Information on EC 1.1.1.130 - 3-dehydro-L-gulonate 2-dehydrogenase

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The expected taxonomic range for this enzyme is: Klebsiella aerogenes

EC NUMBER
COMMENTARY hide
1.1.1.130
-
RECOMMENDED NAME
GeneOntology No.
3-dehydro-L-gulonate 2-dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-dehydro-L-gulonate + NAD(P)+ = (4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate + NAD(P)H + H+
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
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redox reaction
-
-
-
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reduction
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-ascorbate degradation II (bacterial, aerobic)
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ascorbate metabolism
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Pentose and glucuronate interconversions
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Ascorbate and aldarate metabolism
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SYSTEMATIC NAME
IUBMB Comments
3-dehydro-L-gulonate:NAD(P)+ 2-oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
37250-61-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate + NAD(P)H
3-dehydro-L-gulonate + NAD(P)+
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate + NAD(P)H
3-dehydro-L-gulonate + NAD(P)+
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
twice effective compared to NADPH
NADPH
-
NADH 2fold more effective
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
p-chloromercuribenzoate
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32% inhibition at 1 mM, not reversal by GSH
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.16
(4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate
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-
0.016
NADH
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-
0.022
NADPH
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2 - 8.4
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50% of maximal activity at pH 5.2 and pH 8.4
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
PDB
SCOP
CATH
UNIPROT
ORGANISM
Escherichia coli (strain K12);
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, 0.03 M Tris-HCl, pH 7.7, 0.62 mg protein/ml, 85% retaining activity in 4 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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