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EC Tree
The expected taxonomic range for this enzyme is: Gluconobacter oxydans
Synonyms
D-sorbitol dehydrogenase (NADP+), D-sorbitol:NADP+ oxidoreductase (L-sorbose-forming), SLDH,
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D-sorbitol dehydrogenase (NADP+)
D-sorbitol:NADP+ oxidoreductase (L-sorbose-forming)
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D-sorbitol dehydrogenase (NADP+)
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D-sorbitol dehydrogenase (NADP+)
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SLDH
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D-sorbitol + NADP+ = L-sorbose + NADPH + H+
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D-sorbitol:NADP+-oxidroreductase (L-sorbose-forming)
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D-arabinitol + NADP+
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D-sorbitol + NADP+
L-sorbose + NADPH + H+
mannitol + NADP+
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additional information
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D-sorbitol + NADP+
L-sorbose + NADPH + H+
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best substrate
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D-sorbitol + NADP+
L-sorbose + NADPH + H+
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best substrate
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additional information
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no activity is detected with L-arabinitol, xylitol, ribitol, myo-inositol, and glycerol
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additional information
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no activity is detected with L-arabinitol, xylitol, ribitol, myo-inositol, and glycerol
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NADP+
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the enzyme exhibits high preference for NADP+ (vs. 2.5% relative activity with NAD+)
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additional information
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Mg2+, Co2+, and Mn2+ ions show no stimulatory influence on enzyme activity
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D-sorbitol
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inhibitory at more than 10% (w/v)
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38.9
D-sorbitol
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at pH 10.0 and 25°C
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3820
D-sorbitol
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at pH 10.0 and 25°C
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98.1
D-sorbitol
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at pH 10.0 and 25°C
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25 - 75
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more than 40% activity between 25 and 75°C
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brenda
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brenda
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54000
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2 * 54000, SDS-PAGE
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homodimer
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2 * 54000, SDS-PAGE
homodimer
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2 * 54000, SDS-PAGE
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stability of the enzyme significantly improves (up to 13.6fold) after cross-linking of immobilized enzyme on silica nanoparticles and retains 62.8% residual activity after 10 cycles of reuse
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Ni-NTA column chromatography
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expressed in Escherichia coli BL21(DE3)-CodonPlus RIL cells
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Kim, T.S.; Patel, S.K.; Selvaraj, C.; Jung, W.S.; Pan, C.H.; Kang, Y.C.; Lee, J.K.
A highly efficient sorbitol dehydrogenase from Gluconobacter oxydans G624 and improvement of its stability through immobilization
Sci. Rep.
6
33438
2016
Gluconobacter oxydans, Gluconobacter oxydans G624
brenda
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1.1.1.B60 D-sorbitol dehydrogenase (NADP+)
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