Any feedback?
Please rate this page
(ecexplorer.php)
(0/150)

BRENDA support

EC Explorer

EC 1.17.99.3 Details
EC number
1.17.99.3
Accepted name
3α,7α,12α-trihydroxy-5β-cholestanoyl-CoA 24-hydroxylase
Reaction
(25R)-3α,7α,12α-trihydroxy-5β-cholestan-26-oyl-CoA + H2O + acceptor = (24R,25R)-3α,7α,12α,24-tetrahydroxy-5β-cholestan-26-oyl-CoA + reduced acceptor
Other name(s)
trihydroxycoprostanoyl-CoA oxidase, THC-CoA oxidase, THCA-CoA oxidase, 3α,7α,12α-trihydroxy-5β-cholestanoyl-CoA oxidase, 3α,7α,12α-trihydroxy-5β-cholestan-26-oate 24-hydroxylase
Systematic name
(25R)-3α,7α,12α-trihydroxy-5β-cholestan-26-oyl-CoA:acceptor 24-oxidoreductase (24R-hydroxylating)
CAS registry number
119799-47-2
Comment
Requires ATP. The reaction in mammals possibly involves dehydrogenation to give a 24(25)-double bond followed by hydration [1]. However, in amphibians such as the Oriental fire-bellied toad (Bombina orientalis), it is probable that the product is formed via direct hydroxylation of the saturated side chain of (25R)-3α,7α,12α-trihydroxy-5β-cholestan-26-oate and not via hydration of a 24(25) double bond [5]. In microsomes, the free acid is preferred to the coenzyme A ester, whereas in mitochondria, the coenzyme A ester is preferred to the free-acid form of the substrate [1].
History
created 2005
EC Tree