We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments Contains FAD. The enzyme, characterized from the millipede Chamberlinius hualienensis, is segregated from its substrate, which is contained in special sacs. The sacs are ruptured during defensive behavior, allowing the enzyme and substrate to mix in special reaction chambers leading to production of the defensive chemical benzoyl cyanide.
The expected taxonomic range for this enzyme is: Chamberlinius hualienensis
Synonyms
ChuaMOX ,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ChuaMOX
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(R)-mandelonitrile + O2 = benzoyl cyanide + H2O2
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(R)-mandelonitrile:oxygen oxidoreductase
Contains FAD. The enzyme, characterized from the millipede Chamberlinius hualienensis, is segregated from its substrate, which is contained in special sacs. The sacs are ruptured during defensive behavior, allowing the enzyme and substrate to mix in special reaction chambers leading to production of the defensive chemical benzoyl cyanide.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(3E,5E)-2-hydroxydeca-3,5-dienenitrile + O2
(3E,5E)-2-oxodeca-3,5-dienenitrile + H2O2
10% of the activity with (R)-mandelonitrile
-
-
?
(E)-2-hydroxy-4-phenylbut-3-enenitrile + O2
(3E)-2-oxo-4-phenylbut-3-enenitrile + H2O2
29% of the activity with (R)-mandelonitrile
-
-
?
(R)-mandelonitrile + O2
benzoyl cyanide + H2O2
-
enzyme stoichiometrically catalyzes oxygen consumption and synthesis of benzoyl cyanide and hydrogen peroxide from mandelonitrile
-
?
(R,S)-mandelonitrile + O2
benzoyl cyanide + H2O2
-
-
-
?
2-(3-bromophenyl)-2-hydroxyacetonitrile + O2
(3-bromophenyl)(oxo)acetonitrile + H2O2
22% of the activity with (R)-mandelonitrile
-
-
?
2-hydroxy-2-(2-methoxyphenyl)acetonitrile + O2
(2-methoxyphenyl)(oxo)acetonitrile + H2O2
17% of the activity with (R)-mandelonitrile
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
FAD
sequence contains a FAD binding motif in the T22-S62 region
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ferric chloride
1 mM, 124% of initial activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
8-hydroxyquinoline
1 mM, 56% residual activity
potassium cyanide
1 mM, 12.7% residual activity
Sodium azide
1 mM, 28.6% residual activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2.1
(R,S)-mandelonitrile
pH 5.0, 25°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3.5
80% of maximum activity
5
80% of maximum activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
30
90% of maximum activity
40
90% of maximum activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
physiological function
the enzyme stoichiometrically catalyzes oxygen consumption and synthesis of benzoyl cyanide and hydrogen peroxide from mandelonitrile. The enzymatic activity is suppressed at a blood pH of 7, and the enzyme is segregated by membranes of defensive sacs from its substrate mandelonitrile which has a pH of 4.6, the optimum pH for ChuaMOX activity. Strong body muscle contractions are necessary for de novo synthesis of benzoyl cyanide
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
A0A173MZJ8_9MYRI
593
0
64605
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
monomer
1 * 67000, SDS-PAGE, 1 * 62818, calculated
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
glycoprotein
sequence contains six predicted N-glycosylation sites at resiudes N57, N79, N94, N149, N406, and N491
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
8
25-50 °C, 1 h, 80% residual activity
739691
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
70
1 h, complete loss of activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ishida, Y; Kuwahara, Y.; Dadashipour, M; Ina, A; Yamaguchi, T; Morita, M.; Ichiki, Y; Asano, Y
A sacrificial millipede altruistically protects its swarm using a drone blood enzyme, mandelonitrile oxidase
Sci. Rep.
6
26998
2016
Chamberlinius hualienensis (A0A173MZJ8), Chamberlinius hualienensis
brenda
Select items on the left to see more content.
html completed
BRENDA home
History
Enzyme Nomenclature
1.1.3.49 (R)-mandelonitrile oxidase
top
