-
-
-
-
r
benzyl alcohol + O2
benzaldehyde + H2O2
show the reaction diagram
beta-naphthylcarbinol + O2
beta-naphthaldehyde + H2O2
show the reaction diagram
cinnamyl alcohol + O2
cinnamaldehyde + H2O2
show the reaction diagram
cinnamyl alcohol + O2
cinnamic aldehyde + H2O2
show the reaction diagram
cinnamyl alcohol + O2
cinnamyl aldehyde + H2O2
show the reaction diagram
-
-
-
-
?
coniferyl alcohol + O2
coniferyl aldehyde + H2O2
show the reaction diagram
-
13% of the activity with cinnamyl alcohol
-
-
?
cyclohexyl alcohol + O2
cyclohexyl aldehyde + H2O2
show the reaction diagram
-
12% of the activity with cinnamyl alcohol
-
-
?
diphenylether + O2
?
show the reaction diagram
diphenylsulfone + O2
?
show the reaction diagram
Direct Red 5B + O2
3-diazenyl-7-[(phenylcarbonyl)amino]naphthalene-2-sulfonic acid + H2O2
show the reaction diagram
-
degradation, dye decolorizing
product identification by GC-MS analysis
-
?
ethanol + O2
acetaldehyde + H2O2
show the reaction diagram
isovanillyl alcohol + O2
isovanillyl aldehyde + H2O2
show the reaction diagram
-
-
-
-
?
m-anisyl alcohol + O2
m-anisaldehyde + H2O2
show the reaction diagram
N-phenyl-1-naphthalenamine + O2
?
show the reaction diagram
n-propanol + O2
propionaldehyde + H2O2
show the reaction diagram
naphthalene + O2
?
show the reaction diagram
veratraldehyde + O2
veratric acid + H2O2
show the reaction diagram
-
-
-
-
r
veratryl alcohol + 2,6-dichlorophenol indophenol
veratrylaldehyde + red. 2,6-dichlorophenol indophenol
show the reaction diagram
-
-
-
-
?
veratryl alcohol + O2
? + H2O2
show the reaction diagram
-
-
-
-
?
veratryl alcohol + O2
veratraldehyde + H2O2
show the reaction diagram
veratryl alcohol + O2
veratryl aldehyde + H2O2
show the reaction diagram
veratryl alcohol + O2
veratrylaldehyde + H2O2
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(R,S)-4-methoxybenzyl alcohol + O2
1-(4-methoxyphenyl)ethanol + H2O2
show the reaction diagram
-
over 98% excess of the R enantiomer after treatment of racemic 1-(4-methoxyphenyl)ethanol, the hydride transfer is highly stereoselective
-
-
?
1,1'-binaphthalene + O2
?
show the reaction diagram
-
degradation, partial removal from soil
-
-
?
1,2,3,4,5-pentachlorobenzene + O2
?
show the reaction diagram
-
degradation, partial removal from soil
-
-
?
1,2,3,4-tetrachlorobenzene + O2
?
show the reaction diagram
-
degradation, complete removal from soil
-
-
?
1,2,4,5-tetrachlorobenzene + O2
?
show the reaction diagram
-
degradation, complete removal from soil
-
-
?
1,2-binaphthalene + O2
?
show the reaction diagram
-
degradation, partial removal from soil
-
-
?
1-(2-naphthalenylmethyl)-naphthalene + O2
?
show the reaction diagram
-
degradation, partial removal from soil
-
-
?
1-amino-9,10-anthracenedione + O2
?
show the reaction diagram
-
degradation, partial removal from soil
-
-
?
1-chloro-9,10-anthracenedione + O2
?
show the reaction diagram
-
degradation, partial removal from soil
-
-
?
2,4-dichloroaniline + O2
?
show the reaction diagram
-
degradation, complete removal from soil
-
-
?
2,4-dimethoxybenzyl alcohol + O2
2,4-dimethoxybenzaldehyde + H2O2
show the reaction diagram
-
-
-
-
r
2,4-hexadien-1-ol + O2
?
show the reaction diagram
O94219
-
-
-
?
2,5-diformylfuran + O2
2,5-furandicarboxylic acid + H2O2
show the reaction diagram
2,6-dichloroaniline + O2
?
show the reaction diagram
-
degradation, complete removal from soil
-
-
?
2-hydroxybenzyl alcohol + O2
2-hydroxybenzaldehyde + H2O2
show the reaction diagram
2-naphthylmethanol + O2
2-naphthaldehyde + H2O2
show the reaction diagram
-
best substrate
-
-
r
3-methoxybenzyl alcohol + O2
3-methoxybenzaldehyde + H2O2
show the reaction diagram
-
-
-
-
r
4-anisyl alcohol + O2
4-anisaldehyde + H2O2
show the reaction diagram
O94219
the substrate is an extracellular fungal metabolite
-
-
?
4-anisyl alcohol + O2
4-anisyl aldehyde + H2O2
show the reaction diagram
-
preferred substrate
-
-
?
4-hydroxy-3-methoxybenzyl alcohol + O2
4-hydroxy-3-methoxybenzaldehyde + H2O2
show the reaction diagram
-
-
-
-
r
4-hydroxybenzyl alcohol + O2
4-hydroxybenzaldehyde + H2O2
show the reaction diagram
-
-
-
-
r
4-methoxybenzyl alcohol + O2
4-methoxybenzaldehyde + H2O2
show the reaction diagram
4-methoxycinnamyl alcohol + O2
4-methoxycinnamaldehyde + H2O2
show the reaction diagram
-
-
-
-
r
4-nitrobenzyl alcohol + O2
4-nitrobenzaldehyde + H2O2
show the reaction diagram
-
-
-
-
r
5-hydroxymethylfurfural + O2
2,5-diformylfuran + H2O2
show the reaction diagram
7H-benz[DE]anthracen-7-one + O2
?
show the reaction diagram
-
degradation, partial removal from soil
-
-
?
9,10-anthracenedione + O2
?
show the reaction diagram
-
degradation, partial removal from soil
-
-
?
benzyl alcohol + O2
benzaldehyde + H2O2
show the reaction diagram
cinnamyl alcohol + O2
cinnamaldehyde + H2O2
show the reaction diagram
-
high activity
-
-
r
diphenylether + O2
?
show the reaction diagram
-
degradation, complete removal from soil
-
-
?
diphenylsulfone + O2
?
show the reaction diagram
-
degradation, partial removal from soil
-
-
?
N-phenyl-1-naphthalenamine + O2
?
show the reaction diagram
-
degradation, complete removal from soil
-
-
?
n-propanol + O2
propionaldehyde + H2O2
show the reaction diagram
naphthalene + O2
?
show the reaction diagram
-
degradation, complete removal from soil
-
-
?
veratryl alcohol + O2
veratraldehyde + H2O2
show the reaction diagram
veratryl alcohol + O2
veratryl aldehyde + H2O2
show the reaction diagram
Pleurotus laciniatocrenatus
-
-
-
-
?
veratryl alcohol + O2
veratrylaldehyde + H2O2
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
flavin
-
prosthetic group
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cu2+
-
required for catalysis
additional information
Pleurotus laciniatocrenatus
-
no activation or induction by supplementation of CuSO4
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-hydroxybenzyl alcohol
3-Phenyl-1-propanol
4-anisic acid
-
competitive
4-Hydroxybenzyl alcohol
-
shows substrate inhibition at higher concentration
4-methoxybenzylamine
-
uncompetitive, pH-dependent inhibition, best at pH 8.0
Ag+
-
0.5 mM, 38% inhibition
anisyl alcohol
-
shows substrate inhibition at higher concentration
benzylmethyl ether
-
competitive
CaCl2
-
15% inhibition at 0.5 mM
chavicol
-
competitive
cinnamyl alcohol
-
shows substrate inhibition at higher concentration
dithiothreitol
-
7% inhibition at 0.5 mM
EDTA
-
24% inhibition at 0.5 mM
HgCl2
-
1 mM, 92% inhibition
L-cysteine
-
20% inhibition at 0.5 mM
MgCl2
-
16% inhibition at 0.5 mM
MnCl2
-
14% inhibition at 0.5 mM
NaN3
-
10 mM, 19% inhibition
p-methoxybenzyl alcohol
-
substrate inhibition at high concentrations
Pb2+
-
0.5 mM, 63% inhibition
PCMB
-
1 mM, 95% inhibition
phenol
-
competitive
Sodium azide
-
mixed competitive inhibition, 90% inhibition at 0.5 mM
Toluene
-
competitive
ZnSO4
-
8% inhibition at 0.5 mM
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
Pleurotus laciniatocrenatus
-
enzyme is induced by glucose depletion, no activation or induction by supplementation of vanillic acid
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.79 - 1.82
2,4-Dimethoxybenzyl alcohol
0.015 - 0.263
2,4-hexadien-1-ol
13
2,4-hexadienal
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
3.3
2,5-diformylfuran
-
pH 6.0, 25°C
-
28 - 132
2-hydroxybenzyl alcohol
3
3,4-difluorobenzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.39 - 2.88
3,4-dimethoxybenzyl alcohol
0.211 - 0.734
3-anisyl alcohol
0.7
3-chloro-4-anisaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.014
3-chloro-4-anisyl alcohol
-
pH 6.0, 24°C
1.5
3-Chlorobenzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.107
3-Chlorobenzyl alcohol
-
pH 6.0, 24°C
2.2
3-Fluorobenzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.554
3-fluorobenzyl alcohol
-
pH 6.0, 24°C
0.22 - 4.91
3-Methoxybenzyl alcohol
0.7 - 0.8
4-anisaldehyde
0.015 - 0.053
4-anisyl alcohol
4.7
4-Chlorobenzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.132
4-Chlorobenzyl alcohol
-
pH 6.0, 24°C
4.9
4-Fluorobenzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.553
4-fluorobenzyl alcohol
-
pH 6.0, 24°C
0.042
4-Hydroxybenzyl alcohol
-
pH 6.0, 30°C
0.017 - 3.82
4-methoxybenzyl alcohol
2 - 5
4-Nitrobenzaldehyde
1.6
5-hydroxymethylfurfural
-
pH 6.0, 25°C
0.0048 - 0.836
anisyl alcohol
7
benzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.03 - 10.8
benzyl alcohol
0.035 - 0.708
cinnamyl alcohol
0.831
isovanillyl alcohol
-
pH 6.0, 24°C
0.22 - 0.3
m-anisyl alcohol
0.017 - 0.348
O2
0.03 - 0.04
p-anisyl alcohol
0.025
Vanillyl alcohol
-
-
8
veratraldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.027 - 2
veratryl alcohol
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
30
2,4-Dimethoxybenzyl alcohol
-
pH 6.0, 30°C
1 - 206
2,4-hexadien-1-ol
0.33
2,4-hexadienal
-
wild type enzyme, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
31.4
2,5-diformylfuran
-
pH 6.0, 25°C
-
0.867
3,4-difluorobenzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
30 - 105.7
3,4-dimethoxybenzyl alcohol
1 - 26
3-anisyl alcohol
0.057
3-chloro-4-anisaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
46
3-chloro-4-anisyl alcohol
-
pH 6.0, 24°C
0.85
3-Chlorobenzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
22
3-Chlorobenzyl alcohol
-
pH 6.0, 24°C
0.883
3-Fluorobenzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
6
3-fluorobenzyl alcohol
-
pH 6.0, 24°C
115.4
3-Methoxybenzyl alcohol
recombinant enzyme, at pH 6.0 and 30°C
0.012 - 0.05
4-anisaldehyde
3 - 142
4-anisyl alcohol
1.05
4-Chlorobenzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
51
4-Chlorobenzyl alcohol
-
pH 6.0, 24°C
0.367
4-Fluorobenzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
32
4-fluorobenzyl alcohol
-
pH 6.0, 24°C
75
4-Hydroxybenzyl alcohol
-
pH 6.0, 30°C
0.069 - 208
4-methoxybenzyl alcohol
1.21 - 1.633
4-Nitrobenzaldehyde
20.1
5-hydroxymethylfurfural
-
pH 6.0, 25°C
45 - 203
anisyl alcohol
0.5
benzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
1 - 38
benzyl alcohol
65 - 88
cinnamyl alcohol
127
isovanillyl alcohol
-
pH 6.0, 24°C
8.27
m-anisyl alcohol
-
-
48.5
p-anisyl alcohol
-
-
0.13
veratraldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
2 - 116
veratryl alcohol
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
16.5
2,4-Dimethoxybenzyl alcohol
-
pH 6.0, 30°C
866 - 4270
2,4-hexadien-1-ol
0.025
2,4-hexadienal
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.157
2,5-diformylfuran
-
pH 6.0, 25°C
-
0.0167
2,5-hydroxymethylfurancarboxylic acid
-
pH 6.0, 25°C
-
0.282
3,4-difluorobenzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
39.7
3,4-dimethoxybenzyl alcohol
recombinant enzyme, at pH 6.0 and 30°C
0.085
3-chloro-4-anisaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.643
3-Chlorobenzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.407
3-Fluorobenzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
23.5
3-Methoxybenzyl alcohol
recombinant enzyme, at pH 6.0 and 30°C
0.013 - 0.087
4-anisaldehyde
0.223
4-Chlorobenzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.075
4-Fluorobenzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
1790
4-Hydroxybenzyl alcohol
-
pH 6.0, 30°C
0.019 - 5160
4-methoxybenzyl alcohol
0.315 - 0.597
4-Nitrobenzaldehyde
0.215
5-hydroxymethylfurfural
-
pH 6.0, 25°C
3466 - 9380
anisyl alcohol
0.073
benzaldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
0.2 - 131
benzyl alcohol
2510
cinnamyl alcohol
-
pH 6.0, 30°C
0.0167
veratraldehyde
-
wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0
28 - 440
veratryl alcohol
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.48
3-Phenyl-1-propanol
0.08
4-anisic acid
0.25
4-methoxybenzylamine
-
pH 8.0, 24°C
1.35
benzylmethyl ether
-
pH 6.0, 24°C
0.11
chavicol
-
pH 6.0, 24°C
1.92
phenol
0.75
Toluene
-
pH 6.0, 24°C
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.155
-
crude enzyme, using veratryl alcohol as substrate, at pH 6.0, 40°C
0.66
-
soil-extracted extracellular enzyme
1.58
-
after 10.19fold purification, using veratryl alcohol as substrate, at pH 6.0, 40°C
21.75
-
purified native enzyme, pH 3.0, 40°C
24
-
purified recombinant H91N FX7 mutant, pH 6.0, 24°C
41.5
-
pH 6.0, 30°C, isozyme III
60.2
-
pH 6.0, 30°C, isozyme I
63.6
-
pH 6.0, 30°C, isozyme II
74
-
purified recombinant wild-type enzyme, pH 6.0, 24°C
88.1
purified recombinant enzyme, pH 6.0, 30°C
96
purified recombinant enzyme from Escherichia coli
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 7
the enzyme shows more than 90% of maximal activity, measured at pH 5.0, in the pH range between pH 4 and pH 6 and more than 80% between pH 3 and pH 7
3.5 - 8
-
pH 3.5: about 70% of maximal activity, pH 8.0: about 65% of maximal activity
4 - 9
-
AAO shows no pH dependence of kcat or catalytic efficiency for the substrates analyzed, AAO is unstable above pH 9.0