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1,5-anhydro-D-fructose + O2
1,5-anhydro-3-keto-D-fructose + H2O2
-
-
yield of 1,5-anhydro-3-keto-D-fructose: 33%
?
1,5-anhydro-D-glucitol + O2
1,5-anhydro-D-fructose + H2O2
Phanerochaete gigantea
-
8% relative activity to D-glucose
yield of 1,5-anhydro-D-fructose: 100%
?
1,5-anhydro-D-glucitol + O2
?
-
18.4% of the activity with D-glucose
-
-
?
1,5-anhydro-D-sorbitol + O2
?
-
-
-
-
?
1,6-anhydro-D-glucose + O2
?
-
-
-
-
?
1-beta-aurothioglucose + O2
?
-
91% relative activity to D-glucose
-
-
?
2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) + O2
? + H2O2
-
-
-
?
2,6-dichloroindophenol + O2
? + H2O2
-
-
-
?
2,6-dimethyl-1,4-benzoquinone + O2
? + H2O2
2-chloro-1,4-benzoquinone + O2
? + H2O2
-
-
-
?
2-deoxy-2-fluoro-D-glucose + O2
2-deoxy-3-dehydro-D-glucose + H2O2
slow substrate
-
-
?
2-deoxy-D-galactose + O2
?
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
2-deoxy-D-glucose + O2
2-deoxy-2-dehydro-D-glucose + H2O2
-
-
-
r
2-deoxy-D-glucose + O2
2-deoxy-3-keto-D-glucose + H2O2
2-deoxy-D-glucose + O2
?
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
2-keto-D-glucose + O2
2,3-diketo-D-glucose + H2O2
2-methoxy-1,4-benzoquinone + O2
? + H2O2
-
-
-
?
3-deoxy-3-fluoro-beta-D-glucose + H2O2
?
3-deoxy-D-glucose + O2
2-keto-3-deoxy-D-glucose + H2O2
3-O-methyl-D-glucose + O2
2-keto-3-O-methyl-D-glucose + H2O2
-
8.6% relative activity to D-glucose
-
?
5-thioglucose + O2
2-keto-5-thioglucose + H2O2
6-deoxy-D-glucose + O2
2-keto-6-deoxy-D-glucose + H2O2
allose + O2
?
-
38% relative activity to D-glucose
-
-
?
alpha-D-glucose + O2
2-dehydro-D-glucose + H2O2
alpha-D-melibiose + O2
? + H2O2
beta-D-galactose + O2
2-dehydro-D-galactose + H2O2
poor substrate
-
-
?
beta-D-glucose + O2
beta-2-dehydro-D-glucose + H2O2
cellobiose + O2
2-dehydrocellobiose + H2O2
-
17.8% activity compared to D-glucose
-
-
?
D-arabinose + O2
?
-
1.87% of the activity with D-glucose
-
-
?
D-fructose + O2
2-dehydro-D-fructose + H2O2
D-fucono-1,5-lactone + O2
2-keto-D-gluconate + D-araboascorbate + H2O2
D-galactose + 1,4-benzoquinone
2-dehydro-D-galactose + 1,4-hydroquinone
D-galactose + 1,4-benzoquinone
2-dehydro-D-galactose + hydroquinone
-
-
-
?
D-galactose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical
2-dehydro-D-galactose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
-
-
?
D-galactose + ferricenium hexafluorophosphate
2-dehydro-D-galactose + ferrocenium hexafluorophosphate
-
-
-
-
r
D-galactose + ferricenium ion
2-dehydro-D-galactose + ferrocenium ion
-
-
-
r
D-galactose + ferrocenium hexafluorophosphate
2-dehydro-D-galactose + ferrocene
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
D-glucono-1,5-lactone + 3-methyl-2-benzothiazolinone hydrazone
2-dehydro-D-glucono-1,5-lactone
D-glucono-1,5-lactone + O2
2-dehydro-D-glucono-1,5-lactone + H2O2
-
107% activity compared to D-glucose
-
-
?
D-glucono-1,5-lactone + O2
?
D-gluconolactone + O2
? + H2O2
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + 1,4-hydroquinone
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + hydroquinone
1,4-benzoquinone is a physiologically relevant alternative electron acceptor in the oxidative half-reaction
-
-
?
D-glucose + 1,4-benzoquinone
?
D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
2-dehydro-D-glucose + ?
-
-
-
-
?
D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical
2-dehydro-D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
D-glucose + 2,6-dichloroindophenol
2-dehydro-D-glucose + ?
-
-
-
?
D-glucose + 2,6-dichloroindophenol
?
-
-
-
-
?
D-glucose + 2,6-dichlorophenolindophenol
2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
-
-
-
r
D-glucose + 2,6-dichlorophenolindophenol
3-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-glucose + 2,6-dimethyl-1,4-benzoquinone
?
-
-
-
-
?
D-glucose + 2-chloro-1,4-benzoquinone
?
-
-
-
-
?
D-glucose + 2-methoxy-1,4-benzoquinone
?
-
-
-
-
?
D-glucose + 2-methyl-1,4-benzoquinone
2-dehydro-D-glucose + 2-methylhydroquinone
-
-
-
r
D-glucose + 2-methyl-1,4-benzoquinone
?
-
-
-
-
?
D-glucose + 3-methyl-2-benzothiazolinone hydrazone
2-dehydro-D-glucose + ?
D-glucose + ferricenium hexafluorophosphate
2-dehydro-D-glucose + ferrocenium hexafluorophosphate
-
-
-
-
r
D-glucose + ferricenium hexafluorophosphate
?
D-glucose + ferricenium ion
2-dehydro-D-glucose + ferrocenium ion
D-glucose + ferricyanide
2-dehydro-D-glucose + ferrocyanide
-
-
-
r
D-glucose + ferrocenium hexafluorophosphate
2-dehydro-D-glucose + ferrocene
-
-
-
?
D-glucose + ferrocenium ion
2-dehydro-D-glucose + ?
-
-
-
?
D-glucose + methyl-1,4-benzoquinone
2-dehydro-D-glucose + methylhydroquinone
-
-
-
-
r
D-glucose + O2
2-dehydro-D-glucose + H2O2
D-glucose + O2
D-arabino-2-hexosulose + H2O2
-
the enzyme is produced during glucose starvation
-
?
D-glucose + O2
D-arabino-hexos-2-ulose + H2O2
D-glucose + O2
D-glucosone + H2O2
D-glucose + tetrabromo-1,4-benzoquinone
2-dehydro-D-glucose + tetrabromohydroquinone
-
-
-
-
r
D-glucose + tetrafluoro-1,4-benzoquinone
?
-
-
-
-
?
D-maltoheptaose + O2
?
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
D-maltopentaose + O2
?
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
D-maltose + O2
?
-
15.1% of the activity with D-glucose
-
-
?
D-maltotriose + O2
?
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
D-mannoheptose + O2
?
-
8.2% relative activity to D-glucose
-
-
?
D-mannose + O2
2-dehydro-D-mannose + H2O2
-
18.4% activity compared to D-glucose
-
-
?
D-mannose + O2
2-keto-D-mannose + H2O2
-
0.9% relative activity to D-glucose
-
?
D-mannose + O2
?
-
6.02% of the activity with D-glucose
-
-
?
D-ribose + 1,4-benzoquinone
2-dehydro-D-ribose + 1,4-hydroquinone
-
-
-
-
?
D-ribose + O2
2-dehydro-D-ribose + H2O2
-
-
-
-
?
D-trehalose + O2
?
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
D-xylose + 1,4-benzoquinone
2-dehydro-D-xylose + 1,4-hydroquinone
-
-
-
-
?
D-xylose + 3-methyl-2-benzothiazolinone hydrazone
2-dehydro-D-xylose + ?
D-xylose + O2
2-dehydro-D-xylose + H2O2
D-xylose + O2
D-threo-pentos-2-ulose + H2O2
D-xylose + O2
D-xylosone + H2O2
ferricenium hexafluorophosphate + O2
? + H2O2
-
-
-
?
gentibiose + O2
2-keto-D-gentibiose + H2O2
L-arabinose + 1,4-benzoquinone
2-dehydro-L-arabinose + 1,4-hydroquinone
-
-
-
-
?
L-arabinose + O2
2-dehydro-L-arabinose + H2O2
-
-
-
-
?
L-sorbose + 3-methyl-2-benzothiazolinone hydrazone
?
L-sorbose + O2
2-dehydro-L-sorbose + H2O2
L-sorbose + O2
5-dehydro-D-fructose + H2O2
L-sorbose D-allose + O2
?
-
-
-
?
lactose + O2
2-dehydrolactose + H2O2
-
0.71% activity compared to D-glucose
-
-
?
maltose + O2
2-dehydro-D-maltose + H2O2
-
92.2% activity compared to D-glucose
-
-
?
methyl alpha-D-glucoside + O2
2-dehydro-alpha-D-methylglucoside + H2O2
methyl beta-D-glucoside + O2
2-dehydro-beta-D-methylglucoside + H2O2
methyl-beta-D-glucoside + O2
?
-
-
-
?
tetrafluoro-1,4-benzoquinone + O2
? + H2O2
trehalose + O2
?
-
14.3% of the activity with D-glucose
-
-
?
wheat flour + O2
?
-
-
-
?
additional information
?
-
2,6-dimethyl-1,4-benzoquinone + O2

? + H2O2
-
-
-
?
2,6-dimethyl-1,4-benzoquinone + O2
? + H2O2
-
-
-
-
?
2-deoxy-D-glucose + O2

2-deoxy-3-keto-D-glucose + H2O2
-
-
-
?
2-deoxy-D-glucose + O2
2-deoxy-3-keto-D-glucose + H2O2
-
25% relative activity to D-glucose
-
?
2-deoxy-D-glucose + O2
2-deoxy-3-keto-D-glucose + H2O2
-
18% relative activity to D-glucose
-
?
2-deoxy-D-glucose + O2
2-deoxy-3-keto-D-glucose + H2O2
-
18% relative activity to D-glucose
-
?
2-deoxy-D-glucose + O2
2-deoxy-3-keto-D-glucose + H2O2
Phanerochaete gigantea
-
1% relative activity to D-glucose
yield: 75%
?
2-keto-D-glucose + O2

2,3-diketo-D-glucose + H2O2
-
-
-
?
2-keto-D-glucose + O2
2,3-diketo-D-glucose + H2O2
-
-
-
?
2-keto-D-glucose + O2
2,3-diketo-D-glucose + H2O2
-
-
-
?
3-deoxy-3-fluoro-beta-D-glucose + H2O2

?
slow substrate
-
-
?
3-deoxy-3-fluoro-beta-D-glucose + H2O2
?
slow substrate
-
-
?
3-deoxy-D-glucose + O2

2-keto-3-deoxy-D-glucose + H2O2
Phanerochaete gigantea
-
96% relative activity to D-glucose
-
?
3-deoxy-D-glucose + O2
2-keto-3-deoxy-D-glucose + H2O2
-
-
-
?
5-thioglucose + O2

2-keto-5-thioglucose + H2O2
-
30% relative activity to D-glucose
-
?
5-thioglucose + O2
2-keto-5-thioglucose + H2O2
-
24% relative activity to D-glucose
-
?
6-deoxy-D-glucose + O2

2-keto-6-deoxy-D-glucose + H2O2
-
-
-
?
6-deoxy-D-glucose + O2
2-keto-6-deoxy-D-glucose + H2O2
-
-
-
?
6-deoxy-D-glucose + O2
2-keto-6-deoxy-D-glucose + H2O2
-
-
-
?
6-deoxy-D-glucose + O2
2-keto-6-deoxy-D-glucose + H2O2
-
76% relative activity to D-glucose
-
?
6-deoxy-D-glucose + O2
2-keto-6-deoxy-D-glucose + H2O2
-
73% relative activity to D-glucose
-
?
6-deoxy-D-glucose + O2
2-keto-6-deoxy-D-glucose + H2O2
-
73% relative activity to D-glucose
-
?
6-deoxy-D-glucose + O2
2-keto-6-deoxy-D-glucose + H2O2
Phanerochaete gigantea
-
15% relative activity to D-glucose
-
?
6-deoxy-D-glucose + O2
2-keto-6-deoxy-D-glucose + H2O2
-
-
-
?
alpha-D-glucose + O2

2-dehydro-D-glucose + H2O2
Coriolus sp.
-
-
-
-
?
alpha-D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
-
?
alpha-D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
-
?
alpha-D-melibiose + O2

? + H2O2
-
-
-
?
alpha-D-melibiose + O2
? + H2O2
-
1.9% relative activity to D-glucose
-
?
alpha-D-melibiose + O2
? + H2O2
-
-
-
?
beta-D-glucose + O2

beta-2-dehydro-D-glucose + H2O2
-
-
-
-
r
beta-D-glucose + O2
beta-2-dehydro-D-glucose + H2O2
-
-
-
-
r
D-allose + O2

?
-
-
-
-
?
D-cellobiose + O2

?
17% of the activity with D-glucose
-
-
?
D-cellobiose + O2
?
17% of the activity with D-glucose
-
-
?
D-cellobiose + O2
?
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
D-fructose + O2

2-dehydro-D-fructose + H2O2
-
-
-
-
?
D-fructose + O2
2-dehydro-D-fructose + H2O2
-
12.4% activity compared to D-glucose
-
-
?
D-fructose + O2

? + H2O2
-
-
-
?
D-fructose + O2
? + H2O2
-
6.5% relative activity to D-glucose
-
?
D-fucono-1,5-lactone + O2

2-keto-D-gluconate + D-araboascorbate + H2O2
-
-
-
-
?
D-fucono-1,5-lactone + O2
2-keto-D-gluconate + D-araboascorbate + H2O2
-
-
-
-
?
D-fucono-1,5-lactone + O2
2-keto-D-gluconate + D-araboascorbate + H2O2
Polyporus obtusus
-
-
-
-
?
D-fucono-1,5-lactone + O2
2-keto-D-gluconate + D-araboascorbate + H2O2
Polyporus obtusus
-
-
-
?
D-fucono-1,5-lactone + O2
2-keto-D-gluconate + D-araboascorbate + H2O2
Polyporus obtusus
-
-
-
-
?
D-fucono-1,5-lactone + O2
2-keto-D-gluconate + D-araboascorbate + H2O2
Polyporus obtusus
-
-
-
?
D-fucose + O2

?
-
2.9% relative activity to D-glucose
-
-
?
D-fucose + O2
?
-
-
-
-
?
D-fucose + O2
?
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
D-galactose + 1,4-benzoquinone

2-dehydro-D-galactose + 1,4-hydroquinone
-
-
-
?
D-galactose + 1,4-benzoquinone
2-dehydro-D-galactose + 1,4-hydroquinone
-
-
-
-
?
D-galactose + 1,4-benzoquinone
2-dehydro-D-galactose + 1,4-hydroquinone
-
-
-
-
?
D-galactose + 1,4-benzoquinone
2-dehydro-D-galactose + 1,4-hydroquinone
-
-
-
-
?
D-galactose + 1,4-benzoquinone
2-dehydro-D-galactose + 1,4-hydroquinone
-
-
-
?
D-galactose + 1,4-benzoquinone
2-dehydro-D-galactose + 1,4-hydroquinone
-
-
-
-
?
D-galactose + 1,4-benzoquinone
2-dehydro-D-galactose + 1,4-hydroquinone
-
-
-
?
D-galactose + O2

2-dehydro-D-galactose + H2O2
-
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
38.8% activity compared to D-glucose
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
38.8% activity compared to D-glucose
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
5% relative activity to D-glucose
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
5% relative activity to D-glucose
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
4% relative activity to D-glucose
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
7.7% relative activity to D-glucose
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
8.5% relative activity to D-glucose
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
5.7% relative activity to D-glucose
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
His-tagged recombinant wild type enzyme strongly prefers D-glucose to D-galactose as its substrate
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
mutants T169S, T169N, and T169G
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
-
0.9% relative activity to D-glucose
-
?
D-galactose + O2

?
-
-
-
-
?
D-galactose + O2
?
-
-
-
?
D-galactose + O2
?
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
D-galactose + O2
?
-
5.41% of the activity with D-glucose
-
-
?
D-glucono-1,5-lactone + 3-methyl-2-benzothiazolinone hydrazone

2-dehydro-D-glucono-1,5-lactone
-
-
-
?
D-glucono-1,5-lactone + 3-methyl-2-benzothiazolinone hydrazone
2-dehydro-D-glucono-1,5-lactone
-
-
-
?
D-glucono-1,5-lactone + O2

?
68% of the activity with D-glucose
-
-
?
D-glucono-1,5-lactone + O2
?
68% of the activity with D-glucose
-
-
?
D-gluconolactone + O2

? + H2O2
-
activity about 10% that of D-glucose
-
?
D-gluconolactone + O2
? + H2O2
-
60% relative activity to D-glucose
-
?
D-gluconolactone + O2
? + H2O2
-
60% relative activity to D-glucose
-
?
D-gluconolactone + O2
? + H2O2
-
44% relative activity to D-glucose
-
?
D-gluconolactone + O2
? + H2O2
-
1.5% relative activity to D-glucose
-
?
D-gluconolactone + O2
? + H2O2
Polyporus obtusus
-
-
-
?
D-gluconolactone + O2
? + H2O2
Polyporus obtusus
-
14% relative activity to D-glucose, two main products, i.e., 2-ketogluconic acid and araboascorbic acid are formed
-
?
D-gluconolactone + O2
? + H2O2
-
64% relative activity to D-glucose
-
?
D-glucose + 1,4-benzoquinone

2-dehydro-D-glucose + 1,4-hydroquinone
-
-
-
?
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + 1,4-hydroquinone
-
-
-
?
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + 1,4-hydroquinone
-
-
-
-
?
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + 1,4-hydroquinone
-
-
-
-
?
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + 1,4-hydroquinone
-
-
-
?
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + 1,4-hydroquinone
-
-
-
-
?
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + 1,4-hydroquinone
-
-
-
?
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + 1,4-hydroquinone
-
-
-
-
?
D-glucose + 1,4-benzoquinone

?
-
-
-
-
?
D-glucose + 1,4-benzoquinone
?
-
-
-
-
?
D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical

2-dehydro-D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
-
-
-
?
D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical
2-dehydro-D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
-
-
?
D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical
2-dehydro-D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
-
-
-
?
D-glucose + 3-methyl-2-benzothiazolinone hydrazone

2-dehydro-D-glucose + ?
-
-
-
?
D-glucose + 3-methyl-2-benzothiazolinone hydrazone
2-dehydro-D-glucose + ?
-
-
-
?
D-glucose + ferricenium hexafluorophosphate

?
-
-
-
-
?
D-glucose + ferricenium hexafluorophosphate
?
-
-
-
-
?
D-glucose + ferricenium ion

2-dehydro-D-glucose + ferrocenium ion
-
-
-
?
D-glucose + ferricenium ion
2-dehydro-D-glucose + ferrocenium ion
-
-
-
r
D-glucose + O2

2-dehydro-D-glucose + H2O2
-
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
Coriolus sp.
-
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
preferred substrate
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
100% activity
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
preferred substrate
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
100% activity
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
D-glucose is the best substrate
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
D-glucose is the best substrate
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
656544, 695870, 696791, 699076, 711239, 712500, 724369, 724753, 724979, 725776, 741113, 741252 -
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
-
r
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
immobilized and soluble pyranose 2-oxidase analyzed
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
recombinant pyranose 2-oxidase analyzed
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
His-tagged recombinant wild type enzyme strongly prefers D-glucose to D-galactose as its substrate
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
the wild type enzyme displays a clear preference for D-glucose over D-galactose
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
highly regioselective mechanism
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
recombinant pyranose 2-oxidase analyzed
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
-
-
-
-
?
D-glucose + O2

D-arabino-hexos-2-ulose + H2O2
Polyporus obtusus
-
-
the product is a specific tautomeric form of D-arabino-hexos-2-ulose (form IV, 20%) being in equilibrium with three other forms of D-arabinohexos-2-ulose in solution
-
?
D-glucose + O2
D-arabino-hexos-2-ulose + H2O2
Polyporus obtusus AU124PD
-
-
the product is a specific tautomeric form of D-arabino-hexos-2-ulose (form IV, 20%) being in equilibrium with three other forms of D-arabinohexos-2-ulose in solution
-
?
D-glucose + O2

D-glucosone + H2O2
-
-
-
?
D-glucose + O2
D-glucosone + H2O2
-
-
-
?
D-glucose + O2
D-glucosone + H2O2
-
-
D-glucosone is identical with D-arabino-2-hexosulose
?
D-glucose + O2
D-glucosone + H2O2
-
-
-
?
D-glucose + O2
D-glucosone + H2O2
-
D-glucose is the preferred substrate
-
?
D-glucose + O2
D-glucosone + H2O2
-
D-glucose is the preferred substrate
-
?
D-glucose + O2
D-glucosone + H2O2
-
D-glucose is the preferred substrate
-
?
D-glucose + O2
D-glucosone + H2O2
Corticium caeruleum
-
-
-
?
D-glucose + O2
D-glucosone + H2O2
-
-
-
?
D-glucose + O2
D-glucosone + H2O2
-
-
-
?
D-glucose + O2
D-glucosone + H2O2
-
-
D-glucosone is identical with D-arabino-2-hexosulose
?
D-glucose + O2
D-glucosone + H2O2
-
-
D-glucosone is identical with D-arabino-2-hexosulose
?
D-glucose + O2
D-glucosone + H2O2
-
-
-
?
D-glucose + O2
D-glucosone + H2O2
-
-
-
?
D-glucose + O2
D-glucosone + H2O2
-
-
-
?
D-glucose + O2
D-glucosone + H2O2
-
D-glucose is the preferred substrate
-
?
D-glucose + O2
D-glucosone + H2O2
-
D-glucose is the preferred substrate
-
?
D-glucose + O2
D-glucosone + H2O2
-
D-glucose is the preferred substrate
D-glucosone is identical with D-arabino-2-hexosulose
?
D-glucose + O2
D-glucosone + H2O2
-
D-glucose is the preferred substrate
D-glucosone is identical with D-arabino-2-hexosulose
?
D-glucose + O2
D-glucosone + H2O2
-
alpha-D-glucose has a 104% relative activity compared to D-glucose, beta-D-glucose has a 80% relative activity compared to D-glucose
-
?
D-glucose + O2
D-glucosone + H2O2
Phanerochaete gigantea
-
-
-
?
D-glucose + O2
D-glucosone + H2O2
-
100% relative activity
-
?
D-glucose + O2
D-glucosone + H2O2
-
the activation energy for D-glucose activation is 24.7 kJ/mol
-
?
D-glucose + O2
D-glucosone + H2O2
-
activation energy for the conversion of D-glucose is 34.6 kJ/mol
-
?
D-glucose + O2
D-glucosone + H2O2
-
D-glucose is the preferred substrate
-
?
D-glucose + O2
D-glucosone + H2O2
-
D-glucose is the preferred substrate
-
?
D-glucose + O2
D-glucosone + H2O2
-
D-glucose is the preferred substrate
-
?
D-glucose + O2
D-glucosone + H2O2
-
-
-
?
D-glucose + O2
D-glucosone + H2O2
Polyporus obtusus
-
-
-
?
D-glucose + O2
D-glucosone + H2O2
Polyporus obtusus
-
-
D-glucosone is identical with D-arabino-2-hexosulose
?
D-glucose + O2
D-glucosone + H2O2
Polyporus obtusus
-
other electron acceptors: 2,6-dichlorophenolindophenol, cytochrome c
D-glucosone is identical with D-arabino-2-hexosulose
?
D-glucose + O2
D-glucosone + H2O2
Polyporus obtusus
-
D-glucose is the preferred substrate
-
?
D-glucose + O2
D-glucosone + H2O2
Polyporus obtusus
-
D-glucose is the preferred substrate
-
?
D-glucose + O2
D-glucosone + H2O2
Polyporus obtusus
-
D-glucose is the preferred substrate
D-glucosone is identical with D-arabino-2-hexosulose
?
D-glucose + O2
D-glucosone + H2O2
Polyporus obtusus
-
D-glucose is the preferred substrate
D-glucosone is identical with D-arabino-2-hexosulose
?
D-glucose + O2
D-glucosone + H2O2
Saxidomus giganteus
-
-
-
?
D-glucose + O2
D-glucosone + H2O2
-
-
-
?
D-glucose + O2
D-glucosone + H2O2
Trametes cinnabarinus
-
-
D-glucosone is identical with D-arabino-2-hexosulose
?
D-glucose + O2
D-glucosone + H2O2
-
-
-
?
D-glucose + O2
D-glucosone + H2O2
-
100% relative activity
-
?
D-glucose + O2
D-glucosone + H2O2
-
D-glucose is the preferred substrate
-
?
D-glucose + O2
D-glucosone + H2O2
-
-
-
-
?
D-glucose + O2
D-glucosone + H2O2
-
-
D-glucosone is identical with D-arabino-2-hexosulose
?
D-glucose + O2
D-glucosone + H2O2
-
both alpha- and beta-anomeric forms can serve almost equally as substrate
-
?
D-glucose + O2
D-glucosone + H2O2
-
other electron acceptors: 2,6-dichlorophenolindophenol, cytochrome c
-
?
D-glucose + O2
D-glucosone + H2O2
-
D-glucose is the preferred substrate
-
?
D-glucose + O2
D-glucosone + H2O2
-
D-glucose is the preferred substrate
-
?
D-glucose + O2
D-glucosone + H2O2
-
D-glucose is the preferred substrate
D-glucosone is identical with D-arabino-2-hexosulose
?
D-glucose + O2
D-glucosone + H2O2
-
-
-
-
?
D-melibiose + O2

?
-
-
-
-
?
D-melibiose + O2
?
-
-
-
?
D-xylose + 3-methyl-2-benzothiazolinone hydrazone

2-dehydro-D-xylose + ?
-
-
-
?
D-xylose + 3-methyl-2-benzothiazolinone hydrazone
2-dehydro-D-xylose + ?
-
-
-
?
D-xylose + O2

2-dehydro-D-xylose + H2O2
-
-
-
-
?
D-xylose + O2
2-dehydro-D-xylose + H2O2
-
-
-
-
?
D-xylose + O2
2-dehydro-D-xylose + H2O2
-
75.1% activity compared to D-glucose
-
-
?
D-xylose + O2
2-dehydro-D-xylose + H2O2
-
-
-
-
?
D-xylose + O2
2-dehydro-D-xylose + H2O2
-
-
-
?
D-xylose + O2
2-dehydro-D-xylose + H2O2
-
-
-
?
D-xylose + O2

?
-
-
-
?
D-xylose + O2
?
35% of the activity with D-glucose
-
-
?
D-xylose + O2
?
35% of the activity with D-glucose
-
-
?
D-xylose + O2
?
-
-
-
-
?
D-xylose + O2
?
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
D-xylose + O2
?
-
13.4% of the activity with D-glucose
-
-
?
D-xylose + O2

D-threo-pentos-2-ulose + H2O2
Polyporus obtusus
-
-
-
-
?
D-xylose + O2
D-threo-pentos-2-ulose + H2O2
Polyporus obtusus AU124PD
-
-
-
-
?
D-xylose + O2

D-xylosone + H2O2
-
-
-
?
D-xylose + O2
D-xylosone + H2O2
-
-
-
?
D-xylose + O2
D-xylosone + H2O2
-
activity about 10% that of D-glucose
-
?
D-xylose + O2
D-xylosone + H2O2
Coriolus sp.
-
-
-
-
?
D-xylose + O2
D-xylosone + H2O2
-
-
-
?
D-xylose + O2
D-xylosone + H2O2
-
-
-
?
D-xylose + O2
D-xylosone + H2O2
-
-
-
?
D-xylose + O2
D-xylosone + H2O2
-
-
-
?
D-xylose + O2
D-xylosone + H2O2
-
30% relative activity to D-glucose
-
?
D-xylose + O2
D-xylosone + H2O2
-
37% relative activity to D-glucose
-
?
D-xylose + O2
D-xylosone + H2O2
-
37% relative activity to D-glucose
-
?
D-xylose + O2
D-xylosone + H2O2
-
-
-
?
D-xylose + O2
D-xylosone + H2O2
-
50% relative activity to D-glucose
-
?
D-xylose + O2
D-xylosone + H2O2
-
43.7% relative activity to D-glucose
-
?
D-xylose + O2
D-xylosone + H2O2
Polyporus obtusus
-
-
-
?
D-xylose + O2
D-xylosone + H2O2
Polyporus obtusus
-
-
-
?
D-xylose + O2
D-xylosone + H2O2
Polyporus obtusus
-
-
-
?
D-xylose + O2
D-xylosone + H2O2
Polyporus obtusus
-
-
-
?
D-xylose + O2
D-xylosone + H2O2
Polyporus obtusus
-
38% relative activity to D-glucose
-
?
D-xylose + O2
D-xylosone + H2O2
-
56% relative activity to D-glucose
-
?
D-xylose + O2
D-xylosone + H2O2
-
-
-
?
D-xylose + O2
D-xylosone + H2O2
-
-
-
?
D-xylose + O2
D-xylosone + H2O2
-
2.6% relative activity to D-glucose
-
?
gentibiose + O2

2-keto-D-gentibiose + H2O2
-
-
-
?
gentibiose + O2
2-keto-D-gentibiose + H2O2
-
-
-
?
gentibiose + O2
2-keto-D-gentibiose + H2O2
-
23% relative activity to D-glucose
-
?
L-arabinose + O2

?
-
1.5% relative activity to D-glucose
-
-
?
L-arabinose + O2
?
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
L-sorbose + 3-methyl-2-benzothiazolinone hydrazone

?
-
-
-
?
L-sorbose + 3-methyl-2-benzothiazolinone hydrazone
?
-
-
-
?
L-sorbose + O2

2-dehydro-L-sorbose + H2O2
-
-
-
-
?
L-sorbose + O2
2-dehydro-L-sorbose + H2O2
-
-
-
-
?
L-sorbose + O2
2-dehydro-L-sorbose + H2O2
-
122% activity compared to D-glucose
-
-
?
L-sorbose + O2

5-dehydro-D-fructose + H2O2
-
-
-
?
L-sorbose + O2
5-dehydro-D-fructose + H2O2
-
activity about 10% that of D-glucose
-
?
L-sorbose + O2
5-dehydro-D-fructose + H2O2
Coriolus sp.
-
-
-
-
?
L-sorbose + O2
5-dehydro-D-fructose + H2O2
-
-
-
?
L-sorbose + O2
5-dehydro-D-fructose + H2O2
-
-
-
?
L-sorbose + O2
5-dehydro-D-fructose + H2O2
-
68% relative activity to D-glucose
-
?
L-sorbose + O2
5-dehydro-D-fructose + H2O2
-
52% relative activity to D-glucose
-
?
L-sorbose + O2
5-dehydro-D-fructose + H2O2
-
52% relative activity to D-glucose
-
?
L-sorbose + O2
5-dehydro-D-fructose + H2O2
-
96.3% relative activity to D-glucose
-
?
L-sorbose + O2
5-dehydro-D-fructose + H2O2
-
109% relative activity to D-glucose
-
?
L-sorbose + O2
5-dehydro-D-fructose + H2O2
Polyporus obtusus
-
-
-
?
L-sorbose + O2
5-dehydro-D-fructose + H2O2
Polyporus obtusus
-
-
-
?
L-sorbose + O2
5-dehydro-D-fructose + H2O2
Polyporus obtusus
-
-
-
?
L-sorbose + O2
5-dehydro-D-fructose + H2O2
Polyporus obtusus
-
59% relative activity to D-glucose
-
?
L-sorbose + O2
5-dehydro-D-fructose + H2O2
-
99% relative activity to D-glucose
-
?
L-sorbose + O2
5-dehydro-D-fructose + H2O2
-
-
-
?
L-sorbose + O2
5-dehydro-D-fructose + H2O2
-
3.4% relative activity to D-glucose
-
?
L-sorbose + O2

?
-
-
-
?
L-sorbose + O2
?
-
-
-
-
?
L-sorbose + O2
?
-
17.9% of the activity with D-glucose
-
-
?
maltose + O2

? + H2O2
-
26% relative activity to D-glucose
-
?
maltose + O2
? + H2O2
-
8.2% relative activity to D-glucose
-
?
methyl alpha-D-glucoside + O2

2-dehydro-alpha-D-methylglucoside + H2O2
-
3% relative activity to D-glucose
-
-
?
methyl alpha-D-glucoside + O2
2-dehydro-alpha-D-methylglucoside + H2O2
-
1.9% relative activity to D-glucose
-
-
?
methyl beta-D-glucoside + O2

2-dehydro-beta-D-methylglucoside + H2O2
-
26.3% relative activity to D-glucose
-
-
?
methyl beta-D-glucoside + O2
2-dehydro-beta-D-methylglucoside + H2O2
-
9.6% relative activity to D-glucose
-
-
?
sucrose + O2

? + H2O2
-
82.8% activity compared to D-glucose
-
-
?
sucrose + O2
? + H2O2
-
82.8% activity compared to D-glucose
-
-
?
tetrafluoro-1,4-benzoquinone + O2

? + H2O2
-
-
-
?
tetrafluoro-1,4-benzoquinone + O2
? + H2O2
-
-
-
-
?
additional information

?
-
-
no activity with mannose and lactose as substrates. The enzyme catalyzes the two-electron reduction of 1,4-benzoquinone, several substituted benzoquinones and 2,6-dichloroindophenol. Some of these quinone electron acceptors are better substrates for pyranose oxidase than oxygen. Peroxidase-coupled assay using [2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid)] as the chromogen
-
-
?
additional information
?
-
-
no activity with D-fructose, mannose and lactose as substrates. Peroxidase-coupled assay using [2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid)] as the chromogen
-
-
?
additional information
?
-
-
no activity with D-arabinose
-
-
?
additional information
?
-
-
no activity with D-arabinose
-
-
?
additional information
?
-
evidence that the enzyme has a role in lignocellulose degradation
-
-
?
additional information
?
-
at 10 mM concentration, glucose is the best substrate, but xylose, sorbose, and D-glucono-1,5-lactone have more than half of its activity
-
-
?
additional information
?
-
evidence that the enzyme has a role in lignocellulose degradation
-
-
?
additional information
?
-
-
evidence that the enzyme has a role in lignocellulose degradation
-
-
?
additional information
?
-
at 10 mM concentration, glucose is the best substrate, but xylose, sorbose, and D-glucono-1,5-lactone have more than half of its activity
-
-
?
additional information
?
-
-
no activity in the presence of D-mannose, D-fructose, maltose, trehalose, lactose and D-melibiose
-
-
?
additional information
?
-
-
no activity in the presence of D-mannose, D-fructose, maltose, trehalose, lactose and D-melibiose
-
-
?
additional information
?
-
-
pyranose 2-oxidase from Trametes multicolor is a flavoenzyme that catalyzes the oxidation of D-glucose and other aldopyranose sugars at the C2 position by using O2 as an electron acceptor to form the corresponding 2-oxo-sugars and H2O2
-
-
?
additional information
?
-
ferulic acid oxidation in a model system and of thiol oxidation in a wheat flour extract, overview. L-arabinose is a poor substrate. Dehydroascorbic acid does not act as electron acceptor
-
-
?
additional information
?
-
-
most of the catalytic dehydrogenation of substrates by flavoprotein oxidases in the GMC class is initiated by the removal of a hydroxyl proton followed by the transfer of a hydride moiety. Pyranose 2-oxidase catalyzes the oxidation of several aldopyranoses by molecular oxygen at the C2 position to yield the corresponding 2-keto-aldoses and hydrogen peroxide
-
-
?
additional information
?
-
ferulic acid oxidation in a model system and of thiol oxidation in a wheat flour extract, overview. L-arabinose is a poor substrate. Dehydroascorbic acid does not act as electron acceptor
-
-
?
additional information
?
-
-
the rate of bioconversion of D-glucose by glucose 2-oxidase in the presence of either nitrogen or supercritical CO2 at 110 bar is very low compared with the use of compressed air at the same pressure
-
-
?
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0.0052 - 2
1,4-benzoquinone
10.7
1,5-anhydro-D-glucitol
-
pH 7.0, 37°C
0.07 - 0.09
2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical
0.051 - 0.39
2,6-dichloroindophenol
0.065 - 0.094
2,6-dichlorophenolindophenol
0.4 - 2.1
2,6-dimethyl-1,4-benzoquinone
0.31 - 0.48
2-chloro-1,4-benzoquinone
10.32
2-deoxy-D-galactose
100 mM ethanolamine buffer, pH 10.5
11.3 - 99.3
2-deoxy-D-glucose
0.21 - 0.42
2-methoxy-1,4-benzoquinone
0.11 - 0.51
2-methyl-1,4-benzoquinone
120
6-O-alpha-D-galactopyranosyl-beta-D-glucopyranose
-
-
0.57 - 0.97
beta-D-glucose
290
cellobiose
-
at pH 6.5 and 25°C
174
D-arabinose
-
pH 7.0, 37°C
0.71
D-cellobiose
100 mM ethanolamine buffer, pH 10.5
6.25
D-fucose
100 mM ethanolamine buffer, pH 10.5
51.1
D-glucono-1,5-lactone
-
at pH 6.5 and 25°C
6.02
D-maltoheptaose
100 mM ethanolamine buffer, pH 10.5
6.14
D-maltopentaose
100 mM ethanolamine buffer, pH 10.5
329
D-maltose
-
pH 7.0, 37°C
6.61
D-maltotriose
100 mM ethanolamine buffer, pH 10.5
180
D-ribose
-
with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C
7.51
D-trehalose
100 mM ethanolamine buffer, pH 10.5
0.092 - 1.12
ferricenium hexafluorophosphate
0.054 - 0.408
ferricenium ion
3.43
ferricyanide
substrate ferricyanide (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
0.015 - 0.4
ferrocenium hexafluorophosphate
0.187
ferrocenium ion
using D-glucose as cosubstrate, at 30°C, pH 6.5
72.8
melibiose
at 30°C, pH 6.5
0.35 - 0.4
methyl-1,4-benzoquinone
50 - 289
methyl-beta-D-glucoside
902
sucrose
-
at pH 6.5 and 25°C
0.09
tetrabromo-1,4-benzoquinone
0.088
tetrachloro-1,4-benzoquinone
-
pH 6.5, substrate: D-glucose
0.22 - 9.42
tetrafluoro-1,4-benzoquinone
192
trehalose
-
pH 7.0, 37°C
additional information
additional information
-
0.0052
1,4-benzoquinone

mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
0.0071
1,4-benzoquinone
mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
0.0089
1,4-benzoquinone
mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
0.01
1,4-benzoquinone
mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
0.013
1,4-benzoquinone
mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
0.027
1,4-benzoquinone
wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
0.029
1,4-benzoquinone
mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
0.029
1,4-benzoquinone
mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
0.032
1,4-benzoquinone
L537W/E542R mutant, substrate 1,4-benzoquinone (D-galactose concentration constant, 100 mM), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.033
1,4-benzoquinone
using D-glucose as cosubstrate, at 30°C, pH 6.5
0.036
1,4-benzoquinone
L537W mutant, substrate 1,4-benzoquinone (D-galactose concentration constant, 100 mM), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.037
1,4-benzoquinone
L537G/E542R mutant, substrate 1,4-benzoquinone (D-galactose concentration constant, 100 mM), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.038
1,4-benzoquinone
L537W/E542K mutant, substrate 1,4-benzoquinone (D-galactose concentration constant, 100 mM), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.04
1,4-benzoquinone
E542R mutant, substrate 1,4-benzoquinone (D-galactose concentration constant, 100 mM), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.04
1,4-benzoquinone
L537G/E542K mutant, substrate 1,4-benzoquinone (D-galactose concentration constant, 100 mM), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.042
1,4-benzoquinone
substrate 1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 4.5
0.043
1,4-benzoquinone
-
V546C/T169G mutant, substrate 1,4-benzoquinone (constant D-glucose concentration, 100 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
0.048
1,4-benzoquinone
L537G mutant, substrate 1,4-benzoquinone (D-galactose concentration constant, 100 mM), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.049
1,4-benzoquinone
E542K mutant, substrate 1,4-benzoquinone (D-galactose concentration constant, 100 mM), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.052
1,4-benzoquinone
mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
0.065
1,4-benzoquinone
wild-type, substrate 1,4-benzoquinone (D-galactose concentration constant, 100 mM), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.072
1,4-benzoquinone
-
V546C/T169G/L537W mutant, substrate 1,4-benzoquinone (constant D-glucose concentration, 100 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
0.072
1,4-benzoquinone
mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
0.072
1,4-benzoquinone
mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
0.078
1,4-benzoquinone
mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
0.11
1,4-benzoquinone
substrate 1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
0.12
1,4-benzoquinone
-
with D-glucose as cosubstrate, at pH 6.5 and 37°C
0.13
1,4-benzoquinone
L537W mutant, substrate 1,4-benzoquinone (D-glucose concentration constant, 100 mM), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.136
1,4-benzoquinone
E542R mutant, substrate 1,4-benzoquinone (D-glucose concentration constant, 100 mM), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.137
1,4-benzoquinone
L537W/E542R mutant, substrate 1,4-benzoquinone (D-glucose concentration constant, 100 mM), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.14
1,4-benzoquinone
L537W/E542K mutant, substrate 1,4-benzoquinone (D-glucose concentration constant, 100 mM), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.14
1,4-benzoquinone
wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
0.15
1,4-benzoquinone
L537G/E542K mutant, substrate 1,4-benzoquinone (D-glucose concentration constant, 100 mM), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.15
1,4-benzoquinone
-
pH 6.5, 30°C, recombinant enzyme
0.155
1,4-benzoquinone
L537G/E542R mutant, substrate 1,4-benzoquinone (D-glucose concentration constant, 100 mM), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.176
1,4-benzoquinone
L537G mutant, substrate 1,4-benzoquinone (D-glucose concentration constant, 100 mM), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.182
1,4-benzoquinone
E542K mutant, substrate 1,4-benzoquinone (D-glucose concentration constant, 100 mM), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.24
1,4-benzoquinone
mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
0.241
1,4-benzoquinone
wild-type, substrate 1,4-benzoquinone (D-glucose concentration constant, 100 mM), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.28
1,4-benzoquinone
-
V546C/T169G mutant, substrate 1,4-benzoquinone (constant D-galactose concentration, 100 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
0.3
1,4-benzoquinone
-
pH 4.5, substrate: D-glucose
0.3
1,4-benzoquinone
-
soluble enzyme, 500 mM glucose used as electron acceptor, pH 5.0
0.31
1,4-benzoquinone
-
pH 6.5, substrate: D-glucose
0.32
1,4-benzoquinone
-
pH 6.5, 30°C, recombinant enzyme
0.37
1,4-benzoquinone
-
V546C/E542K mutant, substrate 1,4-benzoquinone (constant D-glucose concentration, 100 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
1.18
1,4-benzoquinone
-
V546C/T169G/L537W mutant, substrate 1,4-benzoquinone (constant D-galactose concentration, 100 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
1.5 - 2
1,4-benzoquinone
-
V546C/E542K mutant, substrate 1,4-benzoquinone (constant D-galactose concentration, 100 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
0.07
2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical

-
pH 6.5, substrate: D-glucose
0.09
2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical
substrate 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
0.051
2,6-dichloroindophenol

substrate 2,6-dichloroindophenol (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
0.187
2,6-dichloroindophenol
using D-glucose as cosubstrate, at 30°C, pH 6.5
0.39
2,6-dichloroindophenol
-
pH 6.5, 30°C, recombinant enzyme
0.065
2,6-dichlorophenolindophenol

-
pH 6.5, substrate: D-glucose
0.065
2,6-dichlorophenolindophenol
-
at pH 6.5 and 30°C
0.094
2,6-dichlorophenolindophenol
-
pH 4.5, substrate: D-glucose
0.094
2,6-dichlorophenolindophenol
-
at pH 4.5 and 30°C
0.4
2,6-dimethyl-1,4-benzoquinone

-
pH 6.5, 30°C, recombinant enzyme
0.83
2,6-dimethyl-1,4-benzoquinone
-
pH 4.5, substrate: D-glucose
1.59
2,6-dimethyl-1,4-benzoquinone
substrate 2,6-dimethyl-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
2.1
2,6-dimethyl-1,4-benzoquinone
-
pH 6.5, substrate: D-glucose
0.31
2-chloro-1,4-benzoquinone

substrate 2-chloro-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
0.48
2-chloro-1,4-benzoquinone
-
pH 6.5, 30°C, recombinant enzyme
11.3
2-deoxy-D-glucose

pH 7.3, 22°C, wild-type enzyme
11.85
2-deoxy-D-glucose
100 mM ethanolamine buffer, pH 10.5
55
2-deoxy-D-glucose
-
oxygen concentration 1.07 mM
79
2-deoxy-D-glucose
pH 7.3, 22°C, mutant enzyme E540K with a C-terminal His6-tag
99.3
2-deoxy-D-glucose
pH 7.3, 22°C, mutant enzyme K312E with a C-terminal His6-tag
0.21
2-methoxy-1,4-benzoquinone

substrate 2-methoxy-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
0.42
2-methoxy-1,4-benzoquinone
-
pH 6.5, 30°C, recombinant enzyme
0.11
2-methyl-1,4-benzoquinone

-
pH 6.5, 30°C, recombinant enzyme
0.51
2-methyl-1,4-benzoquinone
substrate 2-methyl-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
1
alpha-D-glucose

-
-
1.53
alpha-D-glucose
-
oxygen concentration 1.07 mM
0.57
beta-D-glucose

-
-
0.97
beta-D-glucose
-
oxygen concentration 1.07 mM
22.1
D-allose

pH 7.3, 22°C, mutant enzyme E540K with a C-terminal His6-tag
22.3
D-allose
pH 7.3, 22°C, mutant enzyme E540K
55.7
D-allose
pH 7.3, 22°C, wild-type enzyme
68
D-allose
pH 7.3, 22°C, mutant enzyme K312E with a C-terminal His6-tag
103
D-fructose

substrate D-fructose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
727
D-fructose
-
at pH 6.5 and 25°C
2558
D-fructose
-
pH 6.5, 30°C, recombinant enzyme
0.093
D-galactose

T169G/E542K/V546C, mutant, 30°C, substrate D-galactose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay
0.19
D-galactose
T169G/E542K/V546C, mutant, 50°C, substrate D-galactose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay
0.23
D-galactose
wild-type, mutant, 50°C, substrate D-galactose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay
0.25
D-galactose
wild-type, mutant, 30°C, substrate D-galactose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay
0.4
D-galactose
-
V546C/T169G mutant, substrate D-galactose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
0.4
D-galactose
mutant enzyme T196G/V546C, pH and temperature not specified in the publication
0.86
D-galactose
-
V546C/T169G/L537W mutant, substrate D-galactose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
0.983
D-galactose
V546P/T169G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
1.66
D-galactose
T169G/E542K/V546C, mutant, 30°C, substrate D-galactose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay
2.45
D-galactose
mutant enzyme H450G, in 50 mM phosphate buffer (pH 6.5) at 60°C
2.45
D-galactose
mutant enzyme H450G, pH and temperature not specified in the publication
2.48
D-galactose
T169G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
2.48
D-galactose
mutant enzyme T169G, pH and temperature not specified in the publication
2.76
D-galactose
T169G/E542K/V546C, mutant, 50°C, substrate D-galactose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay
2.94
D-galactose
substrate D-galactose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
3.1
D-galactose
-
mutant enzyme T169G, in 50 mM sodium phosphate (pH 7.0), at 25°C
3.56
D-galactose
T169N, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
3.7
D-galactose
-
pH 7.0, 25°C, reductive half-reaction, wild-type enzyme
3.84
D-galactose
at 30°C, pH 6.5
3.87
D-galactose
E542K mutant, substrate D-galactose (O2 concentration constant), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
3.87
D-galactose
mutant enzyme E542K, in 50 mM phosphate buffer (pH 6.5) at 60°C
4.2
D-galactose
-
pH 7.0, 25°C, reductive half-reaction, mutant T169G
4.26
D-galactose
E542R mutant, substrate D-galactose (O2 concentration constant), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
5.19
D-galactose
L537W/E542K mutant, substrate D-galactose (O2 concentration constant), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
5.2
D-galactose
pH 7.3, 22°C, mutant enzyme E540K
5.49
D-galactose
L537W/E542R mutant, substrate D-galactose (O2 concentration constant), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
5.77
D-galactose
L537G/E542R mutant, substrate D-galactose (O2 concentration constant), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
5.9
D-galactose
-
pH 7.0, 25°C, reductive half-reaction, mutant T169S
6.01
D-galactose
L537G/E542K mutant, substrate D-galactose (O2 concentration constant), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
6.1
D-galactose
pH 7.3, 22°C, mutant enzyme K312E with a C-terminal His6-tag
6.1
D-galactose
wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
6.3
D-galactose
pH 7.3, 22°C, mutant enzyme E540K with a C-terminal His6-tag
6.38
D-galactose
R472G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
6.6
D-galactose
-
pH 7.0, 25°C, reductive half-reaction, mutant T169N
7.21
D-galactose
R472L, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
7.39
D-galactose
N593R, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
7.73
D-galactose
-
at pH 6.5 and 25°C
7.8
D-galactose
mutant enzyme H450G/E542K/V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C
7.94
D-galactose
wild-type, mutant, 30°C, substrate D-galactose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay
8.09
D-galactose
wild-type, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
8.2
D-galactose
pH 7.3, 22°C, wild-type enzyme
8.79
D-galactose
wild-type, substrate D-galactose (O2 concentration constant), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
8.79
D-galactose
His-tagged recombinant wild type enzyme, in 50 mM phosphate buffer (pH 6.5) at 60°C
8.79
D-galactose
wild type enzyme, pH and temperature not specified in the publication
9.27
D-galactose
H548R, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
9.4
D-galactose
L537W mutant, substrate D-galactose (O2 concentration constant), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
9.47
D-galactose
L537G mutant, substrate D-galactose (O2 concentration constant), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
9.89
D-galactose
H548I, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
10
D-galactose
mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
10.4
D-galactose
V546C/T169N, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
11.81
D-galactose
100 mM ethanolamine buffer, pH 10.5
12
D-galactose
mutant enzyme H450G/V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C
12
D-galactose
mutant enzyme H450G/V546C, pH and temperature not specified in the publication
12.9
D-galactose
T169S, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
13
D-galactose
mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
13
D-galactose
mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
13
D-galactose
-
pH 6.5, 30°C, recombinant enzyme
14.6
D-galactose
wild-type, mutant, 50°C, substrate D-galactose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay
15
D-galactose
-
wild type enzyme, in 50 mM sodium phosphate (pH 7.0), at 25°C
16
D-galactose
-
mutant enzyme T169N, in 50 mM sodium phosphate (pH 7.0), at 25°C
16.2
D-galactose
-
mutant enzyme T169S, in 50 mM sodium phosphate (pH 7.0), at 25°C
24.6
D-galactose
-
V546C/E542K mutant, substrate D-galactose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
26
D-galactose
mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
29
D-galactose
mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
29.4
D-galactose
V546G/T169G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
34
D-galactose
mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
45
D-galactose
-
pH 7.0, 37°C
46.2
D-galactose
V546C, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
46.2
D-galactose
mutant enzyme V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C
46.2
D-galactose
mutant enzyme V546C, pH and temperature not specified in the publication
50
D-galactose
-
mutant enzyme T169A, in 50 mM sodium phosphate (pH 7.0), at 25°C
50.6
D-galactose
V546P, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
101
D-galactose
-
with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C
192
D-galactose
-
pH 6.5, 30°C, recombinant enzyme
200
D-galactose
V546G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
750
D-galactose
Q448S, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
940
D-galactose
Q448N, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
1260
D-galactose
Q448C, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
0.018
D-glucose

mutant enzyme L547R, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C
0.042
D-glucose
mutant enzyme L545C, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C
0.046
D-glucose
mutant enzyme L545C, with O2 as electron acceptor, at pH 6.5 and 30°C
0.057
D-glucose
mutant enzyme Q448H, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C
0.082
D-glucose
mutant enzyme Q448H, with O2 as electron acceptor, at pH 6.5 and 30°C
0.092
D-glucose
mutant enzyme T166R, with O2 as electron acceptor, at pH 6.5 and 30°C
0.12
D-glucose
mutant enzyme L545C, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C
0.13
D-glucose
wild type enzyme, with O2 as electron acceptor, at pH 6.5 and 30°C
0.14
D-glucose
mutant enzyme N593C, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C
0.14
D-glucose
mutant enzyme T166R, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C
0.18
D-glucose
mutant enzyme N593C, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C
0.22
D-glucose
T169G/E542K/V546C, mutant, 30°C, substrate D-glucose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay
0.23
D-glucose
mutant enzyme L547R, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C
0.24
D-glucose
mutant enzyme Q448H, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C
0.24
D-glucose
wild type enzyme, with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 30°C
0.25
D-glucose
wild type enzyme, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C
0.31
D-glucose
T169G/E542K/V546C, mutant, 50°C, substrate D-glucose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay
0.314
D-glucose
at 30°C, pH 6.5
0.32
D-glucose
wild-type protein, 0.1 M ethanolamine buffer at pH 10.5
0.327
D-glucose
T169N, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
0.35
D-glucose
recombinant protein with a hexa-histidine tag, 0.1 M ethanolamine buffer at pH 10.5
0.394
D-glucose
T169S, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
0.4
D-glucose
pH 7.3, 22°C, mutant enzyme K312E with a C-terminal His6-tag
0.4
D-glucose
wild-type, mutant, 30°C, substrate D-glucose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay
0.4
D-glucose
mutant enzyme L547R, with O2 as electron acceptor, at pH 6.5 and 30°C
0.419
D-glucose
L537W/E542R mutant, substrate D-glucose (O2 concentration constant), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.432
D-glucose
L537W/E542K mutant, substrate D-glucose (O2 concentration constant), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.44
D-glucose
-
V546C/T169G mutant, substrate D-glucose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
0.44
D-glucose
mutant enzyme T196G/V546C, pH and temperature not specified in the publication
0.441
D-glucose
L537G/E542R mutant, substrate D-glucose (O2 concentration constant), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.487
D-glucose
L537G/E542K mutant, substrate D-glucose (O2 concentration constant), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.489
D-glucose
E542R mutant, substrate D-glucose (O2 concentration constant), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.49
D-glucose
-
V546C/T169G/L537W mutant, substrate D-glucose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
0.521
D-glucose
E542K mutant, substrate D-glucose (O2 concentration constant), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.521
D-glucose
mutant enzyme E542K, in 50 mM phosphate buffer (pH 6.5) at 60°C
0.527
D-glucose
N593R, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
0.56
D-glucose
mutant enzyme Q448H, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C
0.58
D-glucose
immobilized enzyme, pH 6.5, 25°C
0.6
D-glucose
pH 7.3, 22°C, mutant enzyme E540K
0.6
D-glucose
pH 7.3, 22°C, mutant enzyme E540K with a C-terminal His6-tag
0.61
D-glucose
-
pH 6.5, 30°C
0.64
D-glucose
T169G/E542K/V546C, mutant, 30°C, substrate D-glucose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay
0.658
D-glucose
V546P/T169G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
0.69
D-glucose
mutant enzyme T169G, pH and temperature not specified in the publication
0.691
D-glucose
T169G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
0.698
D-glucose
wild-type, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
0.712
D-glucose
-
mutant enzyme F454A, at pH 6.5 and 30°C
0.72
D-glucose
mutant enzyme L545C, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C
0.74
D-glucose
-
at pH 6.5 and 25°C
0.749
D-glucose
L537W mutant, substrate D-glucose (O2 concentration constant), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.76
D-glucose
wild-type, mutant, 30°C, substrate D-glucose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay
0.76
D-glucose
wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
0.77
D-glucose
mutant enzyme H450G/E542K/V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C
0.773
D-glucose
R472G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
0.78
D-glucose
wild-type, mutant, 50°C, substrate D-glucose (1,4-benzoquinone saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay
0.8
D-glucose
pH 7.3, 22°C, recombinant wild-type enzyme
0.8 - 11
D-glucose
H548I, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
0.81
D-glucose
mutant enzyme N593C, with 1 ferrocenium ion as electron acceptor, at pH 6.5 and 30°C
0.82
D-glucose
-
oxygen concentration 0.25 mM
0.84
D-glucose
substrate D-glucose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
0.851
D-glucose
L537G mutant, substrate D-glucose (O2 concentration constant), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.851
D-glucose
-
wild type enzyme, at pH 6.5 and 30°C
0.88
D-glucose
H548R, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
0.886
D-glucose
R472L, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
0.9
D-glucose
-
mutant enzyme T169G, in 50 mM sodium phosphate (pH 7.0), at 4°C
0.93
D-glucose
native enzyme, pH 6.5, 25°C
0.939
D-glucose
wild-type, substrate D-glucose (O2 concentration constant), standard chromogenic ABTS assay (azino-bis-(3-ethylbenzthiazolin-6-sulfonic acid), horse-radish peroxidase, measuring absorbtion at 420 nm), pH 6.5, 30°C
0.939
D-glucose
wild type enzyme, pH and temperature not specified in the publication
0.948
D-glucose
-
wild type enzyme F454Y, at pH 6.5 and 30°C
0.952
D-glucose
V546C/T169N, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
0.98
D-glucose
mutant enzyme N593C, with O2 as electron acceptor, at pH 6.5 and 30°C
0.984
D-glucose
V546G/T169G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
0.987
D-glucose
mutant enzyme H450G, in 50 mM phosphate buffer (pH 6.5) at 60°C
0.987
D-glucose
mutant enzyme H450G, pH and temperature not specified in the publication
0.99
D-glucose
His-tagged recombinant wild type enzyme, in 50 mM phosphate buffer (pH 6.5) at 60°C
1.09
D-glucose
-
oxygen concentration 1.07 mM
1.1
D-glucose
pH 7.3, 22°C, wild-type enzyme
1.1
D-glucose
-
with O2 as electron acceptor, at pH 6.5 and 37°C
1.13
D-glucose
-
wild type enzyme, in 50 mM sodium phosphate (pH 7.0), at 4°C
1.15
D-glucose
T169G/E542K/V546C, mutant, 50°C, substrate D-glucose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay
1.18
D-glucose
wild-type, mutant, 50°C, substrate D-glucose (O2 saturated), pH 6.5, standard 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) assay
1.28
D-glucose
-
pH 7.0, 37°C
1.5
D-glucose
mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
1.5
D-glucose
mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
1.5 - 2
D-glucose
-
V546C/E542K mutant, substrate D-glucose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
1.51
D-glucose
E542K with a hexa-histidine tag, 0.1 M ethanolamine buffer at pH 10.5
1.6
D-glucose
mutant enzyme T166R, with ferrocenium ion as electron acceptor, at pH 6.5 and 30°C
1.7
D-glucose
-
mutant enzyme T169S, in 50 mM sodium phosphate (pH 7.0), at 4°C
1.7
D-glucose
mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
1.7
D-glucose
mutant enzyme L547R, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C
1.77
D-glucose
-
pH 6.5, 30°C, recombinant enzyme
1.8
D-glucose
mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
1.9
D-glucose
-
O2 concentration constant, pH 7.0, absorbance at 420 nm resulting from the oxidation of diammonium-2,2'-azinobis-(3-ethylbenzthiazolin-6-sulfonic acid) by H2O2
1.9
D-glucose
wild type enzyme, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C
2.1
D-glucose
mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
2.1
D-glucose
mutant enzyme T166R, with 2,6-dichlorophenolindophenol as electron acceptor, at pH 6.5 and 30°C
2.1
D-glucose
-
with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37°C
2.2
D-glucose
V546P, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
2.3
D-glucose
-
wild type enzyme, at pH 6.5, temperature not specified in the publication
2.43
D-glucose
mutant enzyme H450G/V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C
2.43
D-glucose
mutant enzyme H450G/V546C, pH and temperature not specified in the publication
2.5
D-glucose
mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
2.5
D-glucose
-
mutant enzyme N593C, at pH 6.5, temperature not specified in the publication
2.72
D-glucose
recombinant protein, 0.1 M PBS buffer at pH 8
2.86
D-glucose
-
pH 6.5, 30°C, recombinant enzyme
3.06
D-glucose
V546C, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
3.06
D-glucose
mutant enzyme V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C
3.06
D-glucose
mutant enzyme V546C, pH and temperature not specified in the publication
3.1
D-glucose
pH and temperature not specified in the publication
3.2
D-glucose
-
O2 concentration constant, pH 7.0, calculated with kinetic equation
4.6
D-glucose
-
mutant enzyme T169N, in 50 mM sodium phosphate (pH 7.0), at 4°C
5.7
D-glucose
V546G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
5.97
D-glucose
mutant enzyme H167A, pH and temperature not specified in the publication
28
D-glucose
Q448N, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
30
D-glucose
Q448S, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
30
D-glucose
-
mutant enzyme T169A, in 50 mM sodium phosphate (pH 7.0), at 4°C
45
D-glucose
-
pH 7.0, 25°C, reductive half-reaction, wild-type enzyme and mutant T169S
47
D-glucose
-
pH 7.0, 25°C, reductive half-reaction, mutant T169N
100
D-glucose
Q448C, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay
240
D-glucose
-
at pH 4.5
295
D-mannose

-
pH 7.0, 37°C
370
D-mannose
-
at pH 6.5 and 25°C
2 - 3
D-melibiose

mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
50
D-melibiose
mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
240
D-melibiose
mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
260
D-melibiose
mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
350
D-melibiose
mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
390
D-melibiose
mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
1500
D-melibiose
wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C
2.4
D-xylose

pH 7.3, 22°C, mutant enzyme K312E with a C-terminal His6-tag
6.21
D-xylose
100 mM ethanolamine buffer, pH 10.5
6.3
D-xylose
at 30°C, pH 6.5
6.3
D-xylose
immobilized enzyme, pH 6.5, 25°C
6.6
D-xylose
pH 7.3, 22°C, mutant enzyme E540K with a C-terminal His6-tag
7.9
D-xylose
pH 7.3, 22°C, mutant enzyme E540K
18.1
D-xylose
-
pH 6.5, 30°C, recombinant enzyme
18.4
D-xylose
native enzyme, pH 6.5, 25°C
20.9
D-xylose
substrate D-xylose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5
25
D-xylose
-
oxygen concentration 1.07 mM
25
D-xylose
pH 7.3, 22°C, recombinant wild-type enzyme