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EC 1.14.13.88 Details
EC number
1.14.13.88
Accepted name
flavanoid 3′,5′-hydroxylase
Reaction
a flavanone + 2 NADPH + 2 H+ + 2 O2 = a 3′,5′-dihydroxyflavanone + 2 NADP+ + 2 H2O (overall reaction);;(1a) a flavanone + NADPH + H+ + O2 = a 3′-hydroxyflavanone + NADP+ + H2O;;(1b) a 3′-hydroxyflavanone + NADPH + H+ + O2 = a 3′,5′-dihydroxyflavanone + NADP+ + H2O
Other name(s)
flavonoid 3′,5′-hydroxylase
Systematic name
flavanone,NADPH:oxygen oxidoreductase
CAS registry number
94047-23-1
Comment
A heme-thiolate protein (P-450). The 3′,5′-dihydroxyflavanone is formed via the 3′-hydroxyflavanone. In Petunia hybrida the enzyme acts on naringenin, eriodictyol, dihydroquercetin (taxifolin) and dihydrokaempferol (aromadendrin). The enzyme catalyses the hydroxylation of 5,7,4′-trihydroxyflavanone (naringenin) at either the 3′ position to form eriodictyol or at both the 3′ and 5′ positions to form 5,7,3′,4′,5′-pentahydroxyflavanone (dihydrotricetin). The enzyme also catalyses the hydroxylation of 3,5,7,3′,4′-pentahydroxyflavanone (taxifolin) at the 5′ position, forming ampelopsin. NADH is not a good substitute for NADPH.
History
created 2004, deleted 2018
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