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IUBMB CommentsThe enzyme, purified from the bacterium Thauera aromatica, is involved in an anaerobic toluene degradation pathway. It is specific for NAD+.
The expected taxonomic range for this enzyme is: Thauera aromatica
Reaction Schemes
(2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA
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(S)-2-benzoylsuccinyl-CoA
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(S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase
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bbsCD

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(2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA + NAD+ = (S)-2-benzoylsuccinyl-CoA + NADH + H+
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(2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA:NAD+ oxidoreductase
The enzyme, purified from the bacterium Thauera aromatica, is involved in an anaerobic toluene degradation pathway. It is specific for NAD+.
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(S)-2-benzoylsuccinyl-CoA + NADH + H+
(2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA + NAD+
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additional information
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the enzyme does not use NADP+ as electron acceptor
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(S)-2-benzoylsuccinyl-CoA + NADH + H+
(2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA + NAD+
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0.096
(S)-2-benzoylsuccinyl-CoA
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at pH 6.2 and 30°C
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0.28
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crude extract, at pH 6.2 and 30°C
0.86
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after 3.1 enrichment, at pH 6.2 and 30°C
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brenda
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heterotetramer
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2 * 26000 + 2 * 28000, SDS-PAGE
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sitting drop vapor diffusion method, using 0.2 M Mg (CH3COO)2, 0.1 M sodium cacodylate, pH 6.5, and 20% (w/v) PEG 8000
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DEAE column chromatography and hydroxyapatite column chromatography
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expressed in Escherichia coli BL21(DE3) cells
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von Horsten, S.; Lippert, M.L.; Geisselbrecht, Y.; Schuehle, K.; Schall, I.; Essen, L.O.; Heider, J.
Inactive pseudoenzyme subunits in heterotetrameric BbsCD, a novel short-chain alcohol dehydrogenase involved in anaerobic toluene degradation
FEBS J.
289
1023-1042
2022
Thauera aromatica
brenda
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