We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments This enzyme differs from EC 1.1.1.187, GDP-4-dehydro-D-rhamnose reductase, in that the only product formed is GDP-alpha-D-rhamnose. D-Rhamnose is a constituent of lipopolysaccharides of Gram-negative plant and human pathogenic bacteria.
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
gdp-6-deoxy-d-lyxo-4-hexulose reductase,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GDP-4-keto-6-deoxy-D-mannose reductase
-
-
-
GDP-4-keto-6-deoxy-D-mannose reductase (ambiguous)
GDP-6-deoxy-D-lyxo-4-hexulose reductase
GDP-6-deoxy-D-mannose reductase
-
GDP-4-keto-6-deoxy-D-mannose reductase (ambiguous)
-
GDP-4-keto-6-deoxy-D-mannose reductase (ambiguous)
-
GDP-6-deoxy-D-lyxo-4-hexulose reductase
-
-
-
GDP-6-deoxy-D-lyxo-4-hexulose reductase
-
GDP-6-deoxy-D-lyxo-4-hexulose reductase
-
RMD
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GDP-alpha-D-rhamnose + NAD(P)+ = GDP-4-dehydro-alpha-D-rhamnose + NAD(P)H + H+
GDP-alpha-D-rhamnose + NAD(P)+ = GDP-4-dehydro-alpha-D-rhamnose + NAD(P)H + H+
-
-
-
-
GDP-alpha-D-rhamnose + NAD(P)+ = GDP-4-dehydro-alpha-D-rhamnose + NAD(P)H + H+
This enzyme differs from EC 1.1.1.187, GDP-4-dehydro-D-rhamnose reductase, in that the only product formed is GDP-D-rhamnose (GDP-6-deoxy-D-mannose). D-Rhamnose is a constituent of lipopolysaccharides of Gram-negative plant and human pathogenic bacteria
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
oxidation
-
-
-
redox reaction
-
-
-
reduction
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GDP-6-deoxy-alpha-D-mannose:NAD(P)+ 4-oxidoreductase (D-rhamnose-forming)
This enzyme differs from EC 1.1.1.187, GDP-4-dehydro-D-rhamnose reductase, in that the only product formed is GDP-alpha-D-rhamnose. D-Rhamnose is a constituent of lipopolysaccharides of Gram-negative plant and human pathogenic bacteria.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H + H+
GDP-D-rhamnose + NAD(P)+
-
-
?
GDP-4-dehydro-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-D-mannose + NADP+
-
-
-
?
GDP-6-deoxy-D-lyxo-4-hexulose + NADH + H+
GDP-alpha-D-rhamnose + NAD+
GDP-6-deoxy-D-lyxo-4-hexulose + NADPH + H+
GDP-alpha-D-rhamnose + NADP+
GDP-6-deoxy-D-mannose + NAD(P)+
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
GDP-6-deoxy-D-lyxo-4-hexulose + NADH + H+
GDP-alpha-D-rhamnose + NAD+
-
-
ir
GDP-6-deoxy-D-lyxo-4-hexulose + NADH + H+
GDP-alpha-D-rhamnose + NAD+
-
-
ir
GDP-6-deoxy-D-lyxo-4-hexulose + NADH + H+
GDP-alpha-D-rhamnose + NAD+
-
-
-
ir
GDP-6-deoxy-D-lyxo-4-hexulose + NADPH + H+
GDP-alpha-D-rhamnose + NADP+
-
-
ir
GDP-6-deoxy-D-lyxo-4-hexulose + NADPH + H+
GDP-alpha-D-rhamnose + NADP+
-
-
ir
GDP-6-deoxy-D-lyxo-4-hexulose + NADPH + H+
GDP-alpha-D-rhamnose + NADP+
-
-
-
ir
GDP-6-deoxy-D-mannose + NAD(P)+
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
-
-
?
GDP-6-deoxy-D-mannose + NAD(P)+
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H + H+
GDP-D-rhamnose + NAD(P)+
-
-
-
?
GDP-4-dehydro-6-deoxy-D-mannose + NADPH
GDP-6-deoxy-D-mannose + NADP+
-
-
-
?
GDP-6-deoxy-D-mannose + NAD(P)+
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
GDP-6-deoxy-D-mannose + NAD(P)+
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
-
-
-
?
GDP-6-deoxy-D-mannose + NAD(P)+
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NAD+
-
-
NADH
-
-
NADP+
-
-
NADPH
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
L420-91T
GenBank
brenda
no activity in Schistosoma mansoni
-
-
-
brenda
-
-
-
brenda
L420-91T
GenBank
brenda
-
-
-
brenda
-
UniProt
brenda
expression in Saccharomyces cerevisiae
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
metabolism
the enzyme consumes the immediate precursor to GDP-L-fucose to produce GDP-D-rhamnose, which might be a dead-end product in recombinant insect cells
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
33900
-
x * 34000, SDS-PAGE of FLAG-tagged recombinant enzyme, x * 33900, calculated
38100
x * 38100, SDS-PAGE
34000
-
x * 34000, recombinant FLAG-tagged enzyme
34000
-
x * 34000, SDS-PAGE of FLAG-tagged recombinant enzyme, x * 33900, calculated
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
x * 38100, SDS-PAGE
?
-
x * 34000, recombinant FLAG-tagged enzyme
?
-
x * 34000, SDS-PAGE of FLAG-tagged recombinant enzyme, x * 33900, calculated
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
GDP-L-fucose biosynthesis in eukaryotic cells is reduced by expression of bacterial Rmd, which converts GDP-4-dehydro-6-deoxy-D-mannose to GDP-D-rhamnose, assessing a vector-based glycoengineering strategy for blocking glycoprotein fucosylation in insect cells, overview. N-glycan profiling by MALDI-TOF mass spectrometry
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expression in Saccharomyces cerevisiae
-
gene rmd, constitutive recombinant overexpression in Spodoptera frugiperda Sf9 cells or in Trichoplusia ni High Five cells under the transcriptional control of the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) immediate early 1 (ie1) promoter and via baculovirus transfection. Rmd to blocks the production of GDP-L-fucose and glycoprotein fucosylation because GDP-L-fucose is required as the donor substrate for this process
overexpression in Escherichia coli
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
synthesis
-
generating GDP-rhamnose for in vitro rhamnosylation of glycoproteins and glycopeptides
synthesis
-
the functional expression of the Pseudomonas aeruginosa enzyme in Saccharomyces cerevisiae will provide a tool for generating GDP-rhamnose for in vitro rhamnosylation of glycoprotein and glycopeptides
synthesis
-
generation of GDP-rhamnose by coexpression of enzyme with GDP-mannose-4,6-dehydratase in Saccharomyces cerevisiae
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Kneidinger, B.; Graninger, M.; Adam, G.; Puchberger, M.; Kosma, P.; Zayni, S.; Messner, P.
Identification of two GDP-6-deoxy-D-lyxo-4-hexulose reductases synthesizing GDP-D-rhamnose in Aneurinibacillus thermoaerophilus L420-91T
J. Biol. Chem.
276
5577-5583
2001
Aneurinibacillus thermoaerophilus, Aneurinibacillus thermoaerophilus (Q6T1X6), Aneurinibacillus thermoaerophilus L420-91T (Q6T1X6), Aneurinibacillus thermoaerophilus L420-91T
brenda
Mäki, M.; Järvinen, N.; Räbinä, J.; Roos, C.; Maaheimo, H.; Mattila, P.; Renkonen, R.
Functional expression of Pseudomonas aeruginosa GDP-4-keto-6-deoxy-D-mannose reductase which synthesizes GDP-rhamnose
Eur. J. Biochem.
269
593-601
2002
Pseudomonas aeruginosa
brenda
Chin, Y.W.; Park, J.B.; Park, Y.C.; Kim, K.H.; Seo, J.H.
Metabolic engineering of Corynebacterium glutamicum to produce GDP-L-fucose from glucose and mannose
Bioprocess Biosyst. Eng.
36
749-756
2013
Pseudomonas aeruginosa (Q9HTB6)
brenda
Peterson, N.; Anderson, T.; Wu, X.; Yoshino, T.
In silico analysis of the fucosylation-associated genome of the human blood fluke Schistosoma mansoni: cloning and characterization of the enzymes involved in GDP-L-fucose synthesis and Golgi import
Parasit. Vectors
6
201
2013
no activity in Schistosoma mansoni
brenda
Select items on the left to see more content.
html completed
HOME
login
history
all enzymes
BRENDA home
History
1.1.1.281 GDP-4-dehydro-6-deoxy-D-mannose reductase
more
top
Information
