BRENDA Home
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB CommentsThe enzyme, purified from the bacterium Thauera aromatica, is involved in an anaerobic toluene degradation pathway. It is specific for NAD+.
The expected taxonomic range for this enzyme is: Thauera aromatica
Reaction Schemes
(2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA
+
=
(S)-2-benzoylsuccinyl-CoA
+
+
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase
-
-
bbsCD
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA + NAD+ = (S)-2-benzoylsuccinyl-CoA + NADH + H+
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA:NAD+ oxidoreductase
The enzyme, purified from the bacterium Thauera aromatica, is involved in an anaerobic toluene degradation pathway. It is specific for NAD+.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(S)-2-benzoylsuccinyl-CoA + NADH + H+
(2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA + NAD+
-
-
-
-
?
additional information
?
-
-
the enzyme does not use NADP+ as electron acceptor
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(S)-2-benzoylsuccinyl-CoA + NADH + H+
(2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA + NAD+
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.096
(S)-2-benzoylsuccinyl-CoA
-
at pH 6.2 and 30°C
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.28
-
crude extract, at pH 6.2 and 30°C
0.86
-
after 3.1 enrichment, at pH 6.2 and 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
heterotetramer
-
2 * 26000 + 2 * 28000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
sitting drop vapor diffusion method, using 0.2 M Mg (CH3COO)2, 0.1 M sodium cacodylate, pH 6.5, and 20% (w/v) PEG 8000
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DEAE column chromatography and hydroxyapatite column chromatography
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli BL21(DE3) cells
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
von Horsten, S.; Lippert, M.L.; Geisselbrecht, Y.; Schuehle, K.; Schall, I.; Essen, L.O.; Heider, J.
Inactive pseudoenzyme subunits in heterotetrameric BbsCD, a novel short-chain alcohol dehydrogenase involved in anaerobic toluene degradation
FEBS J.
289
1023-1042
2022
Thauera aromatica
brenda
Select items on the left to see more content.
html completed
1.1.1.429 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase
more
top
