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EC 3.5.1.119 Details
EC number
3.5.1.119
Accepted name
Pup amidohydrolase
Reaction
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O = [prokaryotic ubiquitin-like protein]-L-glutamate + NH3
Other name(s)
dop (gene name), Pup deamidase, depupylase/deamidase, DPUP, depupylase
Systematic name
[prokaryotic ubiquitin-like protein]-L-glutamine amidohydrolase
Comment
The enzyme has been characterized from the bacterium Mycobacterium tuberculosis. It catalyses the hydrolysis of the amido group of the C-terminal glutamine of prokaryotic ubiquitin-like protein (Pup), thus activating it for ligation to target proteins, a process catalysed by EC 6.3.1.19, prokaryotic ubiquitin-like protein ligase. The reaction requires ATP as cofactor but not its hydrolysis. The enzyme also catalyses the hydrolytic cleavage of the bond formed by the ligase, between an ε-amino group of a lysine residue of the target protein and the γ-carboxylate of the C-terminal glutamate of the prokaryotic ubiquitin-like protein.
History
created 2015
EC Tree
1.14.13.45 created 1992, deleted 2003