Information on EC 1.1.3.23 - thiamine oxidase

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EC Tree 1 Oxidoreductases 1.1 Acting on the CH-OH group of donors 1.1.3 With oxygen as acceptor 1.1.3.23 thiamine oxidase

IUBMB Comments

A flavoprotein (FAD). The product differs from thiamine in replacement of -CH₂.CH₂.OH by -CH₂.COOH; the two-step oxidation proceeds without the release of the intermediate aldehyde from the enzyme.

The enzyme appears in viruses and cellular organisms

1.1.3.23
flavin
beta-cyclopiazonate
flavoprotein
8alpha
pka
imidazole
paramagnetic
oxidocyclase

Reaction Schemes

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Synonyms
thiamin dehydrogenase, thiamine dehydrogenase, thiamine oxidase, more

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SYNONYM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

thiamin dehydrogenase

thiamin oxidase

thiamin:oxygen 5-oxidoreductase

thiamine dehydrogenase

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REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

thiamine + 2 O2 + H2O = thiamine acetic acid + 2 H2O2

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REACTION TYPE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

oxidation

redox reaction

reduction

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Go to Pathway Search

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PATHWAY SOURCE

PATHWAYS

KEGG

Thiamine metabolism

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SYSTEMATIC NAME

IUBMB Comments

thiamine:oxygen 5-oxidoreductase

A flavoprotein (FAD). The product differs from thiamine in replacement of -CH2.CH2.OH by -CH2.COOH; the two-step oxidation proceeds without the release of the intermediate aldehyde from the enzyme.

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CAS REGISTRY NUMBER

COMMENTARY

96779-44-1

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SUBSTRATE

PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

Reversibility
r=reversible
ir=irreversible
?=not specified

oxythiamine + O2

? + H2O2

unidentified soil bacterium

389741, 389742, 389743

Substrates: -
Products: -

pyrithiamine + O2

? + H2O2

unidentified soil bacterium

389741, 389742, 389743

Substrates: -
Products: -

thiamine + O2

unidentified soil bacterium

389741, 389742, 389743

Substrates: -
Products: -

thiamine + O2

thiamine acetate + H2O2

2 entries

thiamine acetic acid + H2O2

thiamine + O2 + H2O

unidentified soil bacterium

389743

Substrates: -
Products: -

thiamine + O2

thiamine acetate + H2O2

unidentified soil bacterium

389741, 389742

Substrates: -
Products: -

thiamine + O2

thiamine acetate + H2O2

unidentified soil bacterium

389743

Substrates: reaction via an aldehyde intermediate which is not released
Products: -

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

thiamine + O2

unidentified soil bacterium

389741, 389742, 389743

Substrates: -
Products: -

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COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

FAD

3 entries

FAD

unidentified soil bacterium

389741

FAD

unidentified soil bacterium

8-alpha-N(1)-histidyl-FAD

389742, 389743

FAD

unidentified soil bacterium

1 mol/mol enzyme

389743

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

Cu2+

unidentified soil bacterium

389741

Hg2+

unidentified soil bacterium

389741

p-Chloromeruribenzoate

unidentified soil bacterium

389741

pyrithiamine

unidentified soil bacterium

389743

Quinacrine

unidentified soil bacterium

389741

Semicarbazide

unidentified soil bacterium

389741

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

1.72

thiamine

unidentified soil bacterium

389741

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

H2O2

unidentified soil bacterium

release of H+

389743

1.67

thiamine

unidentified soil bacterium

thiamine acetate formed

389741

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

2.18

unidentified soil bacterium

389741

additional information

2 entries

additional information

unidentified soil bacterium

389741

additional information

unidentified soil bacterium

389742

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pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

8 - 9

unidentified soil bacterium

389741

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only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks include all textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks + FRENDAco-occurrence of enzyme names and organism names (less precise)

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

unidentified soil bacterium

ATCC 25589

389741, 389742, 389743

brenda

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Highest Expressing Human Cell Lines

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Cell Line Links

Gene Links

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

41500

unidentified soil bacterium

gel filtration

389741

52000

unidentified soil bacterium

gel filtration

389742

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SUBUNIT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

monomer

unidentified soil bacterium

1 * 49000-50000, SDS-PAGE

389742

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

unidentified soil bacterium

stable for 30 min

389741

unidentified soil bacterium

not stable above

389741

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-70°C, 3 months

unidentified soil bacterium

389741

4°C, 1 week

unidentified soil bacterium

389741

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

unidentified soil bacterium

389741, 389742

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

389741

Neal, R.A.

Bacterial metabolism of thiamine. 3. Metabolism of thiamine to 3-(2-methyl-4-amino-5-pyrimidylmethyl)-4-methyl-thiazole-5-acetic acid (thiamine acetic acid) by a flavoprotein isolated from a soil microorganism

J. Biol. Chem.

245

2599-2604

1970

unidentified soil bacterium

4987737

brenda

389742

Gomez-Moreno, C.; Choy, M.; Edmondson, D.E.

Purification and properties of the bacterial flavoprotein: thiamin dehydrogenase

J. Biol. Chem.

254

7630-7635

1979

unidentified soil bacterium

468775

brenda

389743

Gomez-Moreno, C.; Edmondson, D.E.

Evidence for an aldehyde intermediate in the catalytic mechanism of thiamine oxidase

Arch. Biochem. Biophys.

239

46-52

1985

unidentified soil bacterium

2988447

brenda

Entries 1 - 20 of 3

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EXTERNAL LINKS (specific for EC-Number 1.1.3.23)

ExplorEnz (official portal for IUBMB Enzyme Nomenclature)

Expasy Enzyme Nomenclature Database

KEGG

SABIO-RK

NCBI: PubMed, Protein, Nucleotide, Structure, Gene, OMIM

Enzyme Nomenclature (alternative site)

UniProt

PDB

PROSITE Database of protein families and domains

InterPro (database of protein families, domains and functional sites)

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