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EC 2.7.9.6 Details

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EC number

2.7.9.6

Accepted name

rifampicin phosphotransferase

Reaction

ATP + rifampicin + H₂O = AMP + 21-phosphorifampicin + phosphate

Other name(s)

rifampin phosphotransferase, RPH

Systematic name

ATP:rifampicin, water 21-O-phosphotransferase

Comment

The enzyme, characterized from a diverse collection of Gram-positive bacteria, inactivates the antibiotic rifampicin by phosphorylating it at position 21. The enzyme comprises three domains: two substrate-binding domains (ATP-grasp and rifampicin-binding domains) and a smaller phosphate-carrying L-histidine swivel domain that transits between the spatially distinct substrate-binding sites during catalysis.

History

created 2018

EC Tree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.2 Acting on the aldehyde or oxo group of donors

1.3 Acting on the CH-CH group of donors

1.4 Acting on the CH-NH₂ group of donors

1.5 Acting on the CH-NH group of donors

1.6 Acting on NADH or NADPH

1.7 Acting on other nitrogenous compounds as donors

1.8 Acting on a sulfur group of donors

1.9 Acting on a heme group of donors

1.10 Acting on diphenols and related substances as donors

1.11 Acting on a peroxide as acceptor

1.12 Acting on hydrogen as donor

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.1 With NADH or NADPH as one donor (deleted sub-subclass)

1.14.2 With ascorbate as one donor (deleted sub-subclass)

1.14.3 With reduced pteridine as one donor (deleted sub-subclass)

1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor

1.14.16 With reduced pteridine as one donor, and incorporation of one atom of oxygen into the other donor

1.14.17 With reduced ascorbate as one donor, and incorporation of one atom of oxygen into the other donor

1.14.18 With another compound as one donor, and incorporation of one atom of oxygen into the other donor

1.14.19 With oxidation of a pair of donors resulting in the reduction of O₂ to two molecules of water

1.14.20 With 2-oxoglutarate as one donor, and the other dehydrogenated

1.14.20.1 deacetoxycephalosporin-C synthase

1.14.20.2 2,4-dihydroxy-1,4-benzoxazin-3-one-glucoside dioxygenase

1.14.20.3 (5R)-carbapenem-3-carboxylate synthase

1.14.20.4 anthocyanidin synthase

1.14.20.5 flavone synthase I

1.14.20.6 flavonol synthase

1.14.20.7 2-oxoglutarate/L-arginine monooxygenase/decarboxylase (succinate-forming)

1.14.20.8 (–)-deoxypodophyllotoxin synthase

1.14.20.9 L-tyrosine isonitrile desaturase

1.14.20.10 L-tyrosine isonitrile desaturase/decarboxylase

1.14.20.11 3-[(Z)-2-isocyanoethenyl]-1H-indole synthase

1.14.20.12 3-[(E)-2-isocyanoethenyl]-1H-indole synthase

1.14.20.13 6β-hydroxyhyoscyamine epoxidase

1.14.20.14 hapalindole-type alkaloid chlorinase

1.14.20.15 L-threonyl-[L-threonyl-carrier protein] 4-chlorinase

1.14.21 With NADH or NADPH as one donor, and the other dehydrogenated

1.14.99 Miscellaneous

1.15 Acting on superoxide as acceptor

1.16 Oxidizing metal ions

1.17 Acting on CH or CH₂ groups

1.18 Acting on iron-sulfur proteins as donors

1.19 Acting on reduced flavodoxin as donor

1.20 Acting on phosphorus or arsenic in donors

1.21 Catalysing the reaction X-H + Y-H = X-Y

1.22 Acting on halogen in donors

1.23 Reducing C-O-C group as acceptor

1.23.1 With NADH or NADPH as donor

1.23.5 With a quinone or similar compound as acceptor

1.97 Other oxidoreductases

1.98 Enzymes using H₂ as reductant (deleted subclass)

1.99 Other enzymes using O₂ as oxidant (deleted subclass)