EC Explorer

[browse EC tree] - [search]

EC 1.14.13.100 Details

show Brenda entry

EC number

1.14.13.100

Accepted name

25/26-hydroxycholesterol 7α-hydroxylase

Reaction

(1) cholest-5-ene-3β,25-diol + NADPH + H⁺ + O₂ = cholest-5-ene-3β,7α,25-triol + NADP⁺ + H₂O;;(2) (25R)-cholest-5-ene-3β,26-diol + NADPH + H⁺ + O₂ = (25R)-cholest-5-ene-3β,7α,26-triol + NADP⁺ + H₂O

Other name(s)

25-hydroxycholesterol 7α-monooxygenase, CYP7B1, CYP7B1 oxysterol 7α-hydroxylase, 27-hydroxycholesterol 7-monooxygenase, 27-hydroxycholesterol 7α-hydroxylase, cholest-5-ene-3β,25-diol,NADPH:oxygen oxidoreductase (7α-hydroxylating), 25-hydroxycholesterol 7α-hydroxylase

Systematic name

cholest-5-ene-3β,25/26-diol,NADPH:oxygen oxidoreductase (7α-hydroxylating)

CAS registry number

149316-80-3

Comment

A heme-thiolate protein (P-450). Unlike EC 1.14.13.99, 24-hydroxycholesterol 7α-monooxygenase, which is specific for its oxysterol substrate, this enzyme can also metabolize the oxysterols 24,25-epoxycholesterol, 22-hydroxycholesterol and 24-hydroxycholesterol, but to a lesser extent [2].

History

created 2005, modified 2013 (EC 1.14.13.60 created 1999, incorporated 2013), deleted 2016

EC Tree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.2 Acting on the aldehyde or oxo group of donors

1.3 Acting on the CH-CH group of donors

1.4 Acting on the CH-NH₂ group of donors

1.5 Acting on the CH-NH group of donors

1.6 Acting on NADH or NADPH

1.7 Acting on other nitrogenous compounds as donors

1.8 Acting on a sulfur group of donors

1.9 Acting on a heme group of donors

1.10 Acting on diphenols and related substances as donors

1.11 Acting on a peroxide as acceptor

1.12 Acting on hydrogen as donor

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.1 With NADH or NADPH as one donor (deleted sub-subclass)

1.14.2 With ascorbate as one donor (deleted sub-subclass)

1.14.3 With reduced pteridine as one donor (deleted sub-subclass)

1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor

1.14.16 With reduced pteridine as one donor, and incorporation of one atom of oxygen into the other donor

1.14.16.1 phenylalanine 4-monooxygenase

1.14.16.2 tyrosine 3-monooxygenase

1.14.16.3 anthranilate 3-monooxygenase

1.14.16.4 tryptophan 5-monooxygenase

1.14.16.5 alkylglycerol monooxygenase

1.14.16.6 mandelate 4-monooxygenase

1.14.16.7 phenylalanine 3-monooxygenase

1.14.17 With reduced ascorbate as one donor, and incorporation of one atom of oxygen into the other donor

1.14.18 With another compound as one donor, and incorporation of one atom of oxygen into the other donor

1.14.19 With oxidation of a pair of donors resulting in the reduction of O₂ to two molecules of water

1.14.20 With 2-oxoglutarate as one donor, and the other dehydrogenated

1.14.21 With NADH or NADPH as one donor, and the other dehydrogenated

1.14.99 Miscellaneous

1.15 Acting on superoxide as acceptor

1.16 Oxidizing metal ions

1.17 Acting on CH or CH₂ groups

1.18 Acting on iron-sulfur proteins as donors

1.19 Acting on reduced flavodoxin as donor

1.20 Acting on phosphorus or arsenic in donors

1.21 Catalysing the reaction X-H + Y-H = X-Y

1.22 Acting on halogen in donors

1.23 Reducing C-O-C group as acceptor

1.23.1 With NADH or NADPH as donor

1.23.5 With a quinone or similar compound as acceptor

1.97 Other oxidoreductases

1.98 Enzymes using H₂ as reductant (deleted subclass)

1.99 Other enzymes using O₂ as oxidant (deleted subclass)