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Information on EC 1.1.1.200 - aldose-6-phosphate reductase (NADPH)
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EC Tree
1.1.1.200 aldose-6-phosphate reductase (NADPH)IUBMB Comments
In the reverse reaction, acts also on D-galactose 6-phosphate and, more slowly, on D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate.
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Word Map
- 1.1.1.200
- sorbitol
- apple
- domestica
- malus
- starch
- rosaceae
- peach
- adp-glucose
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borkh
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2.4.1.14
- hexokinase
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sink
- invertase
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greensleeves
- fructokinase
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pyrophosphorylase
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prunus
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2.7.7.27
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gala
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sugar-phosphates
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aldo-keto
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sink-source
- glucitol
- persica
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mixed-type
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
aldose-6-phosphate reductase, a6prase, ald6prase, nadph-dependent aldose 6-phosphate reductase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
A6PR
alditol 6-phosphate:NADP 1-oxidoreductase
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aldose-6-P reductase
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aldose-6-phosphate reductase
aldose-6-phosphate reductase [NADPH]
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NADP-S6PDH
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NADPH-dependent aldose 6-phosphate reductase
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reductase, aldose 6-phosphate
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S6PDH
A6PR
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aldose-6-phosphate reductase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
D-sorbitol 6-phosphate + NADP+ = D-glucose 6-phosphate + NADPH + H+
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
D-aldose-6-phosphate:NADP+ 1-oxidoreductase
In the reverse reaction, acts also on D-galactose 6-phosphate and, more slowly, on D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate.
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CAS REGISTRY NUMBER
COMMENTARY
76901-04-7
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-galactose 6-phosphate + NADPH
?
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reduced at a higher rate than D-glucose 6-phosphate
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r
D-glucose 6-phosphate + NADPH + H+
D-glucitol-6-phosphate + NADP+
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r
D-glucose 6-phosphate + NADPH + H+
D-sorbitol 6-phosphate + NADP+
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?
D-glucose 6-phosphate + NADPH + H+
D-sorbitol 6-phosphate + NADP+
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r
D-glucose 6-phosphate + NADPH + H+
D-sorbitol 6-phosphate + NADP+
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?
D-sorbitol 6-phosphate + NADP+
D-glucose 6-phosphate + NADPH + H+
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r
D-sorbitol 6-phosphate + NADP+
D-glucose 6-phosphate + NADPH + H+
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r
D-sorbitol 6-phosphate + NADP+
D-glucose 6-phosphate + NADPH + H+
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?
additional information
?
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not: D-glucose 1-phosphate, D-fructose 6-phosphate, D-ribose 5-phosphate, D-glucose, sorbitol
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additional information
?
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when sorbitol synthesis is decreased by antisense inhibition of A6PR expression, cytosolic FBPase activity in vivo is down-regulated to reduce the carbon flux to cytosol and starch synthesis in the chloroplast is consequently upregulated
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?
additional information
?
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effects of mild and severe water stress on the sorbitol metabolism in leaves, A6PR activity is increased in response to water stress, while sorbitol accumulates under mild to moderate stress but is decreased under severe stress in leaves, overview
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?
additional information
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both, the N- and the C-term tagged enzymes exhibit similar affinity toward substrates
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?
additional information
?
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both, the N- and the C-term tagged enzymes exhibit similar affinity toward substrates
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?
additional information
?
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effects of fruit removal on the carbon metabolism in leaves, the enzyme activity ins increased after fruit removal, although starch and sorbitol accumulate in the leaves, overview
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?
additional information
?
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almost no activity with mannose 6-phosphate or ribose 5-phosphate, no activity with NADH and NAD+
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additional information
?
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almost no activity with mannose 6-phosphate or ribose 5-phosphate, no activity with NADH and NAD+
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-glucose 6-phosphate + NADPH + H+
D-sorbitol 6-phosphate + NADP+
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?
D-glucose 6-phosphate + NADPH + H+
D-sorbitol 6-phosphate + NADP+
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r
D-glucose 6-phosphate + NADPH + H+
D-sorbitol 6-phosphate + NADP+
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?
D-sorbitol 6-phosphate + NADP+
D-glucose 6-phosphate + NADPH + H+
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r
D-sorbitol 6-phosphate + NADP+
D-glucose 6-phosphate + NADPH + H+
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?
additional information
?
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when sorbitol synthesis is decreased by antisense inhibition of A6PR expression, cytosolic FBPase activity in vivo is down-regulated to reduce the carbon flux to cytosol and starch synthesis in the chloroplast is consequently upregulated
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?
additional information
?
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effects of mild and severe water stress on the sorbitol metabolism in leaves, A6PR activity is increased in response to water stress, while sorbitol accumulates under mild to moderate stress but is decreased under severe stress in leaves, overview
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?
additional information
?
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effects of fruit removal on the carbon metabolism in leaves, the enzyme activity ins increased after fruit removal, although starch and sorbitol accumulate in the leaves, overview
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?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
no significant effects are observed when the activity of the enzyme is assayed in the presence of 5 mM Mg2+ or Ca2+
additional information
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no significant effects are observed when the activity of the enzyme is assayed in the presence of 5 mM Mg2+ or Ca2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
cysteine
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47% inhibition of oxidation and 11% inhibition of reduction at 10 mM
Diamide
enzymatic activity decreases to approximately 7% at 500 mM diamide
H2O2
H2O2 oxidation causes enzyme inactivation in a time- and concentration-dependent manner
iodoacetate
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42% inhibition of oxidation and 19% inhibition of reduction at 10 mM
Na2EDTA
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35% inhibition of oxidation and 11% inhibition of reduction at 10 mM
ZnSO4
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complete inhibition of oxidation and 80% inhibition of reduction at 1.25 mM
additional information
no significant differences in activity when ATP, ADP, AMP, ADP-glucose, UTP, UDP-glucose, inorganic phosphate or diphosphate (5 mM each), 10% (v/v) glycerol, 500 mM glucitol or 20% (w/v) sucrose are added to the enzyme assay mixture
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additional information
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no significant differences in activity when ATP, ADP, AMP, ADP-glucose, UTP, UDP-glucose, inorganic phosphate or diphosphate (5 mM each), 10% (v/v) glycerol, 500 mM glucitol or 20% (w/v) sucrose are added to the enzyme assay mixture
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additional information
no inhibition by D-trehalose 6-phosphate, D-fructose 2,6-bisphosphate, D-xylitol, D-glucose and D-fructose. Not affected by 5 mM GSSG
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additional information
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no inhibition by D-trehalose 6-phosphate, D-fructose 2,6-bisphosphate, D-xylitol, D-glucose and D-fructose. Not affected by 5 mM GSSG
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
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enzyme activity is rapidly induced by water stress
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 10
N- and the C-term tagged enzymes. Both recombinant enzymes similarly show optimal activity in a broad pH range in the direction of D-glucose 6-phosphate reduction, and a narrow range (pH 9.0-10.0) in the opposite oxidative direction
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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from a 1-year-old shoot, and from limbs carrying fruits or with fruits removed, the leaves of the latter show increased enzyme activity
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
level of soluble recombinant enzyme is much higher in the N-term tagged protein than in the C-term tagged one. In the C-term tagged one the majority of the protein is in the insoluble form
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brenda
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
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sorbitol-6-phosphate dehydrogenase is a key enzyme for sorbitol synthesis and plays an important role in the alleviation of salinity stress in plants
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