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Information on EC 1.1.1.175 - D-xylose 1-dehydrogenase
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1.1.1.175 D-xylose 1-dehydrogenaseSpecify your search results
Word Map
- 1.1.1.175
- d-xylonate
- nadp+
- crescentus
-
caulobacter
- d-glucose
-
nadp+-dependent
- reesei
-
trichoderma
- pentose
-
glucose-fructose
- xylitol
-
dehydratase
- l-arabinose
-
titre
- xylulose
-
azospirillum
-
xylonic
- alpha-ketoglutarate
-
non-food
- brasiliense
-
gluconobacter
- volcanii
- d-ribose
-
haloarcula
- lactonase
-
dihydrodiol
- oxydans
- marismortui
-
haloferax
-
kluyveromyces
- synthesis
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
(NAD)-linked D-xylose dehydrogenase, D-xylose 1-dehydrogenase, D-xylose dehydrogenase, More, NAD+-dependent xylose dehydrogenase, NAD-D-xylose dehydrogenase, XDH, XylB, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
(NAD)-linked D-xylose dehydrogenase
-
-
-
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D-xylose 1-dehydrogenase
D-xylose dehydrogenase
-
-
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NAD+-dependent xylose dehydrogenase
NAD-D-xylose dehydrogenase
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-
-
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XDH
XylB
additional information
additional information
-
the enzyme belongs to the short-chain dehydrogenase/reductase family
additional information
-
the enzyme belongs to the short-chain dehydrogenase/reductase family
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
D-xylose + NAD+ = D-xylonolactone + NADH + H+
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
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-
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redox reaction
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-
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reduction
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-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
MetaCyc
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SYSTEMATIC NAME
IUBMB Comments
D-xylose:NAD+ 1-oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY
62931-20-8
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-xylose + NAD+
?
79% of the activity with D-glucose and NADP+. The enzyme shows also activity with D-xylose and NAD+ as cofactor, D-glucose (NADP+ or NAD+ as cofactor), D-fucose (cofactor NADP+) and D-galactose (cofactor NADP+)
-
-
?
D-xylose + NAD+
?
79% of the activity with D-glucose and NADP+. The enzyme shows also activity with D-xylose and NAD+ as cofactor, D-glucose (NADP+ or NAD+ as cofactor), D-fucose (cofactor NADP+) and D-galactose (cofactor NADP+)
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-
?
D-xylose + NAD+
D-xylono-1,5-lactone + NADH + H+
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the enzyme is involved in the pathway for D-xylose metabolism, overview
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-
r
D-xylose + NAD+
D-xylono-1,5-lactone + NADH + H+
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the enzyme strongly prefers D-xylose as a substrate, overview
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-
r
D-xylose + NAD+
D-xylono-1,5-lactone + NADH + H+
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the enzyme is involved in the pathway for D-xylose metabolism, overview
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r
D-xylose + NAD+
D-xylono-1,5-lactone + NADH + H+
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the enzyme strongly prefers D-xylose as a substrate, overview
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-
r
D-xylose + NAD+
D-xylonolactone + NADH + H+
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highly specific for both substrates
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?
D-xylose + NAD+
D-xylonolactone + NADH + H+
-
highly specific for both substrates
-
?
D-xylose + NAD+
D-xylonolactone + NADH + H+
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highly specific for both substrates
-
?
D-xylose + NAD+
D-xylonolactone + NADH + H+
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highly specific for both substrates
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?
D-xylose + NAD+
D-xylonolactone + NADH + H+
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specific hydrogen transfer for pro-R -A site
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?
additional information
?
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L-arabinose is a poor substrate
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-
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-xylose + NAD+
D-xylono-1,5-lactone + NADH + H+
-
the enzyme is involved in the pathway for D-xylose metabolism, overview
-
-
r
D-xylose + NAD+
D-xylono-1,5-lactone + NADH + H+
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the enzyme is involved in the pathway for D-xylose metabolism, overview
-
-
r
D-xylose + NAD+
D-xylonolactone + NADH + H+
-
highly specific for both substrates
-
?
D-xylose + NAD+
D-xylonolactone + NADH + H+
-
highly specific for both substrates
-
?
D-xylose + NAD+
D-xylonolactone + NADH + H+
-
highly specific for both substrates
-
?
D-xylose + NAD+
D-xylonolactone + NADH + H+
-
highly specific for both substrates
-
?
D-xylose + NAD+
D-xylonolactone + NADH + H+
-
specific hydrogen transfer for pro-R -A site
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene xylB, the enzyme is encoded in the xylose-inducible xylXABCD operon
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brenda
gene xylB, the enzyme is encoded in the xylose-inducible xylXABCD operon
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brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
inducible enzyme synthesis by growth in D-xylose-containing medium
brenda
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inducible enzyme synthesis by growth in D-xylose-containing medium
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brenda
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inducible enzyme synthesis by growth in D-xylose-containing medium
brenda
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inducible enzyme synthesis by growth in D-xylose-containing medium
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brenda
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
XDH_CAUVN
Caulobacter vibrioides (strain NA1000 / CB15N)
248
0
26641
Swiss-Prot
B8MH03_TALSN
Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006)
320
0
36056
TrEMBL
A0A2Z2NPR7_9GAMM
252
1
27080
TrEMBL
A0A0L0MBF5_9BURK
284
0
30844
TrEMBL
A0A292ZCV1_SPHSA
Sphingobium fuliginis (strain ATCC 27551)
252
0
27087
TrEMBL
A0A2S6SD04_9PROT
256
0
29017
TrEMBL
Q1QWS7_CHRSD
Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768)
245
0
26688
TrEMBL
GLCDH_HALMT
Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4)
357
0
39242
Swiss-Prot
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
both D-xylono-gamma-lactone and D-xylonate are produced when the Caulobacter crescentus gene xylB encoding D-xylose dehydrogenase is expressed in Saccharomyces cerevisiae, with or without co-expressionof xylC (D-xylonolactonase). XylC facilitates rapid opening of the lactone and more D-xylonate is initially produced than in its absence. In vivo [1H]NMR analysis of cell extracts, culture media and intact cells is used for analysis. The lactone and linear forms of D-xylonic acid are produced, accumulated intracellularly, and partially exported within 1560 min after D-xylose provision. Co-expression of xylB and xylC leads to rapid loss of pHluorin fluorescence and loss of vitality during production of D-xylonate. Loss of vitality in the presence of D-xylose is correlated to the extracellular pH, but fluorescence is lost from xylB and xylC expressing cells regardless of the extracellular condition. Method optimization, overview
additional information
-
engineering of non-conventional yeast Pichia kudriavzevii strain VTT C-79090T to recombinantly express the D-xylose dehydrogenase coding gene from Caulobacter crescentus. With this single modification the recombinant strain VTT C-79090T produces up to 171 g/l of D-xylonate from 171 g/l D-xylose at a rate of 1.4 g/l/h and a high yield, which is comparable with D-xylonate production by Gluconobacter oxydans or Pseudomonas sp. The productivity of the strain is also remarkably high at low pH, producing 146 g/l D-xylonate at pH 3.0. Comparison to production of D-xylonate from Saccharomyces cerevisiae strain VTT B-67002 expressing the gene from Caulobacter crescentus, overview
additional information
-
both D-xylono-gamma-lactone and D-xylonate are produced when the Caulobacter crescentus gene xylB encoding D-xylose dehydrogenase is expressed in Saccharomyces cerevisiae, with or without co-expressionof xylC (D-xylonolactonase). XylC facilitates rapid opening of the lactone and more D-xylonate is initially produced than in its absence. In vivo [1H]NMR analysis of cell extracts, culture media and intact cells is used for analysis. The lactone and linear forms of D-xylonic acid are produced, accumulated intracellularly, and partially exported within 1560 min after D-xylose provision. Co-expression of xylB and xylC leads to rapid loss of pHluorin fluorescence and loss of vitality during production of D-xylonate. Loss of vitality in the presence of D-xylose is correlated to the extracellular pH, but fluorescence is lost from xylB and xylC expressing cells regardless of the extracellular condition. Method optimization, overview; engineering of non-conventional yeast Pichia kudriavzevii strain VTT C-79090T to recombinantly express the D-xylose dehydrogenase coding gene from Caulobacter crescentus. With this single modification the recombinant strain VTT C-79090T produces up to 171 g/l of D-xylonate from 171 g/l D-xylose at a rate of 1.4 g/l/h and a high yield, which is comparable with D-xylonate production by Gluconobacter oxydans or Pseudomonas sp. The productivity of the strain is also remarkably high at low pH, producing 146 g/l D-xylonate at pH 3.0. Comparison to production of D-xylonate from Saccharomyces cerevisiae strain VTT B-67002 expressing the gene from Caulobacter crescentus, overview
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
65
half-life: 56.8 h, in the presence of 3 M NaCl, stability decreases significantly with lower salt concentrations
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
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CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
gene xylB, recombinnat expression in Pichia kudriavzevii strain VTT C-79090T
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gene xylB, recombinnat expression in Saccharomyces cerevisiae strain VVT B-67002
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
expression of gene xylB in Saccharomyces cerevisiae results in production of 17 g D-xylonate/l at 0.23g/l/h from 23 g D-xylose/l. D-Xylonate accumulates intracellularly to 70 mg/g, xylitol to 18 mg/g. Cells expressing D-xylonolactone lactonase xylC from Caulobacter crescentus with xylB initially produce more extracellular D-xylonate than cells lacking xylC at both pH5.5 and pH3, and sustain higher production at pH3. Cell vitality and viability decreases during D-xylonate production at pH 3.0
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yamanaka, K.; Gino, M.
Purification and properties of D-xylose dehydrogenase in bacteria
Hakko Kogaku Kaishi
57
322-331
1979
Pseudomonas sp.
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brenda
Yamanaka, K.; Gino, M.; Kaneda, R.
A specific NAD-D-xylose dehydrogenase from Arthrobacter sp.
Agric. Biol. Chem.
41
1493-1499
1977
Arthrobacter sp., Arthrobacter sp. 588
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brenda
Dahms, A.S.; Russo, J.
D-Xylose dehydrogenase
Methods Enzymol.
89
226-228
1982
Pseudomonas sp., Pseudomonas sp. MSU-1
brenda
Mostad, S.B.; Helming, H.L.; Groom, C.; Glasfeld, A.
The stereospecificity of hydrogen transfer to NAD(P)+ catalyzed by lactol dehydrogenases
Biochem. Biophys. Res. Commun.
233
681-686
1997
Sus scrofa
brenda
Bonete, M.J.; Pire, C.; Llorca, F.I.; Camacho, M.L.
Glucose dehydrogenase from the halophilic Archaeon Haloferax mediterranei: Enzyme purification, characterization and N-terminal sequence
FEBS Lett.
383
227-229
1996
Haloferax mediterranei (Q977U7), Haloferax mediterranei DSM 1411 (Q977U7)
brenda
Stephens, C.; Christen, B.; Fuchs, T.; Sundaram, V.; Watanabe, K.; Jenal, U.
Genetic analysis of a novel pathway for D-xylose metabolism in Caulobacter crescentus
J. Bacteriol.
189
2181-2185
2007
Caulobacter vibrioides, Caulobacter vibrioides NA1000
brenda
Toivari, M.; Nygard, Y.; Kumpula, E.P.; Vehkomaeki, M.L.; Bencina, M.; Valkonen, M.; Maaheimo, H.; Andberg, M.; Koivula, A.; Ruohonen, L.; Penttilae, M.; Wiebe, M.G.
Metabolic engineering of Saccharomyces cerevisiae for bioconversion of D-xylose to D-xylonate
Metab. Eng.
14
427-436
2012
Caulobacter vibrioides (B8H1Z0), Caulobacter vibrioides
brenda
Nygard, Y.; Maaheimo, H.; Mojzita, D.; Toivari, M.; Wiebe, M.; Resnekov, O.; Gustavo Pesce, C.; Ruohonen, L.; Penttilae, M.
Single cell and in vivo analyses elucidate the effect of xylC lactonase during production of D-xylonate in Saccharomyces cerevisiae
Metab. Eng.
25
238-247
2014
Caulobacter vibrioides (B8H1Z0), Caulobacter vibrioides NA1000 (B8H1Z0)
brenda
Toivari, M.; Vehkomaeki, M.; Nygard, Y.; Penttilae, M.; Ruohonen, L.; Wiebe, M.
Low pH D-xylonate production with Pichia kudriavzevii
Biores. Technol.
133
555-562
2013
Caulobacter vibrioides (B8H1Z0), Caulobacter vibrioides, Caulobacter vibrioides NA1000 (B8H1Z0)
brenda
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