EC Explorer

3.5.1.114

N-acyl-aromatic-L-amino acid amidohydrolase

(1) an N-acyl-aromatic-L-amino acid + H₂O = an aromatic-L-amino acid + a carboxylate;;(2) an N-acetyl-L-cysteine-S-conjugate + H₂O = an L-cysteine-S-conjugate + acetate

aminoacylase 3, aminoacylase III, ACY3 (gene name)

N-acyl-aromatic-L-amino acid amidohydrolase (carboxylate-forming)

This enzyme is found in animals and is involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). It preferentially deacetylates N^α-acetylated aromatic amino acids and mercapturic acids (S-conjugates of N-acetyl-L-cysteine) that are usually not deacetylated by EC 3.5.1.14, N-acyl-aliphatic-L-amino acid amidohydrolase. The enzyme is significantly activated by Co²⁺ and Ni²⁺ [3]. Some bacterial aminoacylases demonstrate substrate specificity for both EC 3.5.1.14 and EC 3.5.1.114. cf. EC 3.5.1.14, N-acyl-aliphatic-L-amino acid amidohydrolase and EC 3.5.1.15, aspartoacylase.

created 2013