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EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
ureidoglycolate dehydrogenase, (s)-ureidoglycolate dehydrogenase,
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(S)-ureidoglycolate dehydrogenase
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S-ureidoglycolate dehydrogenase
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(S)-ureidoglycolate + NAD(P)+ = oxalureate + NAD(P)H + H+
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(S)-ureidoglycolate:NAD(P)+ oxidoreductase
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(R,S)-ureidoglycolate + NAD+
oxalureate + NADH + H+
Arthrobacter allantoicus
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r
(S)-ureidoglycolate + NAD(P)+
oxalureate + NAD(P)H
Arthrobacter allantoicus
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strong substrate specificity, involved in the degradation of allantoin to glyoxylate in microbes and lower animals
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ir
(S)-ureidoglycolate + NAD+
oxalureate + NADH
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?
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(S)-ureidoglycolate + NAD(P)+
oxalureate + NAD(P)H
Arthrobacter allantoicus
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strong substrate specificity, involved in the degradation of allantoin to glyoxylate in microbes and lower animals
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ir
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NAD+
Arthrobacter allantoicus
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NADP+
Arthrobacter allantoicus
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glycolate
Arthrobacter allantoicus
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18% inhibition at 17 mM
glyoxylate
Arthrobacter allantoicus
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non-competitive inhibition, Ki: 83 mM
L-lactate
Arthrobacter allantoicus
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10% inhibition at 17 mM
Zn2+
Arthrobacter allantoicus
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40% inhibition at 0.5 mM
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9.1
(R,S)-ureidoglycolate
Arthrobacter allantoicus
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1.06 - 16.94
(S)-ureidoglycolate
0.1
NADP+
Arthrobacter allantoicus
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1.06
(S)-ureidoglycolate
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wild-type, pH 8.1, 30°C
5.27
(S)-ureidoglycolate
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mutant D141A, pH 8.1, 30°C
5.87
(S)-ureidoglycolate
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mutant D141N, pH 8.1, 30°C
10.75
(S)-ureidoglycolate
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mutant S43A, pH 8.1, 30°C
11.47
(S)-ureidoglycolate
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mutant R259A, pH 8.1, 30°C
12.38
(S)-ureidoglycolate
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mutant S140A, pH 8.1, 30°C
13.15
(S)-ureidoglycolate
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mutant M251A, pH 8.1, 30°C
14.13
(S)-ureidoglycolate
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mutant D141E, pH 8.1, 30°C
16.82
(S)-ureidoglycolate
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mutant Y52F, pH 8.1, 30°C
16.94
(S)-ureidoglycolate
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mutant H44A, pH 8.1, 30°C
0.37
NAD+
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mutant D141E, pH 8.1, 30°C
0.52
NAD+
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mutant D141A, pH 8.1, 30°C
0.56
NAD+
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wild-type, pH 8.1, 30°C
0.65
NAD+
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mutant H44A, pH 8.1, 30°C
0.81
NAD+
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mutant D141N, pH 8.1, 30°C
0.93
NAD+
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mutant M251A, pH 8.1, 30°C
1.26
NAD+
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mutant Y52F, pH 8.1, 30°C
1.39
NAD+
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mutant S140A, pH 8.1, 30°C
1.49
NAD+
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mutant R259A, pH 8.1, 30°C
2.16
NAD+
Arthrobacter allantoicus
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2.28
NAD+
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mutant S43A, pH 8.1, 30°C
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1.29 - 57
(S)-ureidoglycolate
1.29
(S)-ureidoglycolate
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mutant S140A, pH 8.1, 30°C
1.37
(S)-ureidoglycolate
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mutant S43A, pH 8.1, 30°C
1.62
(S)-ureidoglycolate
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mutant D141A, pH 8.1, 30°C
1.67
(S)-ureidoglycolate
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mutant D141E, pH 8.1, 30°C
1.76
(S)-ureidoglycolate
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mutant H44A, pH 8.1, 30°C
1.92
(S)-ureidoglycolate
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mutant Y52F, pH 8.1, 30°C
1.97
(S)-ureidoglycolate
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mutant D141N, pH 8.1, 30°C
11.98
(S)-ureidoglycolate
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mutant R259A, pH 8.1, 30°C
45.98
(S)-ureidoglycolate
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mutant M251A, pH 8.1, 30°C
57
(S)-ureidoglycolate
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wild-type, pH 8.1, 30°C
0.02
NAD+
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mutant S140A, pH 8.1, 30°C
0.87
NAD+
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mutant D141E, pH 8.1, 30°C
1.03
NAD+
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mutant H44A, pH 8.1, 30°C
1.06
NAD+
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mutant D141A, pH 8.1, 30°C
1.47
NAD+
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mutant D141N, pH 8.1, 30°C
1.9
NAD+
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mutant Y52F, pH 8.1, 30°C
2.51
NAD+
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mutant S43A, pH 8.1, 30°C
9.29
NAD+
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mutant R259A, pH 8.1, 30°C
30.65
NAD+
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mutant M251A, pH 8.1, 30°C
62
NAD+
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wild-type, pH 8.1, 30°C
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0.1 - 54
(S)-ureidoglycolate
0.1
(S)-ureidoglycolate
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mutant H44A, pH 8.1, 30°C
0.1
(S)-ureidoglycolate
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mutant S140A, pH 8.1, 30°C
0.11
(S)-ureidoglycolate
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mutant Y52F, pH 8.1, 30°C
0.12
(S)-ureidoglycolate
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mutant D141E, pH 8.1, 30°C
0.13
(S)-ureidoglycolate
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mutant S43A, pH 8.1, 30°C
0.31
(S)-ureidoglycolate
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mutant D141A, pH 8.1, 30°C
0.34
(S)-ureidoglycolate
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mutant D141N, pH 8.1, 30°C
1.04
(S)-ureidoglycolate
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mutant R259A, pH 8.1, 30°C
3.49
(S)-ureidoglycolate
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mutant M251A, pH 8.1, 30°C
54
(S)-ureidoglycolate
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wild-type, pH 8.1, 30°C
0.01
NAD+
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mutant S140A, pH 8.1, 30°C
1.1
NAD+
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mutant S43A, pH 8.1, 30°C
1.51
NAD+
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mutant Y52F, pH 8.1, 30°C
1.6
NAD+
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mutant H44A, pH 8.1, 30°C
1.81
NAD+
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mutant D141N, pH 8.1, 30°C
2.04
NAD+
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mutant D141A, pH 8.1, 30°C
2.35
NAD+
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mutant D141E, pH 8.1, 30°C
6.23
NAD+
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mutant R259A, pH 8.1, 30°C
32.9
NAD+
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mutant M251A, pH 8.1, 30°C
110
NAD+
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wild-type, pH 8.1, 30°C
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10.6
Arthrobacter allantoicus
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8 - 8.4
Arthrobacter allantoicus
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sensitive to ionic strength
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Arthrobacter allantoicus
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brenda
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brenda
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Arthrobacter allantoicus
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brenda
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crystal structure of AllD in its apo form is determined at 2.13 A resolution, as well as a binary complex at 1.64 A resolution with the NADH cofactor, and a ternary complex at 1.77 A resolution with NADH and glyoxylate, a product yielded from the spontaneous degradation of oxalurate
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D141A
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Km ((S)-ureidoglycolate) increased, kcat (S-ureidoglycolate) or (NAD+) decreased compared to wild-type, Km (NAD+) similar to wo wild-type
D141E
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Km ((S)-ureidoglycolate) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type, Km (NAD+) decreased compared to wild-type
D141N
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Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type
H116A
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mutant(S)-ureidoglycolate shows no activity
H44A
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Km ((S)-ureidoglycolate) increased, kcat (S-ureidoglycolate) or (NAD+) decreased compared to wild-type, Km (NAD+) equal to wild-type
M251A
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Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type
R259A
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Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type
R48A
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Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type
S140A
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Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type
S43A
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Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type
Y52F
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Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type
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6
Arthrobacter allantoicus
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decrease of activity below
286034
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30
Arthrobacter allantoicus
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unstable above
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incubation with 30% glycerol at 4°C for 3 h stabilizes activity
Arthrobacter allantoicus
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partial
Arthrobacter allantoicus
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expresed as a His-tagged fusion protein in Escherichia coli
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Van der Drift, C.; van Helvoort, P.E.M.; Vogels, G.D.
S-ureidoglycolate dehydrogenase: Purification and properties
Arch. Biochem. Biophys.
145
465-469
1971
Arthrobacter allantoicus
brenda
Rothe, M.; Alpert, C.; Loh, G.; Blaut, M.
Novel insights into E. colis hexuronate metabolism: KduI facilitates the conversion of galacturonate and glucuronate under osmotic stress conditions
PLoS ONE
8
e56906
2013
Escherichia coli
brenda
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1.1.1.154 ureidoglycolate dehydrogenase
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