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EC Tree
The expected taxonomic range for this enzyme is: Archaea, Eukaryota
Synonyms
L-xylose dehydrogenase, NADPH-preferring xylose reductase, NADPH-xylose reductase, xylose reductase,
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L-xylose dehydrogenase
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NADPH-preferring xylose reductase
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NADPH-xylose reductase
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L-xylose + NADP+ = L-xylono-1,4-lactone + NADPH + H+
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L-xylose:NADP+ 1-oxidoreductase
Also oxidizes D-arabinose and D-lyxose.
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D-arabinose + NADP+
D-arabinono-1,4-lactone + NADPH
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D-lyxose + NADP+
D-lyxono-1,4-lactone + NADPH
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L-xylose + NADP+
L-xylono-1,4-lactone + NADPH
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L-xylose + NADP+
L-xylono-1,4-lactone + NADPH + H+
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D-xylose + NADP+
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88% of the activity with D-glucose and NADP+. The enzyme shows also activity with D-xylose and NAD+ as cofactor, D-glucose (NADP+ or NAD+ as cofactor), D-fucose (cofactor NADP+) and D-galactose (cofactor NADP+)
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D-xylose + NADP+
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88% of the activity with D-glucose and NADP+. The enzyme shows also activity with D-xylose and NAD+ as cofactor, D-glucose (NADP+ or NAD+ as cofactor), D-fucose (cofactor NADP+) and D-galactose (cofactor NADP+)
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L-xylose + NADP+
L-xylono-1,4-lactone + NADPH
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L-xylose + NADP+
L-xylono-1,4-lactone + NADPH + H+
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NADPH
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preferred cofactor
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SwissProt
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SwissProt
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A0A1B4XTS0_9ASCO
362
0
38984
TrEMBL
other Location (Reliability: 1 )
GLCDH_HALMT
Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4)
357
0
39242
Swiss-Prot
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53000
2 * 53000, SDS-PAGE
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dimer
2 * 53000, SDS-PAGE
dimer
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2 * 53000, SDS-PAGE
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K270D/N272P/S271G/R276F
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mutant shows a decreased NADPH and NADH xylose reductase activities, respectively, compared to wild-type
K270G
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mutant shows a decreased NADPH and NADH xylose reductase activities, respectively, compared to wild-type
K270M
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mutant shows a decreased NADPH and NADH xylose reductase activities, respectively, compared to wild-type
K270R
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mutant shows a 3.4fold and 2.4fold higher NADPH and NADH preferred xylose reductase activities, respectively, compared with wild-type. Mutant exhibits an improved xylose consumption rate, balanced redox system and increased ethanol yield and production rate
K270R/N272D
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mutant shows a decreased NADPH and NADH xylose reductase activities, respectively, compared to wild-type
K270R/R276H
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mutant shows a decreased NADPH and NADH xylose reductase activities, respectively, compared to wild-type
K274R
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mutant shows a decreased NADPH and NADH xylose reductase activities, respectively, compared to wild-type
K274R/N276D
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mutant shows a decreased NADPH and NADH xylose reductase activities, respectively, compared to wild-type
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37 - 45
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after 10 min at 37°C, 42°C, and 45°C, the retained activities of the enzyme are 97.17%, 101.10% and 87.35%, respectively
65
half-life: 56.8 h, in the presence of 3 M NaCl, stability decreases significantly with lower salt concentrations
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4°C, stable for several months
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ammonium sulfate, acetone, DEAE-cellulose
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expressed in Kluyveromyces marxianus strain YZJ088
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Uehara, K.; Takeda, M.
L-Xylose dehydrogenase in baker's yeast
J. Biochem.
52
461-463
1962
Saccharomyces cerevisiae
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Bonete, M.J.; Pire, C.; Llorca, F.I.; Camacho, M.L.
Glucose dehydrogenase from the halophilic Archaeon Haloferax mediterranei: Enzyme purification, characterization and N-terminal sequence
FEBS Lett.
383
227-229
1996
Haloferax mediterranei (Q977U7), Haloferax mediterranei DSM 1411 (Q977U7)
brenda
Xiong, M.; Woodruff, A.; Tang, X.; Tian, X.; Zhang, J.; Cao, L.
Comparative study on the mutated xylose reductase to increase ethanol production in xylose-utilizing Saccharomyces cerevisiae strains
J. Taiwan Inst. Chem. Eng.
44
605-610
2013
Saccharomyces cerevisiae
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brenda
Zhang, J.; Zhang, B.; Wang, D.; Gao, X.; Sun, L.; Hong, J.
Rapid ethanol production at elevated temperatures by engineered thermotolerant Kluyveromyces marxianus via the NADP(H)-preferring xylose reductase-xylitol dehydrogenase pathway
Metab. Eng.
31
140-152
2015
Neurospora crassa
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