Information on EC 1.1.1.20 - glucuronolactone reductase

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The expected taxonomic range for this enzyme is: Rattus norvegicus

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EC NUMBER
COMMENTARY hide
1.1.1.20
-
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RECOMMENDED NAME
GeneOntology No.
glucuronolactone reductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-gulono-1,4-lactone + NADP+ = D-glucurono-3,6-lactone + NADPH + H+
show the reaction diagram
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
reduction
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Ascorbate and aldarate metabolism
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-
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SYSTEMATIC NAME
IUBMB Comments
L-gulono-1,4-lactone:NADP+ 1-oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
9028-30-2
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-galacturonic acid + NADPH
?
show the reaction diagram
-
-
-
-
?
D-glucuronic acid + NADPH
L-gulonic acid + NADP+
show the reaction diagram
-
-
-
-
?
D-glucurono-3,6-lactone + NADPH
D-gulono-1,4-lactone + NADP+
show the reaction diagram
-
oxidation activity is very low
-
-
r
D-iduronic acid + NADPH
?
show the reaction diagram
-
-
-
-
?
erythrose + NADPH
glycerol + NADP+
show the reaction diagram
-
-
-
-
?
glyceraldehyde + NADPH
?
show the reaction diagram
-
-
-
-
?
L-gulono-1,4-lactone + NADP+
D-glucurono-3,6-lactone + NADPH
show the reaction diagram
-
-
-
-
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-gulono-1,4-lactone + NADP+
D-glucurono-3,6-lactone + NADPH
show the reaction diagram
-
-
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
NADPH is 145-times as effective as NADH
NADPH
-
NADPH is 145-times as effective as NADH
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Barbital
-
-
Barbiturates
-
-
-
dithiothreitol
-
-
iodoacetic acid
-
-
Phenobarbital
-
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4
D-galacturonic acid
-
-
6
D-glucuronic acid
-
L-iduronic acid
9
D-glucuronolactone
-
-
0.045
NADPH
-
with D-glucuronic acid
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
13450 U/min * mg, 1 unit is defined as change of absorbance of 0.01/min at 340 nm
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 7.5
-
DL-glyceraldehyde
6 - 7
-
D-glucuronic acid
9.5
-
broad, oxidation of glycerol and L-gulonolactone
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
-
1 * 40000, SDS-PAGE
41000
-
gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 40000, SDS-PAGE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
180 min, 50% loss of activity; 30 min, 20% loss of activity; 60 min, 30% loss of activity
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing and thawing: 3 times causes 25% decrease of activity, 5 times causes 40% decrease of activity
-
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, 10 days, 30% loss of activity
-
0°C, 2 months, less than 10% loss of activity
-
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 1.1.1.20)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)