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EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
aminodioxyphosphoribosylaminopyrimidine reductase, HTP reductase, Rib7, RibD, RibG, riboflavin biosynthesis protein,
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aminodioxyphosphoribosylaminopyrimidine reductase
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riboflavin biosynthesis protein
RibD
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RibG
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riboflavin biosynthesis protein
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riboflavin biosynthesis protein
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5-amino-6-(5-phospho-D-ribitylamino)uracil + NADP+ = 5-amino-6-(5-phospho-D-ribosylamino)uracil + NADPH + H+
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5-amino-6-(5-phospho-D-ribitylamino)uracil:NADP+ 1'-oxidoreductase
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5-amino-2,6-dioxy-4-(5'-phosphoribosylamino)pyrimidine + NADPH
5-amino-2,6-dioxy-4-(5'-phosphoribitylamino)pyrimidine + NADP+
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
additional information
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5-amino-2,6-dioxy-4-(5'-phosphoribosylamino)pyrimidine + NADPH
5-amino-2,6-dioxy-4-(5'-phosphoribitylamino)pyrimidine + NADP+
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intermediate in the biosynthesis of riboflavin
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5-amino-2,6-dioxy-4-(5'-phosphoribosylamino)pyrimidine + NADPH
5-amino-2,6-dioxy-4-(5'-phosphoribitylamino)pyrimidine + NADP+
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intermediate in the biosynthesis of riboflavin
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5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
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5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
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5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
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5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
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additional information
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the enzyme is involved in riboflavin biosynthesis, overview
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additional information
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the enzyme is involved in riboflavin biosynthesis, overview
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additional information
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the enzyme is involved in riboflavin biosynthesis, overview
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5-amino-2,6-dioxy-4-(5'-phosphoribosylamino)pyrimidine + NADPH
5-amino-2,6-dioxy-4-(5'-phosphoribitylamino)pyrimidine + NADP+
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
additional information
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5-amino-2,6-dioxy-4-(5'-phosphoribosylamino)pyrimidine + NADPH
5-amino-2,6-dioxy-4-(5'-phosphoribitylamino)pyrimidine + NADP+
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intermediate in the biosynthesis of riboflavin
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5-amino-2,6-dioxy-4-(5'-phosphoribosylamino)pyrimidine + NADPH
5-amino-2,6-dioxy-4-(5'-phosphoribitylamino)pyrimidine + NADP+
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intermediate in the biosynthesis of riboflavin
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5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
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5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
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5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
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5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
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additional information
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the enzyme is involved in riboflavin biosynthesis, overview
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additional information
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the enzyme is involved in riboflavin biosynthesis, overview
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additional information
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the enzyme is involved in riboflavin biosynthesis, overview
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NADH
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NADH
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30% as effective as NADPH
NADPH
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Mg2+
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8
assay at
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37
assay at
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putative
UniProt
brenda
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brenda
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brenda
bifunctional enzyme; gene ribG
UniProt
brenda
bifunctional enzyme; gene ribG
UniProt
brenda
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brenda
gene ribG or ribD
UniProt
brenda
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brenda
gene ribD or ribG
UniProt
brenda
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brenda
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brenda
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22000
x * 22000, recombinant enzyme, SDS-PAGE
40000
2 * 42038, calculated, 2 * 40000, SDS-PAGE, tagged recombinant protein
42038
2 * 42038, calculated, 2 * 40000, SDS-PAGE, tagged recombinant protein
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x * 22000, recombinant enzyme, SDS-PAGE
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x * 22000, recombinant enzyme, SDS-PAGE
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dimer
2 * 42038, calculated, 2 * 40000, SDS-PAGE, tagged recombinant protein
dimer
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2 * 42038, calculated, 2 * 40000, SDS-PAGE, tagged recombinant protein
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additional information
domain structure, overview
additional information
domain structure, overview
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orthorhombic and tetragonal crystal forms at 2.2 and 2.0 A resolution
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D33A
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the mutant shows 0.1-0.6% activity compared to the wild type enzyme
D57N
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the mutant shows 0.1-0.6% activity compared to the wild type enzyme
N9A
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the mutant shows 0.1-0.6% activity compared to the wild type enzyme
R91A
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the mutation eliminates the endogenous NADPH binding and switches preference of the enzyme to NADH
S29A
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the mutant shows 0.1-0.6% activity compared to the wild type enzyme
S88E
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the mutation eliminates the endogenous NADPH binding and switches preference of the enzyme to NADH
additional information
a ribG mutant can be rescued by expression of ribB, overview
additional information
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a ribG mutant can be rescued by expression of ribB, overview
additional information
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a ribG mutant can be rescued by expression of ribB, overview
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-6°C, 50 mM Tris-HCl, pH 8.0, 1 month
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recombinant enzyme from Escherichia coli to homogeneity
recombinant enzyme from Escherichia coli to homogeneity
recombinant enzyme from Escherichia coli to homogeneity
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gene ribD, i.e. ribG, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
gene ribG, i.e. ribD, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
gene ribG, ribG forms an expression unit with ribY, a monofunctional GTP cyclohydrolase II, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3)
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Richter, G.; Fischer, M.; Krieger, C.; Eberhardt, S.; Luttgen, H.; Gerstenschläger, I.; Bacher, A.
Biosynthesis of riboflavin: characterization of the bifunctional deaminase-reductase of Escherichia coli and Bacillus subtilis
J. Bacteriol.
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2022-2028
1997
Bacillus subtilis (P17618), Escherichia coli (P25539)
brenda
Burrows, R.B.; Brown, G.M.
Presence in Escherichia coli of a deaminase and a reductase involved in biosynthesis of riboflavin
J. Bacteriol.
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657-667
1978
Escherichia coli, Escherichia coli B / ATCC 11303
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Grill, S.; Yamaguchi, H.; Wagner, H.; Zwahlen, L.; Kusch, U.; Mack, M.
Identification and characterization of two Streptomyces davawensis riboflavin biosynthesis gene clusters
Arch. Microbiol.
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377-387
2007
Streptomyces davaonensis (Q2VCF5), Streptomyces davaonensis, Streptomyces davaonensis 768 (Q2VCF5)
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Dawson, A.; Trumper, P.; Chrysostomou, G.; Hunter, W.N.
Structure of diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase from Acinetobacter baumannii
Acta Crystallogr. Sect. F
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611-617
2013
Acinetobacter baumannii (D0CB74)
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Chen, S.; Yen, T.; Chang, T.; Liaw, S.
Evolution of archaeal Rib7 and eubacterial RibG reductases in riboflavin biosynthesis Substrate specificity and cofactor preference
Biochem. Biophys. Res. Commun.
503
195-201
2018
Bacillus subtilis, Methanosarcina mazei
brenda
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