EC Explorer
[browse EC tree] - [search]
EC 6.1.2.2 Details
show Brenda entryEC number
6.1.2.2
Accepted name
nebramycin 5′ synthase
Reaction
(1) tobramycin + carbamoyl phosphate + ATP + H2O = nebramycin 5′ + AMP + diphosphate + phosphate (overall reaction);;(1a) carbamoyl phosphate + ATP + H2O = diphosphate + O-carbamoyladenylate + phosphate;;(1b) O-carbamoyladenylate + tobramycin = AMP + nebramycin 5′ ;;(2) kanamycin A + carbamoyl phosphate + ATP + H2O = 6′′-O-carbamoylkanamycin A + AMP + diphosphate + phosphate (overall reaction);;(2a) carbamoyl phosphate + ATP + H2O = diphosphate + O-carbamoyladenylate + phosphate;;(2b) O-carbamoyladenylate + kanamycin A = AMP + 6′′-O-carbamoylkanamycin A
Other name(s)
tobramycin carbamoyltransferase,
TobZ
Systematic name
tobramycin:carbamoyl phosphate ligase (AMP,phosphate-forming)
Comment
Requires Fe(III). The enzyme from the bacterium Streptoalloteichus tenebrarius catalyses the activation of carbamoyl phosphate to O-carbamoyladenylate and the subsequent carbamoylation of kanamycin and tobramycin.
History
created 2014
back to topEC Tree
1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.14.1.2 created 1965, deleted 1972 1.14.1.4 created 1965, deleted 1972 1.14.1.5 created 1965, deleted 1972 1.14.1.7 created 1965, deleted 1972 1.14.1.8 created 1965, deleted 1972 1.14.1.9 created 1965, deleted 1972 1.14.1.10 created 1965, deleted 1972 1.14.1.11 created 1965, deleted 1972
