Information on EC 1.1.1.415 - noscapine synthase

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The expected taxonomic range for this enzyme is: Papaver somniferum

EC NUMBER
COMMENTARY hide
1.1.1.415
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RECOMMENDED NAME
GeneOntology No.
noscapine synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
narcotine hemiacetal + NAD+ = noscapine + NADH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
noscapine biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
narcotine hemiacetal:NAD+ 1-oxidoreductase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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suppression of NOS transcript levels in opium poppy plants subjected to virus-induced gene silencing results in a corresponding reduction in the accumulation of noscapine and an increase in narcotinehemiacetal levels in the latex
metabolism
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the enzyme catalyses the last step in the biosynthesis of the isoquinoline alkaloid noscapine
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
narcotine hemiacetal + NAD+
noscapine + NADH + H+
show the reaction diagram
narcotine hemiacetal + NADP+
noscapine + NADPH + H+
show the reaction diagram
the enzyme exhibits higher catalytic efficiency with NAD+ as the cofactor compared with NADP+
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-
r
noscapine + NADH + H+
narcotine hemiacetal + NAD+
show the reaction diagram
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-
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r
noscapine + NADPH + H+
narcotine hemiacetal + NADP+
show the reaction diagram
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-
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r
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
narcotine hemiacetal + NAD+
noscapine + NADH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
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the enzyme exhibits higher catalytic efficiency with NAD+ as the cofactor compared with NADP+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0331
NAD+
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pH 8.0, 30°C
1.249
NADP+
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pH 8.0, 30°C
0.0209 - 0.268
narcotine hemiacetal
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.54
NAD+
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pH 8.0, 30°C
1.73
NADP+
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pH 8.0, 30°C
0.59 - 1.69
narcotine hemiacetal
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
16.24
NAD+
-
pH 8.0, 30°C
1.38
NADP+
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pH 8.0, 30°C
6.296 - 28.097
narcotine hemiacetal
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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intermediate level
Manually annotated by BRENDA team
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intermediate level
Manually annotated by BRENDA team
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least abundant in roots
Manually annotated by BRENDA team
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NOS transcripts are detected in all opium poppy organs, but are most abundant in stems
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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Saccharomyces cerevisiae CSY1060 and CSY1061 strains are used as the base strain for construction of the de novo noscapine biosynthetic strains
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
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complete biosynthesis of noscapine and halogenated alkaloids in yeast and optimizing noscapine production toward scalable manufacturing. Engineered strain contains 25 heterologous plant, bacteria, and mammalian genes and 6 mutant or overexpressed yeast genes. The noscapine biosynthetic pathway incorporates seven endomembrane-localized plant enzymes, highlighting the ability of the yeast to functionally express and properly localize large numbers of heterologous enzymes into the endoplasm reticulum. Noscapine titers were improved by 18000fold (to low mg/l levels) via a combination of enzyme engineering, pathway and strain engineering, and fermentation optimization. Microbial fermentation can be used to produce halogenated alkaloid derivatives, which can ultimately serve as potential drug leads, through feeding amino acid derivatives to strains