Information on EC 1.1.1.150 - 21-hydroxysteroid dehydrogenase (NAD+)

for references in articles please use BRENDA:EC1.1.1.150
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

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EC NUMBER
COMMENTARY hide
1.1.1.150
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RECOMMENDED NAME
GeneOntology No.
21-hydroxysteroid dehydrogenase (NAD+)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
pregnan-21-ol + NAD+ = pregnan-21-al + NADH + H+
show the reaction diagram
Acts on a number of 21-hydroxycorticosteroids
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
-
-
redox reaction
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-
-
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reduction
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-
-
-
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SYSTEMATIC NAME
IUBMB Comments
21-hydroxysteroid:NAD+ 21-oxidoreductase
Acts on a number of 21-hydroxycorticosteroids.
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CAS REGISTRY NUMBER
COMMENTARY hide
37250-75-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
retinol dehydrogenase isoforms RDH12 and RDH13
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Manually annotated by BRENDA team
retinol dehydrogenase isoform RDH12
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Manually annotated by BRENDA team
Ovis aries aries
sheep
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-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione + NADH
11beta,17,21-trihydroxy-pregn-4,11-diene-3,20-dione + NAD+
show the reaction diagram
Ovis aries aries
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i.e. 21-dehydro-delta1-cortisol, only NAD dehydrogenase II
i.e. 21-hydroxy-delta1-cortisol
?
11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione + NADH
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NAD+
show the reaction diagram
11beta-hydroxy-pregn-4-ene-3,20,21-trione + NADH
11beta,21-dihydroxy-pregn-4-ene-3,20-dione + NAD+
show the reaction diagram
Ovis aries aries
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i.e. 21-dehydrocorticosterone, only NAD dehydrogenase II
i.e. 21-hydroxycorticosterone
?
17-hydroxy-pregn-4-ene-3,11,20,21-tetraone + NADH
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NAD+
show the reaction diagram
Ovis aries aries
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i.e. 21-dehydrocortisone
i.e. cortisone
?
17-hydroxy-pregn-4-ene-3,20,21-trione + NADH
17,21-dihydroxy-pregn-4-ene-3,20-dione + NAD+
show the reaction diagram
Ovis aries aries
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i.e. 21-dehydro-11-deoxycortisol, only NAD dehydrogenase II
i.e. 21-hydroxy-11-deoxycortisol
?
5beta-pregnane-3alpha,21-diol-20-one + NAD+
5beta-pregnane-3alpha-ol-20,21-dione + NADH + H+
show the reaction diagram
dihydrotestosterone + NADH
androstanediol + NAD+
show the reaction diagram
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substrate for retinol dehydrogenase isoform RDH12, no substrate for isoform RDH13
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-
?
pregn-4-ene-3,20,21-trione + NADH
21-hydroxy-pregn-4-ene-3,20-dione + NAD+
show the reaction diagram
Ovis aries aries
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i.e. 21-dehydro-11-deoxycorticosterone, only NAD dehydrogenase II
i.e. 21-hydroxy-11-deoxycorticosterone
?
additional information
?
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contrary to the human enzyme, dihydrotestosterone is not a substrate for mouse retinol dehydrogenase isoform RDH12
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
Ovis aries aries
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-
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
Ovis aries aries
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complete inhibition of NAD dehydrogenase II at 0.018 mM
Hg2+
Ovis aries aries
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40% inhibition of NAD dehydrogenase II at 0.004 mM
NAD+
Ovis aries aries
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NAD dehydrogenase II, competitive inhibition
NADP+
Ovis aries aries
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NAD dehydrogenase II, competitive inhibition
p-mercuribenzoate
Ovis aries aries
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79% inhibition of NAD dehydrogenase II at 0.014 mM
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.172
11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione
Ovis aries aries
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NAD dehydrogenase II
0.182
11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione
Ovis aries aries
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0.105
11beta-hydroxy-pregn-4-ene-3,20,21-trione
Ovis aries aries
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NAD dehydrogenase II
0.221
17-hydroxy-pregn-4-ene-3,11,20,21-tetraone
Ovis aries aries
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0.111
17-hydroxy-pregn-4-ene-3,20,21-trione
Ovis aries aries
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NAD dehydrogenase II
0.01 - 0.048
NADH
0.174
pregn-4-ene-3,20,21-trione
Ovis aries aries
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NAD dehydrogenase II
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2
Ovis aries aries
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NAD dehydrogenase II
6.9
Ovis aries aries
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NAD dehydrogenase I
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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isoform RDH13
Manually annotated by BRENDA team
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56
Ovis aries aries
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30% loss of activity in 5 min, NAD dehydrogenase I more heat resistant than NAD dehydrogenase II
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity, gel filtration, starch block electrophoresis
Ovis aries aries
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in HeLa cell and 293T cell
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LINKS TO OTHER DATABASES (specific for EC-Number 1.1.1.150)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)